Characterization and Heterologous Expression of the Oxalyl Coenzyme A Decarboxylase Gene from
            <i>Bifidobacterium lactis</i>

Federici, Federica; Vitali, Beatrice; Gotti, Roberto; Pasca, Maria Rosalia; Gobbi, Silvia; Peck, Ammon B.; Brigidi, Patrizia

doi:10.1128/aem.70.9.5066-5073.2004

Cited by 68 publications

(66 citation statements)

References 46 publications

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“…We do not know that the strain of B. infantis present in Oxadrop is similar in its oxalate-degrading properties to a strain of the same species that degraded 26.7% of a given amount of oxalate, compared with 60.6% for a B. lactis strain and 100% for O. formigenes (10). In that same study, another strain of B. infantis degraded only 4% of the oxalate in the culture medium, not much more than the negative control, E. coli.…”

Section: Discussionmentioning

confidence: 99%

“…Some lactic acid bacterium species do possess enzymes that are at least partially homologous to those demonstrated in O. formigenes. For instance, a strain of Bifidobacterium lactis isolated from yogurt showed oxalate-degrading activity in vitro and then was shown to have a gene 47% homologous with oxc from O. formigenes, which produced an active enzyme (10). Because lactic acid bacteria use carbohydrate as their main substrate, whereas O. formigenes metabolizes only oxalate, the relative contribution of any oxalate utilization by the former may be substantially less.…”

Section: Discussionmentioning

confidence: 99%

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A Randomized, Controlled Trial of Lactic Acid Bacteria for Idiopathic Hyperoxaluria

Goldfarb

Modersitzki²,

Asplin³

2007

Clinical Journal of the American Society of Nephrology

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Background: Urinary oxalate excretion is an important contributor to calcium oxalate stone formation. Methods of reducing oxalate excretion are not wholly satisfactory, and no controlled trials using them have been performed to prevent stone recurrence. Some lactic acid bacteria can degrade oxalate in vitro. This study sought to reduce urinary oxalate excretion in calcium stone formers with idiopathic hyperoxaluria.Design, setting, participants, and measurements: A randomized, double-blind, placebo-controlled trial was performed of Oxadrop, a mix of four lactic acid bacterium species. This preparation previously reduced oxalate excretion in stone formers with idiopathic and enteric hyperoxaluria. Patients were selected from two stone prevention clinics. Twenty people with calcium stones and idiopathic hyperoxaluria (>40 mg/d) were enrolled and randomly assigned 1:1 in placebo and active preparation arms. Both groups took 3.6 g of granulate each day: Either placebo or the experimental preparation. Participants performed two consecutive 24-h urine collections at baseline, at 28 d of therapy, and at 56 d, after being off the preparation for 4 wk. Diet was replicated at each point.Results: There was no effect of the study preparation: Mean 24-h urinary oxalate excretion in placebo-treated patients was 73.9 mg at baseline and 72.7 mg after treatment, whereas the Oxadrop-treated patients had 59.1 mg at baseline and 55.4 mg after treatment. The preparation was well tolerated; three participants on active treatment experienced mild constipation.Conclusions: In this randomized, placebo-controlled trial, Oxadrop did not reduce urinary oxalate excretion in participants with idiopathic hyperoxaluria.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Randomized, Controlled Trial of Lactic Acid Bacteria for Idiopathic Hyperoxaluria

Goldfarb

Modersitzki²,

Asplin³

2007

Clinical Journal of the American Society of Nephrology

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“…LBA0041 is predicted to encode a putative adenosylcobalamin-dependent ribonucleoside triphosphate reductase. ORFs LBA0038, LBA0039, and LBA0040 are poorly characterized; however, the LBA0040 product is similar to a putative ATP:cob(I)alamin adenosyltransferase (23), the enzyme responsible for the last step in the activation of vitamin B 12 (cyanocobalamin) to coenzyme B 12 (adenosylcobalamin). A relationship between altered oxalate metabolism and B vitamin deficiency has been documented (3,18), which resulted in some interest in why these genes are upregulated in the presence of ammonium oxalate.…”

Section: Analysis Of the Chromosomal Region Containing Frc And Oxcmentioning

confidence: 99%

“…A conserved domain (pfam02515) belonging to a new family of CoA (27) and B. lactis (46% identity and 63% similarity) (12). The protein encoded by oxc has a conserved domain that is present in thiamine pyrophosphate (TPP)-requiring enzymes (COG0028).…”

Section: Analysis Of the Chromosomal Region Containing Frc And Oxcmentioning

confidence: 99%

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Transcriptional and Functional Analysis of Oxalyl-Coenzyme A (CoA) Decarboxylase and Formyl-CoA Transferase Genes from Lactobacillus acidophilus

Azcarate‐Peril

Bruno-Bárcena²,

Hassan³

et al. 2006

Appl Environ Microbiol

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Oxalic acid is found in dietary sources (such as coffee, tea, and chocolate) or is produced by the intestinal microflora from metabolic precursors, like ascorbic acid. In the human intestine, oxalate may combine with calcium, sodium, magnesium, or potassium to form less soluble salts, which can cause pathological disorders such as hyperoxaluria, urolithiasis, and renal failure in humans. In this study, an operon containing genes homologous to a formyl coenzyme A transferase gene (frc) and an oxalyl coenzyme A decarboxylase gene (oxc) was identified in the genome of the probiotic bacterium Lactobacillus acidophilus. Physiological analysis of a mutant harboring a deleted version of the frc gene confirmed that frc expression specifically improves survival in the presence of oxalic acid at pH 3.5 compared with the survival of the wild-type strain. Moreover, the frc mutant was unable to degrade oxalate. These genes, which have not previously been described in lactobacilli, appear to be responsible for oxalate degradation in this organism. Transcriptional analysis using cDNA microarrays and reverse transcription-quantitative PCR revealed that mildly acidic conditions were a prerequisite for frc and oxc transcription. As a consequence, oxalate-dependent induction of these genes occurred only in cells first adapted to subinhibitory concentrations of oxalate and then exposed to pH 5.5. Where genome information was available, other lactic acid bacteria were screened for frc and oxc genes. With the exception of Lactobacillus gasseri and Bifidobacterium lactis, none of the other strains harbored genes for oxalate utilization.

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Plant Polyphenols Stimulate Adhesion to Intestinal Mucosa and Induce Proteome Changes in the Probiotic Lactobacillus acidophilus NCFM

Çelebioğlu

Delsoglio

Brix

et al. 2018

Molecular Nutrition Food Res

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Characterization and Heterologous Expression of the Oxalyl Coenzyme A Decarboxylase Gene from Bifidobacterium lactis

Cited by 68 publications

References 46 publications

A Randomized, Controlled Trial of Lactic Acid Bacteria for Idiopathic Hyperoxaluria

A Randomized, Controlled Trial of Lactic Acid Bacteria for Idiopathic Hyperoxaluria

Transcriptional and Functional Analysis of Oxalyl-Coenzyme A (CoA) Decarboxylase and Formyl-CoA Transferase Genes from Lactobacillus acidophilus

Plant Polyphenols Stimulate Adhesion to Intestinal Mucosa and Induce Proteome Changes in the Probiotic Lactobacillus acidophilus NCFM

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