Characterization and husbandry of wild broodstock of the blackfin icefish Chaenocephalus aceratus (Lönnberg 1906) from the Palmer Archipelago (Southern Ocean) for breeding purposes

François, Nathalie; Sheehan, Eileen; Desvignes, Thomas; Belzile, Claude; Postlethwait, John H.; Detrich, H. William

doi:10.1007/s00300-017-2161-9

Cited by 8 publications

(4 citation statements)

References 56 publications

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“…Additional C. aceratus were collected for construction of a genetic linkage map, and for miRNA sequencing in May of 2012-2015 by bottom trawling from the Antarctic Research and Supply Vessel Laurence M. Gould at depths of 160-200 m southwest of Low Island (Antarctic Specially Protected Area 152, Western Bransfield Strait; latitudes 63° 15′ S-63° 30′ S; longitudes 62° 00′ W-62° 45′ W, bounded on the northeast by Low Island)). These fish were transported alive to Palmer Station, Antarctica, where they were maintained in seawater aquaria at −1-0 °C following best practices for maintaining icefishes in captivity 56 .…”

Section: Methodsmentioning

confidence: 99%

Antarctic blackfin icefish genome reveals adaptations to extreme environments

et al. 2019

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Icefishes (suborder Notothenioidei; family Channichthyidae) are the only vertebrates that lack functional haemoglobin genes and red blood cells. Here, we report a high-quality genome assembly and linkage map for the Antarctic blackfin icefish Chaenocephalus aceratus, highlighting evolved genomic features for its unique physiology. Phylogenomic analysis revealed that Antarctic fish of the teleost suborder Notothenioidei, including icefishes, diverged from the stickleback lineage about 77 million years ago and subsequently evolved cold-adapted phenotypes as the Southern Ocean cooled to sub-zero temperatures. Our results show that genes involved in protection from ice damage, including genes encoding antifreeze glycoprotein and zona pellucida proteins, are highly expanded in the icefish genome. Furthermore, genes that encode enzymes that help to control cellular redox state, including members of the sod3 and nqo1 gene families, are expanded, probably as evolutionary adaptations to the relatively high concentration of oxygen dissolved in cold Antarctic waters. In contrast, some crucial regulators of circadian homeostasis (cry and per genes) are absent from the icefish genome, suggesting compromised control of biological rhythms in the polar light environment. The availability of the icefish genome sequence will accelerate our understanding of adaptation to extreme Antarctic environments.

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Section: Methodsmentioning

confidence: 99%

Antarctic blackfin icefish genome reveals adaptations to extreme environments

et al. 2019

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“…Blackfin icefish species have thin, highly vascularized, scaleless skin; elongated bodies; and a weaker skeleton in comparison with most red-blooded notothenioid species. Their body structure makes them extremely vulnerable to injury 4 . Icefish, also known as white-blooded fish, belong to a unique family in that they are the only known vertebrates to lack hemoglobin.…”

Section: Background and Summarymentioning

confidence: 99%

A chromosome-level reference genome of the Antarctic blackfin icefish Chaenocephalus aceratus

Lee,

Kim,

Choi

et al. 2023

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The blackfin Icefish (Chaenocephalus aceratus) belongs to the family Channichthyidae and the suborder Notothenioidei which lives in the Antarctic. We corrected the mis-scaffolds in the previous linkage map results by Hi-C analysis to obtain improved results for chromosome-level genome assembly. The final assembly analysis resulted in a total of 3,135 scaffolds, a genome size of 1,065.72 Mb, and an N50 of 33.46 Mb. 820.24 Mb, representing 88.88% of the total genome, is anchored to 24 chromosomes. The final gene set of 38,024 genes, including AFGPs, was annotated using RNA evidence, proteins, and ab-initio predictions. The complete percentage of BUSCO analysis is 92.7%. In this study, we aim to contribute to the study of polar fishes by improving the genome sequences of the blackfin icefish with the AFGP genes belonging to the Notothenoidei.

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“…Fish were transferred immediately from the trawl net to the ship aquaria (1 m 3 Xactics TM , Cornwall, Ontario, Canada) supplied with flow-through seawater with enhanced aeration as previously described 37 . After about a day or two at sea, live fish were transported to the aquatic facilities at Palmer Station, Antarctica, where they were maintained in 2.5 m 3 flow-through seawater tanks at ambient temperatures of −1 to +1 °C following husbandry guidelines previously described 38 . Upon arrival on station, potentially sexually mature C .…”

Section: Methodsmentioning

confidence: 99%

Intergeneric hybrids inform reproductive isolating barriers in the Antarctic icefish radiation

Desvignes

François

Goetz

et al. 2019

Sci Rep

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Interspecific hybridization or barriers to hybridization may have contributed to the diversification of Antarctic icefishes (Channichthyidae), but data supporting these hypotheses is scarce. To understand the potential for hybridization and to investigate reproductive isolating mechanisms among icefish species, we performed in vitro fertilization experiments using eggs from a female blackfin icefish Chaenocephalus aceratus and sperm from a male of another genera, the ocellated icefish Chionodraco rastrospinosus . Sequencing of genomic and mitochondrial DNA confirmed the intergeneric hybrid nature of resulting embryos which successfully developed and hatched as active larvae at about four and a half months during the Antarctic winter. This result demonstrates the compatibility of gametes of these two species and the viability of resulting zygotes and larvae. Due to logistic constraints and the slow developmental rate of icefishes, we could not test for long-term hybrid viability, fertility, fitness, or hybrid breakdown. Analysis of our fishing records and available literature, however, suggests that the strongest barriers to hybridization among parapatric icefish species are likely to be behavioral and characterized by assortative mating and species-specific courtship and nesting behaviors. This conclusion suggests that, in long-lived fish species with late sexual maturity and high energetic investment in reproduction like icefishes, pre-mating barriers are energetically more efficient than post-mating barriers to prevent hybridization.

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Characterization and husbandry of wild broodstock of the blackfin icefish Chaenocephalus aceratus (Lönnberg 1906) from the Palmer Archipelago (Southern Ocean) for breeding purposes

Cited by 8 publications

References 56 publications

Antarctic blackfin icefish genome reveals adaptations to extreme environments

Antarctic blackfin icefish genome reveals adaptations to extreme environments

A chromosome-level reference genome of the Antarctic blackfin icefish Chaenocephalus aceratus

Intergeneric hybrids inform reproductive isolating barriers in the Antarctic icefish radiation

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