Characterization and In vitro Antioxidant Activity of a Polysaccharide from C ordyceps sobolifera

Abstract

The characteristics and antioxidant activities of Cordyceps sobolifera polysaccharide (CSP) were investigated. Results from high-performance gel filtration chromatography showed that the average molecular weight of CSP was 1.53 × 10 4 kDa, while this polysaccharide was mainly composed of mannose, glucose and galactose in the molar ratio of 1.7:8.9:1 as determined by high-performance liquid chromatography after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone. Fourier-transform infrared spectrum of CSP indicated the typical functional groups of polysaccharide. Antioxidant activity results showed that CSP had reducing power (OD700 was 0.87 at 1.5 mg/mL), and it also exhibited scavenging activities on hydroxyl (·OH scavenging rate was 47.6% at 5 mg/mL) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals (scavenging rate was 55.6% at 1 mg/ mL) in a concentration-dependent manner. These results indicate that CSP has good in vitro antioxidant activity and can potentially be used for functional foods. PRACTICAL APPLICATIONSCordyceps sobolifera is a pricy traditional Chinese herb and it has been used in China for a long time. Polysaccharide extracted from C. sobolifera was characterized, and the antioxidant activities of this polysaccharide were investigated. The result may lay the foundation for the development of antioxidant functional foods from C. sobolifera.

Bioactive Mushroom Polysaccharides: A Review on Monosaccharide Composition, Biosynthesis and Regulation

“…In contrast, intracellular PSs (IPSs) from mycelia contain various polyglucans as well as Glc [ 20 , 21 ]. Compositions of other Cordyceps species were generally similar to those of C. sinensis [ 22 , 23 , 24 , 25 ], although some other monosaccharides, such as Rha, Xyl, Fru and Ara, were present in C. cicadae and C. kyushuensis [ 26 , 27 ]. In Morchella esculenta , another well-known ascomycete, the PSs contain four or more monosaccharides [ 28 , 29 , 30 ].…”

Different Cultivation Environments Affect the Yield, Bacterial Community and Metabolites of Cordyceps cicadae

“…Recent studies have found that C. cicadae contains metabolites with important pharmacological functions. Examples of these include the carbon compound mannitol, which is a diuretic, exhibits antifree-radical activity, and improves cerebral microcirculation and blood flow (Lin et al, 2016;Zhang et al, 2019); polysaccharides, which are also essential carbon compounds, have antioxidant properties and play a role in treating diabetes and improving immune regulation (Lu et al, 2016;Zhang et al, 2018;Wang et al, 2019); adenosine, a nucleoside with anti-inflammatory, neuroprotective and anti-convulsive properties, which has been shown to improve cell viability and prevent and treat neurodegenerative diseases (Latini and Pedata, 2001;Nakav et al, 2008;Olatunji et al, 2016b); and cordycepin, another nucleoside with anti-cancer, immune regulation, and antioxidant functions (Olatunji et al, 2016a;Zhang et al, 2019). Owing to its use as a medicine and tonic, wild C. cicadae has been harvested at unsustainable levels, resulting in a significant population reduction in its natural habitats (Liu et al, 2008).…”

Structural Characterization of Mannoglucan Isolated from Ophiocordyceps sobolifera and Its Antioxidant Activities

“…The PS-T80 average molecular weight is lower than isolated PS from the same species introduced by Lu et al (2016), which is 1.53 × 10 4 kDa. 11 Furthermore, the obtained value is higher than those extracted from Cordyceps sinensis ( C. sinensis ) and Cordyceps militaris ( C. militaris ), ranging from 12.9 to 460 kDa. 30 − 33 …”