Characterization and identification of bacterial pathogens from treated tannery wastewater

Chowdhary, Pankaj; More, Nandkishor; Raj, Abhay; Bharagava, Ram Naresh

doi:10.30918/mri.53.17.016

Cited by 24 publications

(5 citation statements)

References 3 publications

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“…The MIC of Ni was observed to be 8 mM in their study. Another study, conducted by Chowdhary et al [43], stated the MIC of Ni resistant bacterial isolate to be 24 mM, which is in agreement with our results. The results are also similar to the results of Alboghobeish et al [39], who reported the MBC of the Ni-resistant Klebsiella oxytoca species to be 24 mM.…”

Section: Discussionsupporting

confidence: 93%

Evaluation of the Potential Role of Bacillus altitudinis MT422188 in Nickel Bioremediation from Contaminated Industrial Effluents

et al. 2021

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The incessant pervasiveness of heavy metals in the environment is one of the precursory factors of pollution. This research study was endeavored upon to investigate the bioremediation potential of a nickel (Ni)-resistant bacterial isolate, identified as Bacillus altitudinis MT422188, whose optimum growth parameters were demonstrated at pH 7, temperature 32 °C, and 1 mM phosphate. Minimal Inhibitory Concentration (MIC) and EC50 for Ni were observed to be 20 and 11.5 mM, respectively, whereas the cross heavy-metal resistance was discerned as Cu2+ (25 mM) > Zn2+ (15 mM) > Cr6+ (10 mM) > Pb2+ (5 mM) > Co2+ (8 mM) > Cd2+ (3 mM) > Hg2+ (0 mM). Ni biosorption studies by live and heat-killed bacterial cells were suggestive of Ni uptake being facilitated by an ATP-independent efflux system. A pilot-scale study displayed the effective removal of Ni (70 mg/L and 85 mg/L) at 4- and 8-day intervals, respectively. Moreover, chemotaxis and motility assays indicated the role of Ni as a chemoattractant for bacterial cells. The presence of Ni reduced the GR (0.001 ± 0.003 Ug−1FW), POX (0.001 ± 0.001 Ug−1FW), and SOD (0.091 ± 0.003 Ug−1FW) activity, whereas Sodium dodecyl sulphate—Polyacrylamide gel electrophoresis (SDS-PAGE) revealed the presence of metallothionein (60 kDa). Kinetic and isotherm studies suggested a pseudo second-order and Freundlich model to be better fitted for our study. The thermodynamic parameters (∆H° = 3.0436 kJ/mol, ∆S° = 0.0224 kJ/mol/K) suggested the process to be endothermic, spontaneous, and favorable in nature. FTIR analysis elucidated the interaction of hydroxyl and carboxyl groups with Ni. Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray Spectroscopy (EDS) demonstrated changes in the morphological and elemental composition of the bacterial cells, which affirmed their interaction with Ni during biosorption. In summary, our study concludes the efficient role of Bacillus altitudinis MT422188 in removing Ni from polluted industrial effluents.

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Section: Discussionsupporting

confidence: 93%

Evaluation of the Potential Role of Bacillus altitudinis MT422188 in Nickel Bioremediation from Contaminated Industrial Effluents

et al. 2021

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“…However, the results were barely comparable with the toxins of ancient tanneries [ 67 ]. A similar scenario was also recognized for Indian tanneries [ 68 ].…”

Section: Discussionsupporting

confidence: 62%

First Glimpse into the Genomic Characterization of People from the Imperial Roman Community of Casal Bertone (Rome, First–Third Centuries AD)

Angelis

Romboni

Veltre

et al. 2022

Genes

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This paper aims to provide a first glimpse into the genomic characterization of individuals buried in Casal Bertone (Rome, first–third centuries AD) to gain preliminary insight into the genetic makeup of people who lived near a tannery workshop, fullonica. Therefore, we explored the genetic characteristics of individuals who were putatively recruited as fuller workers outside the Roman population. Moreover, we identified the microbial communities associated with humans to detect microbes associated with the unhealthy environment supposed for such a workshop. We examined five individuals from Casal Bertone for ancient DNA analysis through whole-genome sequencing via a shotgun approach. We conducted multiple investigations to unveil the genetic components featured in the samples studied and their associated microbial communities. We generated reliable whole-genome data for three samples surviving the quality controls. The individuals were descendants of people from North African and the Near East, two of the main foci for tannery and dyeing activity in the past. Our evaluation of the microbes associated with the skeletal samples showed microbes growing in soils with waste products used in the tannery process, indicating that people lived, died, and were buried around places where they worked. In that perspective, the results represent the first genomic characterization of fullers from the past. This analysis broadens our knowledge about the presence of multiple ancestries in Imperial Rome, marking a starting point for future data integration as part of interdisciplinary research on human mobility and the bio-cultural characteristics of people employed in dedicated workshops.

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“…Lysinibacillus spp. are bacterial pathogens, while Cloacibacterium are extremotolerant organisms and both have been also reported in this type of effluents (Chowdhary et al 2017, Nascimento et al 2018. Members of the Proteobacteria phylum, such as Brachymonas, Campylobacter, Halomonas, Acinetobacter and Rickettsiella showed similar sequence relative abundances (1-4 %) (Table SV).…”

Section: S1)mentioning

confidence: 61%

“…On the other hand, sequences of pathogenic taxa were also frequently found in other tannery wastewaters. Ricketsiella and Arcobacter genera are pathogens of arthropods and humans, respectively (Chowdhary et al 2017). In addition, Campylobacter is reported as ubiquitous in sewage and it was found that human and animal wastes from abattoirs and animal treatment plants are major sources (Stampi et al 1999).…”

Section: S1)mentioning

confidence: 99%

Characterization of effluents from a tannery industry: a case study of Córdoba province, Argentina

Fernández

González²,

Boarini³

et al. 2022

Rev. Int. Contam. Ambie.

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Tannery effluents are considered to be severe pollutants around the world. The characterization of such effluents is a very important task in order to verify the compliance with legal requirements of the discharge and, if necessary, to optimize their treatment. In this study, we used the effluents from a local tannery (Córdoba, Argentina) that had implemented only a primary treatment. The effluents were periodically collected during one year to assess their physical, chemical, and microbiological characteristics, as well as their toxicity. The physical and chemical characteristics showed variations independently of the sampling time, although, in general, a high nutrient load was the most frequent finding. Moreover, parameters such as chemical oxygen demand, biochemical oxygen demand, total nitrogen, total phosphate, total phenols, Cr(VI) and settleable solids exceeded the limit set by the provincial legislation for the discharge of effluents into surface water bodies. The effluents were also characterized by a high count of bacteria, where pollutant-tolerant and some pathogenic bacteria were found. In sampling 9, Bacteroidetes, Firmicutes and Proteobacteria represented up to 93 % of the total number of sequences obtained, with Paludibacter, Tissierella Soehngenia, Proteocatella and Proteiniclasticum as the most abundant genera. In concordance with these results, all samples showed toxicity according to L. sativa and AMPHITOX bioassays. Altogether, data from this report indicate the need to optimize primary treatment of the effluents, as well as to include complementary treatments in order to reduce the negative environmental impacts of the release of this kind of runoffs.

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Characterization and identification of bacterial pathogens from treated tannery wastewater

Cited by 24 publications

References 3 publications

Evaluation of the Potential Role of Bacillus altitudinis MT422188 in Nickel Bioremediation from Contaminated Industrial Effluents

Evaluation of the Potential Role of Bacillus altitudinis MT422188 in Nickel Bioremediation from Contaminated Industrial Effluents

First Glimpse into the Genomic Characterization of People from the Imperial Roman Community of Casal Bertone (Rome, First–Third Centuries AD)

Characterization of effluents from a tannery industry: a case study of Córdoba province, Argentina

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