Characterization and identification of extracellular polysaccharides-degrading enzymes (epes)-producing marine sediment bacteria

Abstract

The utilization of polysaccharide-degrading enzymes (EPEs) from bacteria has been increasing, therefore many studies are exploring new producers of EPEs. This study aimed to screen and identify the EPEs-producing marine sediment bacteria collected from Panjang Island, Jepara. A total of 11 bacteria were isolated from the sediment sample. Further, seven strains were selected to conduct further analysis, such as biochemical test and screening of enzyme activity. According to the calculation of enzyme activity index (EAI), it was noted that strain with given codes PP.K.15, PP.K.21, PP.K.6, and PP.K.20 demonstrated potential for carrageenase, alginate-lyase, amylase, and agarase. In addition, molecular identification using 16S rRNA gene sequencing discovered that PP.K.6 was identified as Basillus safensis, PP.K.11 as Sinomicrobium oceani, PP.K. 20 as Salinicola zeshunii, PP.K 15 and PP.K. 21 as Micrococcus luteus, and PP.K 22 as Qipengyuania flava.