Characterization and identification of Labisia pumila by multi-steps infrared spectroscopy

Abdullah, Fauziah; Ling, Sui Kiong; Man, Salbiah; Tan, Ai Lee; Tan, Hui‐Meng; Abdullah, Zanariah

doi:10.1016/j.vibspec.2012.06.004

Cited by 8 publications

(8 citation statements)

References 13 publications

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“…The A. paniculata seeds from three germplasm accessions of Malaysia (Accessions 11265, 11341 and 11248, designated as H, P and T, respectively) were collected in July 2011 from Ladang Dua, Universiti Putra Malaysia (UPM). The seeds were germinated at Agro Gene Bank (seed bank) using the sand scarification method . The scarified seeds were then soaked in distilled water before arranging in a Petri dish containing 90 seeds from each accession.…”

Section: Methodsmentioning

confidence: 99%

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Infrared–metabolomics approach in detecting changes in Andrographis paniculata metabolites due to different harvesting ages and times

et al. 2014

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Section: Methodsmentioning

confidence: 99%

“…The seeds were germinated at Agro Gene Bank (seed bank) using the sand scarification method. 11 The scarified seeds were then soaked in distilled water before arranging in a Petri dish containing 90 seeds from each accession. Two filter papers (Whatman No.…”

Section: Plant Materialsmentioning

confidence: 99%

Infrared–metabolomics approach in detecting changes in Andrographis paniculata metabolites due to different harvesting ages and times

et al. 2014

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“…alata using quercetin and myricetin [18]. A combination of HPLC and infrared (IR) spectroscopy methods was implemented to detect marker compounds in L. pumila plants and HMPs [19]. Several bioactive constituent of L. pumila have been identified, mostly by HPLC in the extract, including flavonoids (rutin, apigenin, kaempferol, naringin, and myricetin) and phenolic compound (gallic acid, caffeic acid, and pyrogallol) [8,[20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC

Tarmizi

Wagiran

Salleh

et al. 2021

Plants

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Labisia pumila is a precious herb in Southeast Asia that is traditionally used as a health supplement and has been extensively commercialized due to its claimed therapeutic properties in boosting a healthy female reproductive system. Indigenous people used these plants by boiling the leaves; however, in recent years it has been marketed as powdered or capsuled products. Accordingly, accuracy in determination of the authenticity of these modern herbal products has faced great challenges. Lack of authenticity is a public health risk because incorrectly used herbal species can cause adverse effects. Hence, any measures that may aid product authentication would be beneficial. Given the widespread use of Labisia herbal products, the current study focuses on authenticity testing via an integral approach of DNA barcoding and qualitative analysis using HPLC. This study successfully generated DNA reference barcodes (ITS2 and rbcL) for L.pumila var. alata and pumila. The DNA barcode that was generated was then used to identify species of Labisia pumila in herbal medicinal products, while HPLC was utilized to determine their quality. The findings through the synergistic approach (DNA barcode and HPLC) implemented in this study indicate the importance of both methods in providing the strong evidence required for the identification of true species and to examine the authenticity of such herbal medicinal products.

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“…Recently, many types of phytochemicals have been identified from the leaves, roots and whole plant parts of L. pumila [13][14][15][17][18][19][20][21]. Based on the analytical studies using various detection techniques, it was shown that the plant extract contained significant amount of polyphenols and the sub-group of polyphenols; flavonoids was the major constituents.…”

Section: Introductionmentioning

confidence: 99%

LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY FOR THE DETECTION AND VALIDATION OF QUERCETIN-3-O-RUTINOSIDE AND MYRICETIN FROM FRACTIONATED Labisia pumila var. alata

2018

MJAS

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Quercetin-3-O-rutinoside and myricetin was reported to be important secondary metabolites in Labisia pumila (L. pumila). The reliability of the instrument, its sensitivity and methodology are the key in the analysis of a specific metabolite and liquid chromatography tandem mass spectrometry (LC-MS/MS) provides better accuracy and faster separation in detecting of such chemical compound. Therefore, this study aims to detect and validate the presence of quercetin-3-O-rutinoside and myricetin from fractionated extract of L. pumila (40% MeOH: water). The plant material was fractionated using octadecyl (C 18 ) silica solid phase and eluent with a ratio of 40% MeOH: water. The separation of compounds was carried out using a C 18 reversed phase column (Acquity, 150mm × 4.6mm × 1.7 µm). Negative ionization was utilized to fragment the precursor ion of m/z 609 for quercetin-3-O-rutinoside and m/z 317 for myricetin. Three fragments ions; m/z 447, 463, 301 was recognized as quercetin-3-Orutinoside product ions within 3.3 minutes. Meanwhile, m/z 271,137 and 151 represented characteristics ions for myricetin were recognized within 3.9 minutes. The concentration of quercetin-3-O-rutinoside and myricetin was 0.007 mg/g and 0.009 mg/g respectively. The linearity was found between 0.991-0.998. Detection based on (limit of detection) LOD and (limit of quantification) LOQ were found in the ranged of 0.03-0.04 µg/mL and 0.11-0.13 µg/mL, respectively. Both compounds showed good recovery of above 87%. Intra-day and inter-day (RSD) study was in the acceptable range (below 10%). Based on the result of this study, the LC-MS/MS was shown to be the best selective, fast and sensitive method to determine quercetin-3-O-rutinoside and myricetin in the plant fraction. These findings could be used as a guideline for the detection of the compounds and suitable for quality controls of L. pumila this and another medicinal herb containing quercetin-3-O-rutinoside and myricetin. AbstrakKuersetin-3-O-rutinosida dan mirisetin telah dilaporkan sebagai metabolit sekunder yang penting dalam Labisia pumila (L. pumila). Kebolehpercayaan instrumen, kepekaan dan kaedah pembangunan menjadi aspek penting dalam analisis yang disasarkan kepada metabolit tertentu dan kromatografi cecair spektrometri jisim tandem (KC-SJ/SJ) menyediakan pengasingan lebih cepat dan tepat bagi pengesanan sebatian kimia. Oleh itu, kajian ini adalah bertujuan untuk mengesan dan menentusahkan kehadiran kuersetin-3-O-rutinosida dan mirisetin daripada pemeringkatan L. pumila (40% metanol:air). Bahan tumbuhan ini ISSN 1394 -2506 Norliza et al: LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY FOR THE DETECTION AND VALIDATION OF QUERCETIN-3-O-RUTINOSIDE AND MYRICETIN FROM FRACTIONATED Labisia pumila var. alata 818 diperingkatkan menggunakan fasa pepejal oktadesil silika (C 18 ) dengan kadar 40% metanol: air. Pengasingan komponen dicapai menggunakan fasa turus berbalik C 18 (Acquity, 150 mm × 4.6 mm × 1.7 µm). Pengionan negatif telah digunakan untuk mengasingkan pencetus ion m/z 609 untuk kuerse...

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Characterization and identification of Labisia pumila by multi-steps infrared spectroscopy

Cited by 8 publications

References 13 publications

Infrared–metabolomics approach in detecting changes in Andrographis paniculata metabolites due to different harvesting ages and times

Infrared–metabolomics approach in detecting changes in Andrographis paniculata metabolites due to different harvesting ages and times

Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC

LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY FOR THE DETECTION AND VALIDATION OF QUERCETIN-3-O-RUTINOSIDE AND MYRICETIN FROM FRACTIONATED Labisia pumila var. alata

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