Characterization and Identification of Organic Selenium-enriched Bacteria Isolated from Rumen Fluid and Hot Spring Water

Dalia, A. M.; Loh, Teck Chwen; Sazili, Awis Qurni; Jahromi, Mohammad Faseleh; Samsudin, Anjas Asmara

doi:10.4014/mbl.1712.12010

Cited by 9 publications

(6 citation statements)

References 31 publications

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“…On the other hand, Se supplementation in the form of probiotics bacteria and bacterial extract has received more consideration in the last years [ 8 ], a wide range of selenium enriched bacteria were identified as organic source of selenium since they are able to uptake inorganic Se and accumulate it in their cells in the form of selenoprotein [ 9 ]. Previously, we stated that some organic Se-enriched bacteria identified as Enterobacter cloacae , (ADS1), Klebsiella pneumoniae (ADS2), and Stenotrophomonas maltophilia (ADS18), were showed high ability to accumulate around 50% of their absorbed Se as selenoproteins in their cells, and their extracted selenoprotein showed high Se concentrations with moderate antioxidant properties, which can be considered as a potential source of organic Se [ 10 , 11 ]. Mineral functional food showed a beneficial effect on human health and nutritional status, it decreasing the minerals deficiency issues and prevent humans from various diseases.…”

Section: Introductionmentioning

confidence: 99%

Effect of Microbiota-Selenoprotein on Meat Selenium Content and Meat Quality of Broiler Chickens

Mohamed

Sazili

Loh

et al. 2020

Animals

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Selenium (Se) is able to transform from inorganic to organic forms via many bacterial species. This feature is being considered for delivering more bioavailable selenium compounds such as selenocysteine and selenomethionine for human and animal diet. This study investigated the effects of bacterial selenoprotein versus inorganic Se on the carcass characteristics, breast meat selenium content, antioxidant status, and meat quality of broiler chickens. One hundred and eighty chicks were randomly allotted to five treatments of a basal diet supplemented with no Se, sodium selenite, Enterobacter cloacae Selenium (ADS1-Se), Klebsiella pneumoniae-Selenium (ADS2-Se), and Stenotrophomonas maltophilia-Selenium (ADS18-Se). The results showed that bacterial selenoprotein has the ability to deposit more Se in the breast meat compared to sodium selenite. Both Se sources reduced breast meat drip loss, cooking loss, shear force, and 2-thiobarbituric acid reactive substances (TBARS) significantly. It also increased total antioxidant (TAC) and glutathione peroxidase (GSH-Px) in comparison with the negative control. The highest activity of (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) was found in bacterial selenoprotein. In conclusion, bacterial selenoprotein is more efficient than sodium selenite in increasing the breast meat Se deposition and oxidative capacity of broiler chickens. Therefore, it can be effectively used to produce Se-rich meat as a functional food.

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Section: Introductionmentioning

confidence: 99%

Effect of Microbiota-Selenoprotein on Meat Selenium Content and Meat Quality of Broiler Chickens

Mohamed

Sazili

Loh

et al. 2020

Animals

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“…The detection limit was 0.8 ng/mL, and the quantification limit was 4.0 nM, with a relative standard deviation (precision of repeated injections) of 2.2%. The procedure for bacterial selenoproteins from S. maltophilia production is described in the previous studies [ 27 ]. The hens were fed 120 g of feed a day per hen, and unlimited access to water.…”

Section: Methodsmentioning

confidence: 99%

“…There is also a new organic Se-enriched dietary source derived from bacteria ( Stenotrophomonas maltophilia (ADS18)) [ 27 ], which may provide health-related benefits to humans through animal products. To our knowledge, no research has been done on its (Se-enriched bacterial proteins of ADS18) use in layers.…”

Section: Introductionmentioning

confidence: 99%

Effect of Sodium Selenite, Selenium Yeast, and Bacterial Enriched Protein on Chicken Egg Yolk Color, Antioxidant Profiles, and Oxidative Stability

Muhammad

Mohamed

Loh

et al. 2021

Foods

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The chicken egg is one of nature’s flawlessly preserved biological products, recognized as an excellent source of nutrients for humans. Selenium (Se) is an essential micro-element that plays a key role in biological processes. Organic selenium can be produced biologically by the microbial reduction of inorganic Se (sodium selenite). Therefore, the possibility of integrating Se enriched bacteria as a supplement in poultry feed can provide an interesting source of organic Se, thereby offering health-related advantages to humans. In this study, bacterial selenoproteins from Stenotrophomonas maltophilia was used as a dietary supplement with other Se sources in Lohman brown Classic laying hens to study the egg yolk color, egg yolk and breast antioxidant profile, oxidative stability, and storage effect for fresh and stored egg yolk at 4 ± 2 °C for 14-days. The results showed that dietary Se supplementation significantly (p < 0.05) improved egg yolk color, the antioxidant profile of egg yolk, and breast meat (total carotenoid and phenol content). When the Se treated groups were compared to control groups, there was a significant (p < 0.05) decrease in total cholesterol in fresh and stored egg yolk and breast muscle. In hens that were fed ADS18-Se, the primary oxidation products (MDA) concentrations in the eggs, breast, and thigh muscle, and plasma were significantly (p < 0.05) lower. However, the MDA content increased (p < 0.05) with an extended storage time in egg yolk. In comparison to inorganic Se and basal diets, egg yolk from hens fed organic Se remained fresh for two weeks. The egg yolk color, antioxidant profile, and oxidative status of egg yolk and tissue improve with dietary Se organic supplementation (ADS18 > Se-Yeast). The source of supplemented organic Se is critical for egg enrichment and antioxidant properties. As a result, ‘‘functional eggs’’ enriched with organic Se becomes possible to produce.

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“…The bacterial Se used in the study were sourced from Stenotrophomonas maltophilia, ADS18 (ADS18), which was obtained by isolating the bacteria from Selayang hot spring in Selangor (Dalia et al, 2017a). Following the procedure described by the author, the stock culture was cultivated in the Laboratory of Microbiology, Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM).…”

Section: Bacterial Se Ads18 Preparationmentioning

confidence: 99%

Supplementation of different sources of selenium on laying performance, egg quality traits, and shell calcification expressions in 50 weeks Lohmann Brown layer hen reproductive tract

Hemly,

Zainuddin,

Muhammad

et al. 2023

VIS

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Massive consumption and exportation of eggs in Malaysia are possible by maintaining and improving layer productive performances and egg quality through antioxidant supplementation such as selenium. The bioavailability of organic selenium in tissues resulted in a preference for organic selenium in poultry diets. The present study assesses the effects of feeding 50 weeks layer hens with sodium selenite, selenised yeast, and Stenotrophomonas maltophilia, ADS18 as a source of selenium and vitamin E on laying integrity, egg qualities, and reproductive gene expressions. The four different treatments (diets) used in the experiment were tagged as follows: Control: a basal diet containing 100 mg/kg vitamin E, SS: basal diet plus 0.3 mg/kg sodium selenite, Se-yeast: basal diet plus 0.3 mg/kg selenised yeast, and VADS18: basal diet plus 0.3 mg/kg ADS18. Productive performances were analysed throughout a 90-days of production. Eggs were collected biweekly, and egg qualities were measured. The uterine and magnum tissues were examined for the genes ovocleidin and ovocalyxin; OCX32, OCX36, OC17 and OC116. The results showed that Se-yeast significantly (P < 0.05) increased laying integrity, while VADS18 supplementation significantly (P < 0.05) increased egg weight. Supplementation of different selenium sources significantly (P < 0.05) improved egg production and quality. Additionally, uterine gene expression was significantly (P < 0.05) increased by VADS18 supplementation. Therefore, adding organic selenium to the diet maintained various laying performance indicators, egg qualities, and upregulated gene expression. Further evaluations are required to determine the optimum inclusion level of ADS18 poultry.

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Characterization and Identification of Organic Selenium-enriched Bacteria Isolated from Rumen Fluid and Hot Spring Water

Cited by 9 publications

References 31 publications

Effect of Microbiota-Selenoprotein on Meat Selenium Content and Meat Quality of Broiler Chickens

Effect of Microbiota-Selenoprotein on Meat Selenium Content and Meat Quality of Broiler Chickens

Effect of Sodium Selenite, Selenium Yeast, and Bacterial Enriched Protein on Chicken Egg Yolk Color, Antioxidant Profiles, and Oxidative Stability

Supplementation of different sources of selenium on laying performance, egg quality traits, and shell calcification expressions in 50 weeks Lohmann Brown layer hen reproductive tract

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