Characterization and identification of pradimicin analogs from Actinomadura hibisca using liquid chromatography–tandem mass spectrometry

Park, Je Won; Park, Sung Ryeol; Han, Ah Reum; Ban, Yeon Hee; Yoo, Young Je; Kim, Eunji; Kim, Beom Seok; Sohng, Jae Kyung; Yoon, Yeo Joon

doi:10.1016/j.chroma.2011.02.034

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…Strains closely related to A. rudentiformis were isolated in this study, which exhibits antibiosis against Entorococcus faecium and some Mycobacterium pathogenic species . Additionally, A. hisbisca produces polyenoles, called pradamicin, that have antifungal and antiviral activity .…”

Section: Discussionmentioning

confidence: 99%

Paratrechina longicornis ants in a tropical dry forest harbor specific Actinobacteria diversity

Reyes

Cafaro

2014

J. Basic Microbiol.

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The diversity of Actinobacteria associated with Paratrechina longicornis, an ant species that prefers a high protein diet, in a subtropical dry forest (Guánica, Puerto Rico) was determined by culture methods and by 16S rDNA clone libraries. The results of both methodologies were integrated to obtain a broader view of the diversity. Streptomyces, Actinomadura, Nocardia, Ornithinimicrobium, Tsukamurella, Brevibacterium, Saccharopolyspora, Nocardioides, Microbacterium, Leifsonia, Pseudonocardia, Corynebacterium, Geodermatophilus, Amycolatopsis, and Nonomuraea were found associated with the ants. The genera Streptomyces and Actinomadura were the most abundant. Also, the diversity of Actinobacteria associated with the soil surrounding the nest was determined using 16S rDNA clone libraries. In total, 27 genera of Actinobacteria were associated with the nest soils. A dominant genus was not observed in any of the soil samples. We compared statistically the Actinobacteria communities among P. longicornis nests and each nest with its surrounding soil using the clone libraries data. We established that the communities associated with the ants were consistent and significantly different from those found in the soil in which the ants live.

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Section: Discussionmentioning

confidence: 99%

Paratrechina longicornis ants in a tropical dry forest harbor specific Actinobacteria diversity

Reyes

Cafaro

2014

J. Basic Microbiol.

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Review of sample preparation strategies for MS-based metabolomic studies in industrial biotechnology

Causon

2016

Analytica Chimica Acta

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Genetic evidence for the involvement of glycosyltransferase PdmQ and PdmS in biosynthesis of pradimicin from Actinomadura hibisca

Jha

Paudel

Dhakal

et al. 2015

Microbiological Research

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Pradimicins are potent antifungal antibiotics with effective inhibitory effects against HIV-1. Pradimicin A consists of an unusual dihydrobenzo[α]naphthacenequinone aglycone substituted with a combination of D-alanine and two sugar moieties. Detailed genetic studies revealed most steps in pradimicin A biosynthesis, but the glycosylation mechanism remained inconclusive. The biosynthetic gene cluster of pradimicin A contains two putative glycosyltransferases, pdmQ and pdmS. However, the exact involvement of each gene in biosynthesis and the particular steps required for precise structural modification was unknown. In this study, the exact role of each gene was evaluated by insertional inactivation and complementation studies. Analysis of the metabolite from both of the disruption mutants revealed abolishment of pradimicin A and complementation resulted in the recovery of production. After deletion of pdmQ, pradimicin B was found to accumulate, whereas deletion of pdmS resulted in the accumulation of aglycone of pradimicin. Together, these results suggest that pdmS is responsible for the attachment of thomosamine to form pradimicin B which in turn is glycosylated by pdmQ to form pradimicin A. These results allowed us to deduce the exact order of terminal tailoring by glycosylation and provided insight into the mechanism of pradimicin A biosynthesis.

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Characterization and identification of pradimicin analogs from Actinomadura hibisca using liquid chromatography–tandem mass spectrometry

Cited by 3 publications

References 21 publications

Paratrechina longicornis ants in a tropical dry forest harbor specific Actinobacteria diversity

Paratrechina longicornis ants in a tropical dry forest harbor specific Actinobacteria diversity

Review of sample preparation strategies for MS-based metabolomic studies in industrial biotechnology

Genetic evidence for the involvement of glycosyltransferase PdmQ and PdmS in biosynthesis of pradimicin from Actinomadura hibisca

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