Characterization and identification of three thermophilic Bacillus strain isolated from Domas Crater, Mt. Tangkuban Perahu, Indonesia

Abstract

Abstract. Safitri R, Kusumawardhani PD, Annisa, Partasasmita R, Asharina S, Maskoen AM. 2020. Characterization and identification of three thermophilic Bacillus strains isolated from Domas Crater, Mt. Tangkuban Perahu, Indonesia. Biodiversitas 21: 3444-3453. A community of thermophiles within the hot spring of Domas Crater, Mount Tangkuban West Java, has been cultivated and identified based on the 16S ribosomal RNA gene sequence. The hot spring has a temperature of 45°C-76 °C, pH 1-2, the isolate has been cultivated in Thermus medium at a temperature of 70 and pH 6. The three isolated strains were L61A, TS61A, and D41A identified based on the 16S ribosomal RNA gene sequence refer to the GenBank database and all of them belong to the Phylum Firmicutes, which are clustered within the taxonomic groups of Bacillus, the Bacillus, and Geobacillus genera. Nucleotide sequences were compared for homology with BLASTN search at the NCBI Web-site revealed that strains (L61A) had 99.93% similarity with Geobacillus uzenensis. TS61A strain has a 99.24% similarity with Bacillus wiedmannii. Whereas D41A has 99.79% similarity with Bacillus paramycoides. The characterization also includes the phenotypic and biochemical of strains. The bacillus genera were known as a source of thermostable enzymes, plant growth-promoting, probiotics, decomposers of various polysaccharide materials, and bioremediation.

Identification, characterization and hydrolase producing performance of thermophilic bacteria: geothermal hot springs in the Eastern and Southeastern Anatolia Regions of Turkey

“…It is known that thermophilic microorganisms have a diversity that includes both bacteria and archaea, and the most frequently isolated thermophilic strains have also been reported to belong to the Bacillus genus (Abootalebi et al 2020 ; Ladeira et al 2015 ; Shen et al 2021 ; Verma et al 2018 ; Wang et al 2006 ). The presence of thermophilic Bacilli has been investigated in hot springs and compost sources around the world (Yanmis et al 2015 ), such as those in India (Verma et al 2018 ), Iran (Abootalebi et al 2020 ), Indonesia (Safitri et al 2020 ), Jordan (Mohammad et al 2017 ), Faisalabad (Saleh et al 2020 ), Tunisia (Salem et al 2020 ), Brazil (Bernardo et al 2020 ), Egypt (Saeed et al 2020 ), Malaysia (Msarah et al 2020 ), Vietnam (Dang et al 2018 ), Saudi Arabia (Al-Johani et al 2016 ), South Africa (Tsotetsi et al 2020 ) and Japan (Akita et al 2017 ).…”

Molecular characterization of indigenous microbes and its potential as a biological control agent of Fusarium stem rot disease (Fusarium verticillioides) on maize

“…B. cereus has short rod-shaped cells to single rods with a single arrangement (Figure S3c, S3f). Safitri et al. (2020) described the characteristics of B. cereus bacteria, i.e white, circular shape, short bacilli with all edges, and convex elevation.…”

Ecological versatility and biotechnological promise: Comprehensive characterization of the isolated thermophilic Bacillus strains

“…This finding contrasts with the research conducted by [ 19 , 20 , 21 ], which found a more consistent preference for oxygen among the thermophilic bacteria studied. These differences suggest that there may be variations in environmental adaptation among different strains or species of bacteria [ 18 , 19 , 20 ].…”