Characterization and in vitro evaluation of a vaginal gel containing Lactobacillus crispatus for the prevention of gonorrhea

Gnaman, K.C. N'Guessan; Bouttier, Sylvie; Yeo, A.; Any-Grah, A.A.S. Aka; Geiger, Stefania; Huang, Nicolas; Nicolas, Valérie; Villebrun, S.; Faye-Ketté, H.; Ponchel, Gilles; Akpadza, Koffi; Agnely, Florence

doi:10.1016/j.ijpharm.2020.119733

Cited by 15 publications

(4 citation statements)

References 63 publications

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“…It was observed that the probiotic associated in the formulation remained viable for a longer time when stored in the refrigerator, which is in agreement with the study that evaluated the viability of lactobacilli strains when stored at a low temperature [ 40 ]. Similar results were also observed in a vaginal gel formulation containing Lactobacillus crispatus ATCC 33197 for the prevention of gonorrhea, which confirmed its longer viability when stored at 4 °C, although its viability dropped dramatically after two weeks [ 41 ].…”

Section: Discussionsupporting

confidence: 68%

Development and Characterization of Hydroxyethyl Cellulose-Based Gels Containing Lactobacilli Strains: Evaluation of Antimicrobial Effects in In Vitro and Ex Vivo Models

et al. 2023

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This study aimed to develop a hydroxyethyl cellulose-based topical formulation containing probiotics and to evaluate its antimicrobial action using in vivo and ex vivo models. Initially, the antagonistic effects of Lacticaseibacillus rhamnosus ATCC 10863, Limosilactobacillus fermentum ATCC 23271, Lactiplantibacillus plantarum ATCC 8014 and Lactiplantibacillus plantarum LP-G18-A11 were analyzed against Enterococcus faecalis ATCC 29212, Klebsiella pneumoniae ATCC 700603, Staphylococcus aureus ATCC 27853 and Pseudomonas aeruginosa ATCC 2785. The best action was seen for L. plantarum LP-G18-A11, which presented high inhibition against S. aureus and P. aeruginosa. Then, lactobacilli strains were incorporated into hydroxyethyl cellulose-based gels (natrosol); however, only the LP-G18-A11-incorporated gels (5% and 3%) showed antimicrobial effects. The LP-G18-A11 gel (5%) maintained its antimicrobial effects and viability up to 14 and 90 days at 25 °C and 4 °C, respectively. In the ex vivo assay using porcine skin, the LP-G18-A11 gel (5%) significantly reduced the skin loads of S. aureus and P. aeruginosa after 24 h, while only P. aeruginosa was reduced after 72 h. Moreover, the LP-G18-A11 gel (5%) showed stability in the preliminary and accelerated assays. Taken together, the results show the antimicrobial potential of L. plantarum LP-G18-A11, which may be applied in the development of new dressings for the treatment of infected wounds.

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Section: Discussionsupporting

confidence: 68%

Development and Characterization of Hydroxyethyl Cellulose-Based Gels Containing Lactobacilli Strains: Evaluation of Antimicrobial Effects in In Vitro and Ex Vivo Models

et al. 2023

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“…Survival of pathogens in three different mimicked vaginal environments (pH 4.2; 5.5 and 6.5) was investigated in the presence of Fitostimoline® Septagel. Keeping the proportion of 5 g of Septagel to 0.75 mL of SVF [24], for each pH, 100 g of Fitostimoline® Septagel…”

Section: Effect In Mimic Vaginal Modelmentioning

confidence: 99%

“…In our trial we applied 5 g of gel in the following vaginal conditions: temperature 37°C and 0.75 mL of female genital tract secretion,simulated vaginal fluid (SVF) -at a pH 4.2 (normal conditions), and at a pH 5.5 and pH 6.5 mimicking the infection state [24].…”

Section: Physicochemical Evaluation Of Fitostimoline® Septagel In Mim...mentioning

confidence: 99%

In Vitro Characterization of a Novel Medical Device in Simulated Vaginal Environment

Schiavo

2022

AJBSR

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Bacterial vaginosis, aerobic vaginitis and candidiasis are the most common infections in women in fertile age, characterized by an imbalanced vaginal microbiota population and vaginal discharge. The therapeutic approach could vary from antibiotics to antiseptics with the risk of developing resistance. For this reason, a novel hydrogel formulation, Fitostimoline® Septagel, has been developed for the local treatment of irritative-inflammatory-dystrophic states of the vaginal area. The aim of this study was to evaluate, by an in vitro assay, the efficacy of Fitostimoline® Septagel on the most common vaginitis and vaginosis pathogen.Agar spot overlay test and the survival of Streptococcus agalactiae, Escherichia coli and Candida albicans pathogens were detected in a simulated vaginal model. Furthermore, to determine the physical properties of Fitostimoline® Septagel, pH, viscosity and the swelling index of the gel were analyzed.This study demonstrates the in vitro effectiveness of Fitostimoline® Septagel as an adjuvant treatment of vaginitis and vaginosis. The results show that Fitostimoline® Septagel has a positive effect on the pH of the environment in which it is introduced, it maintains its hydrogel properties for the entire duration of the application, guaranteeing adhesion to the place of application and counteracting the proliferation of pathogens of bacterial and fungal origin.

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“…When P407 is administered via injection, it has the advantages of minimal trauma, delayed drug release, reduced administration times, low toxicity and immunogenicity. Poloxamer thermosensitive gels are widely used in drug delivery systems, such as nasal, eye, vaginal, periodontal, skin, tumoral, joint and other tissues [ 20 , 21 , 22 , 23 ], due to their rapid gelation, suitable viscoelasticity and special three-dimensional mesh structure.…”

Section: Introductionmentioning

confidence: 99%

Preparation, Properties and Therapeutic Effect of a TPL Nanoparticle Thermosensitive Gel for Intra-Articular Injection

et al. 2023

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Most injectable preparations for the articular cavity are solution-type preparations that are frequently administered because of rapid elimination. In this study, triptolide (TPL), an effective ingredient in the treatment of rheumatoid arthritis (RA), was prepared in the form of a nanoparticle thermosensitive gel (TPL-NS-Gel). The particle size distribution and gel structure were investigated by TEM, laser particle size analysis and laser capture microdissection. The effect of the nanoparticle carrier material PLGA on the phase transition temperature was investigated by 1H variable temperature NMR and DSC. The tissue distribution, pharmacokinetic behavior, four inflammatory factors and therapeutic effect were determined in a rat RA model. The results suggested that PLGA increased the gel phase transition temperature. The drug concentration of the TPL-NS-Gel group in joint tissues was higher than that in other tissues at different time points, and the retention time was longer than that of the TPL-NS group. After 24 days of administration, TPL-NS-Gel significantly improved the joint swelling and stiffness of the rat models, and the improvement degree was better than that of the TPL-NS group. TPL-NS-Gel significantly decreased the levels of hs-CRP, IL-1, IL-6 and TNF-α in serum and joint fluid. There was a significant difference between the TPL-NS-Gel and TPL-NS groups on Day 24 (p < 0.05). Pathological section results showed that inflammatory cell infiltration was lower in the TPL-NS-Gel group, and no other obvious histological changes were observed. Upon articular injection, the TPL-NS-Gel prolonged drug release, reduced the drug concentration outside the articular tissue and improved the therapeutic effect in a rat RA model. The TPL-NS-Gel can be used as a new type of sustained-release preparation for articular injection.

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Characterization and in vitro evaluation of a vaginal gel containing Lactobacillus crispatus for the prevention of gonorrhea

Cited by 15 publications

References 63 publications

Development and Characterization of Hydroxyethyl Cellulose-Based Gels Containing Lactobacilli Strains: Evaluation of Antimicrobial Effects in In Vitro and Ex Vivo Models

Development and Characterization of Hydroxyethyl Cellulose-Based Gels Containing Lactobacilli Strains: Evaluation of Antimicrobial Effects in In Vitro and Ex Vivo Models

In Vitro Characterization of a Novel Medical Device in Simulated Vaginal Environment

Preparation, Properties and Therapeutic Effect of a TPL Nanoparticle Thermosensitive Gel for Intra-Articular Injection

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