Characterization and in-vitro screening of probiotic potential of novel Weissella confusa strain GCC_19R1 isolated from fermented sour rice

Nath, Soumitra; Roy, Monisha; Sikidar, Jibalok; Deb, Bibhas; Sharma, Indu; Guha, Anupam

doi:10.1016/j.crbiot.2021.04.001

Cited by 28 publications

(5 citation statements)

References 69 publications

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“…Fermented sour rice is another traditional food of the Indian subcontinent which is believed to have therapeutic and prophylactic applications against various disorders. The probiotic candidate, Weissella confusa strain GCC 19R1 was found to be the predominant fermentative bacteria in this product [73]. Interestingly, a probiotic-fermented rice tablet has also been tested recently.…”

Section: Rice-based Productsmentioning

confidence: 98%

Probiotic Delivery through Non-Dairy Plant-Based Food Matrices

et al. 2021

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Probiotics are live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. Traditionally, dairy products are the major and most popular probiotic carriers. At present, there is a growing demand for non-dairy probiotic products. Both fermented and non-fermented non-dairy plant-based food products are becoming highly appealing to both dairy and non-dairy consumers worldwide. Non-dairy plant-based food matrices such as fruits, vegetables, plant-based milk, cereals, and legumes have been used successfully in producing probiotic products with the minimum recommended viable probiotic numbers at the time of consumption. However, due to the exclusion of dairy, whether these food matrices can enhance the functional properties of probiotics such as gastrointestinal survival and immune-enhancing effects needs a thorough investigation. Hence, this review focuses on some of the popular non-dairy plant-based probiotic food products and their microbiological quality characteristics in terms of maintaining probiotic viability during product storage. Their gastrointestinal tolerance in these products, other functional properties, and product qualities have also been briefly discussed.

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Section: Rice-based Productsmentioning

confidence: 98%

Probiotic Delivery through Non-Dairy Plant-Based Food Matrices

et al. 2021

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“…The sugar fermentation patterns of the LAB isolates were tested for various sugars, such as glucose, fructose, arabinose, sucrose, sorbitol and xylose [13]. Salt resistance of isolates was tested per [14] with modified NaCl levels (4.5%, 6.5%, 8.5% and 10.5%).…”

Section: Morphological and Physiobiochemical Characterization Of Lab ...mentioning

confidence: 99%

Quantification of Antimicrobial Compounds Produced by Lactic Acid Bacteria Isolated from Pulses

Gouthami,

Ramalakshmi,

Balakrishnan

et al. 2023

IJPSS

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In this study, 11 LAB isolates were isolated from the pulses (green gram and black gram) were tested for biochemical characteristics (catalase, MR-VP, citrate utilization, nitrate reduction) based on these tests four isolates were evaluated for their antimicrobial activity against three prominent pathogens: Escherichia coli, Pseudomonas aeruginosa, and Aspergillus flavus NRRL3357 under different pH 9 (5.5, 6.5, 7.5 and 8.5) and temperature (17ºC, 27ºC, and 40ºC). Among the four isolates, one isolate LB4 exhibited notable antimicrobial efficacy against all three pathogens under pH (6.5) 8.1± 0.6 mm, 8.3 ± 0.5 mm, and 6.6 ± 0.8 mm respectively. Optimum antimicrobial activity showed at temperature 27º against same pathogens the zone of inhibition mean values 10± 0.9 mm, 5.0± 0.6 mm, 8.3± 1.2 mm respectively. LB4 isolate was tested for quantification of key metabolites after three days of incubation, lactic acid, acetic acid, and hydrogen peroxide. Lactic acid (4.6 g/l) and acetic acid (4.8 g/l) were consistently produced in higher amounts, while hydrogen peroxide production remained relatively low (0.58 g/l) by the LB4 isolate. The results indicated the intricate relationship between antimicrobial activity, environmental factors, and metabolite production, emphasizing the promising role of this isolate in combating microbial infections under diverse conditions.

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“…Probiotic bacteria also show bactericidal or bacteriostatic activity though the synthesis of small ribosomal peptides called bacteriocins [84]. Only six purified bacteriocins have been reported to be produced by W. cibaria, W. paramesenteroides, and W. hellenica [85]. Srionnual et al [86] were the first to report a unique bacteriocin called 'weissellicin 110' produced by the W. cibaria strain, 110 (isolated from a Thai fermented fish product).…”

Section: Antimicrobial Potentialmentioning

confidence: 99%

“…No inhibitory effect on the growth of S. aureus was noted. Yet another strain, W. confusa GCC_19R1, was found to have antibacterial activity against the Gram-negative bacteria Stenotrophomonas maltophilia, Acinetobacter johnsonii, Achromobacter spanius, and Cedecea davisae [85]. The bacteriocin synthesized by the W. confusa strain, PL9001, exhibits antagonistic activity against the pathogen Helicobacter pylori and can be used to treat H. pylori-induced gastritis and gastric ulcers [97,98].…”

Section: Antimicrobial Potentialmentioning

confidence: 99%

“…The vast diversity of this genus and the deficiency of scientific literature could be possible reasons for not being granted the GRAS status by the FDA (Food and Drug Administration) or by the EFSA (European Food Safety Authority). The genus is also often classified as opportunistic pathogens, which seems to limit its application as potentially beneficial probiotics in the food industry despite being potentially powerful starters for food fermentation [85,137].…”

Section: Starter Culturementioning

confidence: 99%

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The Weissella Genus: Clinically Treatable Bacteria with Antimicrobial/Probiotic Effects on Inflammation and Cancer

Ahmed

Singh

et al. 2022

Microorganisms

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Weissella is a genus earlier considered a member of the family Leuconostocaceae, which was reclassified into the family Lactobacillaceae in 1993. Recently, there have been studies emphasizing the probiotic and anti-inflammatory potential of various species of Weissella, of which W. confusa and W. cibaria are the most representative. Other species within this genus include: W. paramesenteroides, W. viridescens, W. halotolerans, W. minor, W. kandleri, W. soli, W. ghanensis, W. hellenica, W. thailandensis, W. fabalis, W. cryptocerci, W. koreensis, W. beninensis, W. fabaria, W. oryzae, W. ceti, W. uvarum, W. bombi, W. sagaensis, W. kimchi, W. muntiaci, W. jogaejeotgali, W. coleopterorum, W. hanii, W. salipiscis, and W. diestrammenae. Weissella confusa, W. paramesenteroides, W. koreensis, and W. cibaria are among the few species that have been isolated from human samples, although the identification of these and other species is possible using metagenomics, as we have shown for inflammatory bowel disease (IBD) and healthy controls. We were able to isolate Weissella in gut-associated bacteria (post 24 h food deprivation and laxatives). Other sources of isolation include fermented food, soil, and skin/gut/saliva of insects/animals. With the potential for hospital and industrial applications, there is a concern about possible infections. Herein, we present the current applications of Weissella on its antimicrobial and anti-inflammatory mechanistic effects, the predisposing factors (e.g., vancomycin) for pathogenicity in humans, and the antimicrobials used in patients. To address the medical concerns, we examined 28 case reports focused on W. confusa and found that 78.5% of infections were bacteremia (of which 7 were fatal; 1 for lack of treatment), 8 were associated with underlying malignancies, and 8 with gastrointestinal procedures/diseases of which 2 were Crohn’s disease patients. In cases of a successful resolution, commonly administered antibiotics included: cephalosporin, ampicillin, piperacillin-tazobactam, and daptomycin. Despite reports of Weissella-related infections, the evolving mechanistic findings suggest that Weissella are clinically treatable bacteria with emerging antimicrobial and probiotic benefits ranging from oral health, skin care, obesity, and inflammatory diseases to cancer.

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Characterization and in-vitro screening of probiotic potential of novel Weissella confusa strain GCC_19R1 isolated from fermented sour rice

Cited by 28 publications

References 69 publications

Probiotic Delivery through Non-Dairy Plant-Based Food Matrices

Probiotic Delivery through Non-Dairy Plant-Based Food Matrices

Quantification of Antimicrobial Compounds Produced by Lactic Acid Bacteria Isolated from Pulses

The Weissella Genus: Clinically Treatable Bacteria with Antimicrobial/Probiotic Effects on Inflammation and Cancer

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