Characterization and Isolation of the Major Biologically Active Metabolites Isolated from Ficus retusa and Their Synergistic Effect with Tetracycline against Certain Pathogenic-Resistant Bacteria

Abdulwahab, Alqahtani; Marrez, Diaa A.; Aleraky, Mohamed; Fagir, Nada A.; Alqahtani, Omaish; Osman, Samir; Raey, Mohamed A. El; Attia, Gouda H.

doi:10.3390/ph15121473

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…There are reports on the antimicrobial activity of extracts (containing a flavonoids mixture) obtained from Hedera helix (gallic acid and CT) [128] and Teucrium (including CT) [129], which obtained similar results-slight activity against K. pneumoniae. In the work of Alqahtani et al ( 2022), the synergistic effect of CT (extract from Ficus retusa) with tetracycline against E. coli, P. aeruginosa, and S. aureus and the additive against K. pneumoniae (the MIC value for CT = 2.33 mg•mL −1 ) was presented [130]. In an in vivo model of rat skin infection, the antimicrobial activity of extracts from the fruit of Parkia clappertoniana (con-taining quercetin and CT) and their effects on wound healing were investigated.…”

Section: Klebsiella Pneumoniaementioning

confidence: 99%

Bioactive Compounds from Plant Origin as Natural Antimicrobial Agents for the Treatment of Wound Infections

Pacyga,

Topola

et al. 2024

IJMS

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The rising prevalence of drug-resistant bacteria underscores the need to search for innovative and nature-based solutions. One of the approaches may be the use of plants that constitute a rich source of miscellaneous compounds with a wide range of biological properties. This review explores the antimicrobial activity of seven bioactives and their possible molecular mechanisms of action. Special attention was focused on the antibacterial properties of berberine, catechin, chelerythrine, cinnamaldehyde, ellagic acid, proanthocyanidin, and sanguinarine against Staphylococcus aureus, Enterococcus spp., Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, Serratia marcescens and Pseudomonas aeruginosa. The growing interest in novel therapeutic strategies based on new plant-derived formulations was confirmed by the growing number of articles. Natural products are one of the most promising and intensively examined agents to combat the consequences of the overuse and misuse of classical antibiotics.

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Section: Klebsiella Pneumoniaementioning

confidence: 99%

Bioactive Compounds from Plant Origin as Natural Antimicrobial Agents for the Treatment of Wound Infections

Pacyga,

Topola

et al. 2024

IJMS

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Biological characterization and in vitro fungicide screening of a new causal agent of walnut leaf spot in Guizhou Province, China

Ao,

Shi,

Yang

et al. 2024

Front. Microbiol.

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Walnut (Juglans regia L.) is a widely grown nut plant worldwide, including in Guizhou Province, located in southwest China. The high quality and special taste make Guizhou walnuts, particularly those produced in Hezhang County, a “Chinese National Geographical Indication Product” that substantially contributes to the local economy and grower’s income. In July 2022, a serious occurrence of leaf spot disease was observed in a walnut plantation area, Shuitang Town, Hezhang County, Guizhou Province, China (27°07′67″N, 104°64′61″E). The causal agent was identified as Didymella segeticola through morphological characterization and amplification and sequencing of the internal transcribed spacer (ITS) region, beta-tubulin (TUB) gene, and glyceraldehyde-3-phosphate dehydrogenase (G3PD) gene. Koch’s postulates, including re-isolation and identification, were performed to confirm its pathogenicity on healthy leaves. To our knowledge, this is the first report of D. segeticola causing leaf spot on walnuts worldwide. Further, to determine its biological characteristics, which could be utilized for future disease management, the effects of temperature, light, and carbon and nitrogen resources on mycelial growth, conidia production, and conidia germination and the effects of humidity on conidia germination were studied. The optimum temperature for mycelial growth of representative strain D. segeticola C27 was 20°C. Increasing the light period significantly decreased conidia production and conidia germination. Maltose and beef extract were the best carbon and nitrogen sources, respectively, for the pathogen. Conidia germination was enhanced at 90% humidity. In vitro screening of effective fungicides was conducted. Among the 20 screened fungicides, difenoconazole showed the best inhibition rate, with an EC50 (concentration for 50% of the maximal effect) of 0.0007 μg/mL. Tetramycin also showed sufficient inhibitory effects against D. segeticola, with an EC50 value of 0.0009 μg/mL. Our study provides new insights into the causal agent of walnut leaf spot in Guizhou, China, as well as the first pathogen characteristics and promising candidate fungicides for its management.

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Antimicrobial and Antifungal Action of Biogenic Silver Nanoparticles in Combination with Antibiotics and Fungicides Against Opportunistic Bacteria and Yeast

Rozhin,

Batasheva,

Iskuzhina

et al. 2024

IJMS

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The development of multidrug resistance by pathogenic bacteria and yeast is a significant medical problem that needs to be addressed. One possible answer could be the combined use of antibiotics and silver nanoparticles, which have different mechanisms of antimicrobial action. In the same way, these nanoparticles can be combined with antifungal agents. Biogenic silver nanoparticles synthesized using environmentally friendly biosynthesis technology using extracts of biologically active plants are an effective nanomaterial that needs to be comprehensively investigated for implementation into medical practice. In this study, the synergistic effects arising from their combined use with antibiotics and fungicides against various bacteria and yeasts were studied. The following methods were used: disco-diffusion analysis and construction of plankton culture growth curves. The synergistic effect of silver nanoparticles and antibiotics (fungicides) has been determined. Effective concentrations of substances were established, recommendations for the studied pathogenic species were presented, and the effect of destruction of the bacterial membrane was illustrated. The most significant synergistic effect was manifested in pathogenic candida and brewer’s yeast.

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Characterization and Isolation of the Major Biologically Active Metabolites Isolated from Ficus retusa and Their Synergistic Effect with Tetracycline against Certain Pathogenic-Resistant Bacteria

Cited by 3 publications

References 36 publications

Bioactive Compounds from Plant Origin as Natural Antimicrobial Agents for the Treatment of Wound Infections

Bioactive Compounds from Plant Origin as Natural Antimicrobial Agents for the Treatment of Wound Infections

Biological characterization and in vitro fungicide screening of a new causal agent of walnut leaf spot in Guizhou Province, China

Antimicrobial and Antifungal Action of Biogenic Silver Nanoparticles in Combination with Antibiotics and Fungicides Against Opportunistic Bacteria and Yeast

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