Characterization and Mapping of a Salt‐Sensitive Mutant in Rice (Oryza sativaL.)

Abstract

A salt-sensitive mutant designated rice salt sensitive 2 (rss2) was isolated from the M2 generation of the rice cultivar Nipponbare mutagenized with ethyl methanesulfonate (EMS). This mutant exhibited a greater decrease in salt tolerance with a significant increase in Na(+) content in its shoots. Genetic analysis indicated that the increase in Na(+) in rss2 was controlled by a single recessive gene. Further genome-wide analysis of the linkage map constructed from the F2 population of rss2/Zhaiyeqing 8 (ZYQ8) showed that two quantitative trait loci (QTLs) on chromosomes 1 and 6 were responsible for the Na(+) concentration in shoots, which explained 14.5% and 53.3%, respectively, of the phenotypic variance. The locus on chromosome 1, but not that on chromosome 6, was also detected in the F2 population of Nipponbare/ZYQ8, suggesting that the QTL on chromosome 6 was responsible for the salt sensitivity in rss2. By analyzing the recombination events in 220 mutant individuals of an enlarged mapping population of rss2/ZYQ8, the rss2 locus was precisely mapped to an interval of 605.3 kb between insertion/deletion (InDel) markers IM21962 and IM22567. This finding will facilitate the cloning of the rss2 locus and provide insight into the physiological mechanisms of salt sensitivity in rice.

Phytochrome B Negatively Affects Cold Tolerance by Regulating OsDREB1 Gene Expression through Phytochrome Interacting Factor-Like Protein OsPIL16 in Rice

“…The sterilized seeds were soaked in water at room temperature for 2 days and then germinated for 1 day at 28°C. Seedlings were grown hydroponically in Yoshida’s culture solution as described previously (Zhou et al, 2013). Plants were cultured in a growth chamber at 28°C/28°C (day/night) under a 15-h-light/9-h-dark photoperiod.…”

The Rice High-Affinity Potassium Transporter1;1 Is Involved in Salt Tolerance and Regulated by an MYB-Type Transcription Factor

“…The seeds were soaked in water at room temperature for 3 d and then germinated for 1 d at 28°C. Seedlings were grown hydroponically in Yoshida's culture solution as described previously (Zhou et al, 2013). Plants were cultured in a growth chamber at 28°C/25°C (day/night) under a 14-hlight/10-h-dark photoperiod (approximately 500 mmol m 22 s…”

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

“…The mutant lines exhibiting significantly higher grain yield or higher tolerance to nutrient deficiency or abiotic stresses or other traits can be used for rice breeding by conventional backcrossing along with molecular marker-assisted selection (Jiang and Ramachandran, 2010;Sikora et al, 2011). EMS induced rice mutants were used to investigate the complex mechanism involved in water and salt stress tolerance (Huang et al, 2009;Zhou et al, 2013). The morphological, physiological and proteomic characterization of some EMS induced mutants showed more tolerance to abiotic stresses for example salt and heat in contrast to their respective wild type lines (Ghaffari et al, 2014;Poli et al, 2013;Nakhoda et al, 2012;Mithra et al, 2016).…”