Characterization and Mapping of Expressed Sequence Tags Isolated From a Subtracted Cdna Library of Litopenaeus Vannamei Injected With White Spot Syndrome Virus

Alcivar–Warren, Acacia; Song, Linsheng; Meehan-Meola, Dawn; Xu, Zhenkang; Xiang, Jianhai; Warren, William

doi:10.2983/0730-8000(2007)26[1247:camoes]2.0.co;2

Cited by 8 publications

(4 citation statements)

References 61 publications

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“…For example, an EST from our asymptomatic-enriched library (GO271347) was highly similar (210/270 identical nucleotides, blastn e =1e-48) to a sequence identified in a SSH library constructed from shrimp injected with the white spot syndrome virus (CX535856, [25]). The function and phylogenetic history of these genes are not known.…”

Section: Resultsmentioning

confidence: 97%

Altered gene expression associated with epizootic shell disease in the American lobster, Homarus americanus

Tarrant

Stegeman

Verslycke

2010

Fish & Shellfish Immunology

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Epizootic shell disease is a poorly understood condition that has significantly affected the American lobster fishery in New England (northeastern US) since the 1990s. Here we present the results of a study to identify changes in gene expression in lobsters exhibiting symptoms of epizootic shell disease. Suppressive subtractive hybridization (SSH) was used to compare gene expression between cDNA pools from diseased (symptomatic) and apparently healthy (asymptomatic) lobsters. Subsequently, quantitative real-time polymerase chain reaction (qPCR) was used to measure expression of nine genes that were differentially-expressed in the SSH analysis, in seven tissues (muscle, gill, heart, hepatopancreas, brain, branchiostegite, gonad) dissected from individual symptomatic and asymptomatic lobsters. Expression of arginine kinase (involved in cellular energetics) was significantly decreased in muscle of symptomatic lobsters. Expression of hemocyanin (a respiratory hemolymph protein involved in oxygen transport) was highest in hepatopancreas and showed highly variable expression with a trend toward higher expression in asymptomatic individuals. 2-Macroglobulin (involved in the innate immune system) was most highly expressed in the ovary, particularly of symptomatic lobsters. The ESTs produced through this study add to the fledgling field of crustacean genomics and revealed three genes that could be further evaluated in lobsters of varying shell disease severity, molt stage, and reproductive condition, for possible implication in epizootic shell disease.

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Section: Resultsmentioning

confidence: 97%

Altered gene expression associated with epizootic shell disease in the American lobster, Homarus americanus

Tarrant

Stegeman

Verslycke

2010

Fish & Shellfish Immunology

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“…20 and with ESTs, mapping actin , myosin light chain and troponin I , over-expressed in WSSV infected L. vannamei and reported by Alcivar-Warren et al . 46 .…”

Section: Discussionmentioning

confidence: 99%

Temporal changes in transcriptome profile provide insights of White Spot Syndrome Virus infection in Litopenaeus vannamei

Peruzza

Shekhar

Kumar

et al. 2019

Sci Rep

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Shrimp aquaculture is severely affected by WSSV. Despite an increasing effort to understand host/virus interaction by characterizing changes in gene expression (GE) following WSSV infection, the majority of published studies have focussed on a single time-point, providing limited insight on the development of host-pathogen interaction over the infection cycle. Using RNA-seq, we contrasted GE in gills of Litopenaeus vannamei at 1.5, 18 and 56 hours-post-infection (hpi), between WSSV-challenged and control shrimps. Time course analysis revealed 5097 differentially expressed genes: 63 DEGs were viral genes and their expression in WSSV group either peaked at 18 hpi (and decreased at 56 hpi) or increased linearly up to 56 hpi, suggesting a different role played by these genes during the course of infection. The remaining DEGs showed that WSSV altered the expression of metabolic, immune, apoptotic and cytoskeletal genes and was able to inhibit NF-κB and JAK/STAT pathways. Interestingly, GE changes were not consistent through the course of infection but were dynamic with time, suggesting the complexity of host-pathogen interaction. These data offer novel insights into the cellular functions that are affected during the course of infection and ultimately provide a valuable resource towards our understanding of the host-pathogen dynamics and its variation with time.

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“…L. vannamei are the most commonly farmed species of shrimp in the world (Ciobanu et al 2010), making them interesting to geneticists who want to improve their disease resistance (e.g. Alcivar-Warren et al 2007b;Dhar et al 2007;Gross et al 2001). Researchers have focused on discovering and mapping microsatellite markers in Pacific white shrimp (Alcivar-Warren et al 2007a, b;Meehan et al 2003), instead of the easier to use SNPs commonly found across the genome.…”

Section: Snp Discovery Methodsmentioning

confidence: 99%

“…The The Pacific white shrimp has 44 pairs of chromosomes (2n = 88) and a genome of ~2.5 × 10 9 base pairs (Chow et al 1990). Unfortunately, very limited genome sequence information is available for penaeid shrimp species (Tassanakajon et al 2006;Alcivar-Warren et al 2007b;. Several preliminary genetic maps for Pacific white shrimp have already been published, mostly composed of amplified fragment length polymorphisms and a small number of microsatellite markers (Meehan et al 2003;Pérez et al 2005;Alcivar-Warren et al 2006, 2007aDhar et al 2007;Garcia & Alcivar-Warren, 2007;Zhang et al 2007), and genetic maps for other penaeid shrimp species, e.g.…”

Section: Introductionmentioning

confidence: 99%

The prediction of single nucleotide polymorphisms and their utilization in mapping traits and determining population structure in production animals

Gorbach¹

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Characterization and Mapping of Expressed Sequence Tags Isolated From a Subtracted Cdna Library of Litopenaeus Vannamei Injected With White Spot Syndrome Virus

Cited by 8 publications

References 61 publications

Altered gene expression associated with epizootic shell disease in the American lobster, Homarus americanus

Altered gene expression associated with epizootic shell disease in the American lobster, Homarus americanus

Temporal changes in transcriptome profile provide insights of White Spot Syndrome Virus infection in Litopenaeus vannamei

The prediction of single nucleotide polymorphisms and their utilization in mapping traits and determining population structure in production animals

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