Characterization and molecular mapping of stripe rust resistance in wheat – <i>Psathyrostachys huashanica</i> introgression line H9015-17-1-9-6

Sun, Cai; Liu, Yike; Chao, Kai Xiang; Fang, Zheng Wu; Wang, Shu Ping; Tang, Qing; Fang, Dong; Yin, Jun; Zhu, Yong Xing

doi:10.1080/07060661.2018.1523230

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…At present, four stripe rust resistance genes from wheat– P . huashanica translocation lines have been identified by our group (Cao et al., 2008; Li et al., 2012; Ma et al., 2013, 2016, 2019; Sun et al., 2019). These genes possess high level of resistance to the tested Pst races.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, several excellent resistance genes to most Chinese Pst races were found from P. huashanica . The resultant genes were temporarily named YrH9015 (Sun et al., 2019), YrH921 (Ma et al., 2019), YrHu (Ma et al., 2016), YrHA (Ma et al., 2013), YrH9020 (Li et al., 2012), YrHy (Yao et al., 2010) and YrHua (Cao et al ., 2008). In this study, the wheat translocation line H921‐11‐1 possesses resistance to all Chinese Pst races from 1990 to the present.…”

Section: Introductionmentioning

confidence: 99%

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Molecular mapping of stripe rust resistance gene YrH921 in a wheat introgression line H921‐11‐1

Jiang

Sun

et al. 2020

Journal of Phytopathology

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Several novel stripe rust pathogen races emerging in the wheat‐planting regions of China in recent years were virulent to a majority of the designated wheat seedling resistance genes. Therefore, it is of great significance to continuously select more new and valuable materials for enriching resistant sources diversity, pyramiding different excellent resistance genes and achieving durable resistance. In this study, a resistance gene, temporarily designated as YrH921, was identified from wheat–Psathyrostachys huashanica introgression line H921‐11‐1. Two hybrid populations, 160 F2 plants and corresponding derived F2:3 families, of the two parents about Mingxian169 as female and H921‐11‐1 as male, were used to evaluate stripe rust resistance in seedling stage and as a mapping population. At last, a genetic map which comprises of four simple sequence repeat (SSR) markers and six expressed sequence tag (EST) markers was constructed. YrH921 was located on the long arm of chromosome 5A. Two closely linked EST‐STS markers (BF483937 and BF484913) were screened, and the genetic distance linked to YrH921 was 3.0 and 4.3 cM, respectively. There was great value in research and production if the two closest markers were effectively used for marker‐assisted selection of YrH921 in breeding.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular mapping of stripe rust resistance gene YrH921 in a wheat introgression line H921‐11‐1

Jiang

Sun

et al. 2020

Journal of Phytopathology

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“…Since 1990, many of its excellent traits have been transferred to common wheat by wide hybridization [7,8] and created some derived lines with P. huashanica chromosome fragments: Such as addition lines 20JH1155 [9], substitution lines H139 [10], translocation lines TR77 [11], etc., these derived offspring outperform their parents in biological and abiotic stressors, as well as agronomic qualities. So far, some important genes controlling excellent traits in derived lines have also been explored, such as the identi cation of stripe rust resistance genes YrHu [12] and YrH9015 [13]; The resistance to take-all gene of wheat -P. huashanica introgression line H148 was located in the 68.8-70.1 Mb interval of chromosome 2As [14]; 7Ns of wheat -P. huashanica disomic addition line 2-1-6 showed resistance to leaf rust [15]. The development of these materials demonstrates that P. huashanica is an important germplasm resource for wheat genetic improvement.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic identification and molecular marker development of a novel Wheat - Psathyrostachys huashanica 2NsS (2D) fragment disomic substitution line with resistance to stripe rust

Wang,

Ge,

Deng

et al. 2024

Preprint

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Background Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) is a rare and unique species of grass that is native to China. It is a significant genetic resource for wheat breeding because of the multiple beneficial features that can be used in wheat improvement programs. Results The study isolated a novel wheat - P. huashanica derived line 18TR61 from the progeny of common wheat 7182 and P. huashanica. Cytological studies revealed that 18TR61 has chromosome karyotype of 2n = 42 = 21II; Genomic in situ hybridization (GISH) and non-denaturing fluorescence in situ hybridization (ND-FISH) experiments confirmed that two 2D chromosomes in line 18TR61 were replaced by two incomplete Ns chromosomes. Meanwhile, it has been observed that 18TR61 exhibits structural variations in chromosomes 2B, 7B, and 5A when compared to its parents. Molecular markers and GenoBaits®WheatplusPh microarray analysis indicated that the alien chromosomes originated from P. huashanica 2Ns. Based on karyotype observations, it was incomplete in length, and contains all of the short arm, centromere, and fragment of the long arm. Assessment of agronomic traits showed that 18TR61 outperformed its parents in terms of stripe rust resistance in the adult plant stage, as well as spike length and grain size. Conclusions As a result, 18TR61 was identified as a wheat - P. huashanica 2NsS (2D) fragment disomic substitution. Furthermore, the established 15 specific markers were beneficial for the quick identification of heterologous material containing the 2Ns chromosome in the wheat background. This line offered a possible bridge for wheat genetic improvement on disease resistance to stripe rust and yield.

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“…These progeny lines outperformed their wheat parents in terms of abiotic and biotic stress resistance and agronomic traits. To date, there has been some progress in the mapping of important genes in the P. huashanica genome, including those conferring resistance to stripe rust and take-all ( Ma et al., 2016 ; Bai et al., 2021 ; Sun et al, 2018 ). Therefore, P. huashanica is generally considered to be a potentially useful germplasm for the genetic improvement of wheat.…”

Section: Introductionmentioning

confidence: 99%

RNA-seq analysis revealed considerable genetic diversity and enabled the development of specific KASP markers for Psathyrostachys huashanica

Zhang

Zeng

et al. 2023

Front. Plant Sci.

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Psathyrostachys huashanica, which grows exclusively in Huashan, China, is an important wild relative of common wheat that has many desirable traits relevant for wheat breeding. However, the poorly characterized interspecific phylogeny and genomic variations and the relative lack of species-specific molecular markers have limited the utility of P. huashanica as a genetic resource for enhancing wheat germplasm. In this study, we sequenced the P. huashanica transcriptome, resulting in 50,337,570 clean reads that were assembled into 65,617 unigenes, of which 38,428 (58.56%) matched at least one sequence in public databases. The phylogenetic analysis of P. huashanica, Triticeae species, and Poaceae species was conducted using 68 putative orthologous gene clusters. The data revealed the distant evolutionary relationship between P. huashanica and common wheat as well as the substantial diversity between the P. huashanica genome and the wheat D genome. By comparing the transcriptomes of P. huashanica and Chinese Spring, 750,759 candidate SNPs between P. huashanica Ns genes and their common wheat orthologs were identified. Among the 90 SNPs in the exon regions with different functional annotations, 58 (64.4%) were validated as Ns genome-specific SNPs in the common wheat background by KASP genotyping assays. Marker validation analyses indicated that six specific markers can discriminate between P. huashanica and the other wheat-related species. In addition, five markers are unique to P. huashanica, P. juncea, and Leymus species, which carry the Ns genome. The Ns genome-specific markers in a wheat background were also validated regarding their specificity and stability for detecting P. huashanica chromosomes in four wheat–P. huashanica addition lines. Four and eight SNP markers were detected in wheat–P. huashanica 2Ns and 7Ns addition lines, respectively, and one marker was specific to both wheat–P. huashanica 3Ns, 4Ns, and 7Ns addition lines. These markers developed using transcriptome data may be used to elucidate the genetic relationships among Psathyrostachys, Leymus, and other closely-related species. They may also facilitate precise introgressions and the high-throughput monitoring of P. huashanica exogenous chromosomes or segments in future crop breeding programs.

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Characterization and molecular mapping of stripe rust resistance in wheat – Psathyrostachys huashanica introgression line H9015-17-1-9-6

Cited by 5 publications

References 29 publications

Molecular mapping of stripe rust resistance gene YrH921 in a wheat introgression line H921‐11‐1

Molecular mapping of stripe rust resistance gene YrH921 in a wheat introgression line H921‐11‐1

Cytogenetic identification and molecular marker development of a novel Wheat - Psathyrostachys huashanica 2NsS (2D) fragment disomic substitution line with resistance to stripe rust

RNA-seq analysis revealed considerable genetic diversity and enabled the development of specific KASP markers for Psathyrostachys huashanica

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