Characterization and ontogenesis of nitric oxide synthase activity in pig enterocytes

M'Rabet-Touil, Hamida; Blachier, François; Morel, Marie-Thérèse; Darcy‐Vrillon, Béatrice; Duée, Pierre‐Henri

doi:10.1016/0014-5793(93)80345-u

Cited by 30 publications

(2 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is also worth noting that the gut in the newborn pigs, although more mature that in newborn rodents, is, however, less mature than in infants (128). Another advantage of the pig model is that it is possible to recover a large number of cells (for instance, absorptive intestinal cells from both the small and large intestine after dietary intervention), even in young animals, in order to measure the impact of such intervention on cell metabolism and physiology (129, 130). In the pig model, preterm delivery at 90% gestation is comparable to preterm infant born at approximately 75% gestation (30 weeks) (131), making the pig neonate an interesting model for pediatric studies.…”

Section: The Pig Model For Research On Obesitymentioning

confidence: 99%

Animal Models for the Study of the Relationships between Diet and Obesity: A Focus on Dietary Protein and Estrogen Deficiency

et al. 2017

Self Cite

View full text Add to dashboard Cite

Obesity is an increasing major public health concern asking for dietary strategies to limit weight gain and associated comorbidities. In this review, we present animal models, particularly rats and mice, which have been extensively used by scientists to understand the consequences of diet quality on weight gain and health. Notably, modulation of dietary protein quantity and/or quality has been shown to exert huge effects on body composition homeostasis through the modulation of food intake, energy expenditure, and metabolic pathways. Interestingly, the perinatal window appears to represent a critical period during which the protein intake of the dam can impact the offspring’s weight gain and feeding behavior. Animal models are also widely used to understand the processes and mechanisms that contribute to obesity at different physiological and pathophysiological stages. An interesting example of such aspect is the situation of decreased estrogen level occurring at menopause, which is linked to weight gain and decreased energy expenditure. To study metabolic disorders associated with such situation, estrogen withdrawal in ovariectomized animal models to mimic menopause are frequently used. According to many studies, clear species-specific differences exist between rats and mice that need to be taken into account when results are extrapolated to humans.

show abstract

Section: The Pig Model For Research On Obesitymentioning

confidence: 99%

Animal Models for the Study of the Relationships between Diet and Obesity: A Focus on Dietary Protein and Estrogen Deficiency

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…L-arginine can also be converted to nitric oxide and L-citrulline through the catalytic activities of nitric oxide synthase (NOS, see Figure 1) which are present in colon absorptive epithelial cells (20).…”

Section: Metabolism Of L-arginine In the Polyamine And Nitric Oxide Pmentioning

confidence: 99%

Channelling of arginine in NO and polyamine pathways in colonocytes and consequences

Blachier¹

2011

Front Biosci

View full text Add to dashboard Cite

Colon epithelium is renewed within few days through asymmetric mitosis of pluripotent cells followed by differentiation and exfoliation. Absorptive colonocytes do not transport amino acids from lumen to bloodstream but import amino acids from plasma. Among amino acids, colonocytes can use L-arginine as a precursor for nitric oxide (NO) synthesis and also for polyamine synthesis through the stepwise conversion of L-arginine into L-ornithine and urea, conversion of L-ornithine into putrescine and then synthesis of spermidine and spermine. Colonic epithelial cells can transport polyamines produced exogenously by the microbiota. Polyamines are strictly necessary for undifferentiated colonic epithelial cell proliferation but NO exerts anti-proliferative effect on these cells raising the view that the channelling of arginine in the nitric oxide and polyamine pathways is involved in the control of cellular proliferation. Furthermore, NO has been shown to be a potent inhibitor of ornithine decarboxylase activity in colonic epithelial cells. Unbalance of the chanelling of arginine in the NO and polyamine pathways in colonic cancerous epithelial cells as a determinant promoting their proliferation is discussed.

show abstract

Polyamines Inhibit Lipopolysaccharide-Induced Nitric Oxide Synthase Activity in Rat Liver Cytosol

1997

View full text Add to dashboard Cite

Characterization and ontogenesis of nitric oxide synthase activity in pig enterocytes

Cited by 30 publications

References 35 publications

Animal Models for the Study of the Relationships between Diet and Obesity: A Focus on Dietary Protein and Estrogen Deficiency

Animal Models for the Study of the Relationships between Diet and Obesity: A Focus on Dietary Protein and Estrogen Deficiency

Channelling of arginine in NO and polyamine pathways in colonocytes and consequences

Polyamines Inhibit Lipopolysaccharide-Induced Nitric Oxide Synthase Activity in Rat Liver Cytosol

Contact Info

Product

Resources

About