Characterization and optimization of bacterial cellulose produced by Acetobacter spp.

Anusuya, R.S.; Anandham, Rangasamy; Kumutha, K.; Gayathry, G.; Mageshwaran, M.; Uthandi, S.

doi:10.22438/jeb/41/4/mrn-994

Cited by 7 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, the addition of agar at a concentration of 0.6% in the stirred-tank reactor generated 11.6 g L − 1 of BC by Acetobacter xylinum BPR2001 [49]. In the present study, addition of 1% PEG 6000 as polymer additive in HS medium enhanced the BC yield as reported earlier [28,50].…”

Section: Discussionsupporting

confidence: 82%

“…The most efficient producer of BC is a Gram-negative, acetic acid bacterium, Acetobacter xylinum (reclassified as Gluconacetobacter xylinus) [26,27]. In this study, A. senegalensis MA1, isolated previously [28] from sugarcane juice, was used for optimizing BC production. Our earlier results demonstrated that A. senegalensis MA1 produced the maximum amount of cellulose mat, registering 7.2 g L − 1 on a dry weight basis in HS broth in 2 weeks of culturing [28].…”

Section: Discussionmentioning

confidence: 99%

“…In this study, A. senegalensis MA1, isolated previously [28] from sugarcane juice, was used for optimizing BC production. Our earlier results demonstrated that A. senegalensis MA1 produced the maximum amount of cellulose mat, registering 7.2 g L − 1 on a dry weight basis in HS broth in 2 weeks of culturing [28]. Although the standard recommended medium for the BC production is HS medium [29], researchers have continuously attempted to optimize the media and process parameters for improving BC yields [30].…”

Section: Discussionmentioning

confidence: 99%

“…The cellulose producing bacteria, A. senegalensis MA1 [28] obtained from the Department of Agricultural Microbiology, Agricultural College and Research Institute, Madurai -625,104, was previously isolated from sugarcane juice. The isolate was maintained in standard Hestrin-Schramm (HS) medium [68] composed of glucose-20 g L − 1 , peptone-5 g L − 1 , yeast extract-5 g L − 1 , disodium hydrogen phosphate (Na 2 HPO 4 )-2.7 g L − 1 , citric acid-1.15 g L − 1 and agar-20 g L − 1 .…”

Section: Microorganisms and Mediamentioning

confidence: 99%

See 3 more Smart Citations

Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1

2020

View full text Add to dashboard Cite

Background: Cellulose, the most versatile biomolecule on earth, is available in large quantities from plants. However, cellulose in plants is accompanied by other polymers like hemicellulose, lignin, and pectin. On the other hand, pure cellulose can be produced by some microorganisms, with the most active producer being Acetobacter xylinum. A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose. Besides, the production is also influenced by other culture conditions. Previously, we isolated and identified A. senengalensis MA1, and characterized the bacterial cellulose (BC) produced. Results: The maximum cellulose production by A. senengalensis MA1 was pre-optimized for different parameters like carbon, nitrogen, precursor, polymer additive, pH, temperature, inoculum concentration, and incubation time. Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM). Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5°C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation. The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L − 1 of glycerol, 7.50 g L − 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5°C along with 7.76 g L − 1 of PEG 6000. This gave a BC yield of wet weight as 469.83 g L − 1. Conclusion: The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium. Further, the cellulose produced can be used in food and pharmaceuticals, for producing high-quality paper, wound dressing material, and nanocomposite films for food packaging.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Microorganisms and Mediamentioning

confidence: 99%

See 2 more Smart Citations

Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1

2020

View full text Add to dashboard Cite

show abstract

“…Next is cellulose biosynthesis by using different types of microorganisms; (i) bacteria (gram-negative: Alcaligenes [ 149 ], Salmonella , Enterobacter , Pseudomonas [ 150 ], Gluconacetobacter xylinus [ 151 ], Agrobacterium [ 152 ], Komagataeibacter Medellinensis [ 153 ], Aerobacter , Achromobacter insuavis [ 154 ], Rhizobium leguminosarum [ 155 ], Acetobacter spp. [ 156 ], Acetobacter xylinum [ 157 ], Zoogloea [ 97 ], and gram-positive: Sarcina ventriculi [ 158 ], Leifsonia sp [ 159 ], Rhizosphere bacterium , Bacillus subtilis [ 70 , 160 ]); (ii) fungi ( Aspergillus ornatus [ 161 ], Penicillium sp. [ 162 , 163 ], Aspergillus terreus MS105 [ 164 ], Aspergillus terreus M1 [ 165 ], Aspergillus niger , Rhizopus sp.…”

Section: Principal Pathways Of Cellulose Productionmentioning

confidence: 99%

Use of Industrial Wastes as Sustainable Nutrient Sources for Bacterial Cellulose (BC) Production: Mechanism, Advances, and Future Perspectives

et al. 2021

View full text Add to dashboard Cite

A novel nanomaterial, bacterial cellulose (BC), has become noteworthy recently due to its better physicochemical properties and biodegradability, which are desirable for various applications. Since cost is a significant limitation in the production of cellulose, current efforts are focused on the use of industrial waste as a cost-effective substrate for the synthesis of BC or microbial cellulose. The utilization of industrial wastes and byproduct streams as fermentation media could improve the cost-competitiveness of BC production. This paper examines the feasibility of using typical wastes generated by industry sectors as sources of nutrients (carbon and nitrogen) for the commercial-scale production of BC. Numerous preliminary findings in the literature data have revealed the potential to yield a high concentration of BC from various industrial wastes. These findings indicated the need to optimize culture conditions, aiming for improved large-scale production of BC from waste streams.

show abstract

Bacterial Cellulose Applied in Wound Dressing Materials: Production and Functional Modification – A Review

Hou,

Xia,

Pan

et al. 2023

Macromolecular Bioscience

View full text Add to dashboard Cite

In recent years, the development of new type wound dressings has gradually attracted more attention. Bacterial cellulose (BC) is a natural polymer material with various unique properties, such as ultrafine three‐dimensional nano‐network structure, high water retention capacity and biocompatibility. These properties allow BC to be used independently or in combination with different components (such as biopolymers and nanoparticles) to achieve diverse effects. This means that BC has great potential as a wound dressing. However, systematic summaries for the production and commercial application of BC‐based wound dressings are still lacked. Therefore, this review provides a detailed introduction to the production fermentation process of BC, including various production strains and their biosynthetic mechanisms. Subsequently, with regard to the functional deficiencies of bacterial cellulose as a wound dressing, recent research progress in this area are enumerated. Finally, prospects are discussed for the low‐cost production and high‐value‐added product development of BC‐based wound dressings.This article is protected by copyright. All rights reserved

show abstract

Characterization and optimization of bacterial cellulose produced by Acetobacter spp.

Cited by 7 publications

References 0 publications

Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1

Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1

Use of Industrial Wastes as Sustainable Nutrient Sources for Bacterial Cellulose (BC) Production: Mechanism, Advances, and Future Perspectives

Bacterial Cellulose Applied in Wound Dressing Materials: Production and Functional Modification – A Review

Contact Info

Product

Resources

About