Characterization and partial purification of the Croatian national standard <i>Dermatophagoides pteronyssinus</i> allergen extract

Tresščcec, A.; Kolevska, T.; Cvorišsšcec, B.; Krnić, Branka; Stipić-Marković, Asja; Tuđman, Zdenko; Dekaris, Dragan

doi:10.1111/j.1398-9995.1993.tb00744.x

Cited by 6 publications

(7 citation statements)

References 24 publications

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“…For measurement of ¿)/>/-spccific IgG4 the RIA test developed in Institute of Immunology, Zagreb, was used as described previously (19). The examined sera were incubated with paper disks covalently coupled with Dpt allergen (Institute of Immunology, Zagreb) for 3 h at room temperature.…”

Section: Measurement O F Total Serum Ige Level Serum-specific Ige Anmentioning

confidence: 99%

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Expression of Lymphocytes FcεRII/CD23 in Allergic Children Undergoing Hyposensitization

Gagro

Rabatić²,

Trešćec³

et al. 1993

Int Arch Allergy Immunol

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Owing to the proposed role of FcεRII/CD23 in allergic diseases, we analyzed the expression of this receptor on peripheral blood lymphocytes (pan-B, pan-T and CD4+ or CD8+ T cells) and its autoproteolytic product sCD23 in serum. This was done in 10 asthmatic children allergic to Dermatophagoides pteronyssinus(Dpt) before and 6 weeks after hyposensitization. FACS analysis of double, direct immunofluorescence staining of the whole blood revealed an elevated percentage of FcεRII/CD23+ lymphocytes in allergic children (10.29 ± 5.0), a significantly higher percentage than in nonallergic children (5.7 ± 2.4, p < 0.05). The majority of FcεRII/CD23+ were on B cells. A significant positive correlation between the percentages of CD23+ lymphocytes and serum IgE levels was found (Spearman rank = 0.63, p < 0.05). The percentage of CD20+CD23+ lymphocytes significantly decreased after 6 weeks of hyposensitization (6.2 ± 3.6, p < 0.05), while the percentage of CD20+ lymphocytes remained unchanged. Similarly, hyposensitization was followed by a reduction of total serum IgE levels, but Dpt-specific IgG4 and IgE remained unchanged.

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Section: Measurement O F Total Serum Ige Level Serum-specific Ige Anmentioning

confidence: 99%

“…The lyophilized and standardized Dpt-extract (Institute of Im munology, Zagreb) [19] was used for both immunotherapy and tests. The relative potency of the allergen extract, which were determined and compared with the WHO International Standard for Dpt by RAST inhibition assay, was 6.5 x HP IU.…”

Section: Allergen Extractmentioning

confidence: 99%

Expression of Lymphocytes FcεRII/CD23 in Allergic Children Undergoing Hyposensitization

Gagro

Rabatić²,

Trešćec³

et al. 1993

Int Arch Allergy Immunol

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“…Belgium) were added and incubated overnight at room temperature. Radioactivity was determined by a '/-counter (ANSR Gamma Counter, Abbott Laboratories, North Chicago, III.. USA) [20]. All tests were duplicated and results expressed as the counts/min ratio.…”

Section: Total Serum Ige Dcr-p-speciftc Ige Anti !Gg4mentioning

confidence: 99%

“…The lyophilized Der p allergen extract (Institute o f Immunology, Zagreb, Croatia), which has been accepted as the Croatian national standard, was used for RIT and inhalation challenge [20]. Briefly, dried house-dust mite Dermatophagoidespteronyssinus was extracted in the ratio 1:100 w/v in PBS.…”

Section: Allergen Extractmentioning

confidence: 99%

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Decrease in CD23+ B Lymphocytes and Clinical Outcome in Asthmatic Patients Receiving Specific Rush Immunotherapy

Kljaić-Turkalj¹,

Čvoriščec²,

Tudorić³

et al. 1996

Int Arch Allergy Immunol

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Rush immunotherapy (RIT) has been documented as useful in the treatment of patients with allergic bronchial asthma. To investigate the mechanisms of its action, we studied changes in the serum levels of total IgE, allergen-specific IgE and IgG4, and expression of CD23 on peripheral blood B cells in patients receiving RIT. Twenty patients with perennial bronchial asthma were evaluated before the beginning of RIT, as well as 6 weeks and 6 months later. Compared to pre-treatment values, the level of Der-p-specific IgG4 and IgE significantly increased after 6 weeks and 6 months of RIT, while the total serum IgE remained unchanged. Furthermore, after 6 months of RIT, the percentage of CD23+ B cells and its CD23 receptor density significantly decreased. Since the symptom score improved and the need for medication decreased, we evaluated RIT as a useful procedure. After 6 months, 30% of patients did not have an asthma attack, with no medication in the last month, while 10% of them were asthma free for the last 3 months. No significant correlation between the clinical improvement, and in vitro changes was found. Furthermore, the observed in vitro changes were not significantly different in patients who responded with clinical improvement, compared to those with unchanged intensity of asthma. In conclusion, during specific RIT we found a significant increase in Der-p-specific IgE and IgG4 antibodies, as well as a moderate decrease in CD23+ B cells and its CD23 receptor density. These findings suggest a change in the lymphokine profile of patients receiving specific immunotherapy, and that the inhibition of IL-4-induced B cell stimulation may be hypothesized as the most important mechanism.

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Allergen‐induced CD23 on CD4⁺ T lymphocytes and CD21 on B lymphocytes in Patients with allergic asthma: Evidence and regulation

Gagro

Rabatić

1994

Eur J Immunol

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Interaction of CD4+ T cells and B cells is necessary for IgE production. It has been recently demonstrated that cell surface antigen CD21 is a ligand for CD23 (Fc epsilon RII) and that the pairing of these molecules may participate in the control of IgE production. In this study we investigated the effect of the Dermatophagoides pteronyssinus (Dpt) allergen and recombinant interleukin(rIL)-4 on the expression of CD21 and CD23 on T and B cells of asthmatic patients allergic to Dpt and of healthy controls. Peripheral blood mononuclear cells (PBMC) were incubated alone or with Dpt allergen (100 biological units/ml) and/or rIL-4 (100 U/ml) for up to 7 days. The flow-cytometric analysis of double-fluorescence staining revealed that Dpt allergen and/or rIL-4 induced CD23 on CD4+ T lymphocytes only in allergic patients. The allergen-induced CD23 on T cells is de novo synthesized antigen since no induction of CD23 on T cells was observed in cultures with 0.4 microgram/ml actinomycin D. Moreover, 100 U/ml of interferon-gamma inhibited the induction of CD23 on CD4+ T cells. T cells obtained from healthy donors did not express CD23 or CD21 antigen upon incubation with allergen and/or rIL-4. Although rIL-4 also induced CD23 in controls, the expression was only observed on CD20+ cells. The allergen alone induced a significant elevation of the mean fluorescence intensity of both CD21 and CD23 only in allergic individuals. When the cell proliferation was analyzed, a slightly increased stimulation index upon cultivation of PBMC was obtained from non-allergic donors as well, but less than in allergic patients. The co-expression of major histocompatibility complex class II molecules and CD23 on CD4+ T lymphocytes in allergic patients, as assessed by the three-color immunofluorescence analysis, indicates that these cells were activated. We conclude that CD4+ T lymphocytes possess a unique capability to express CD23 upon exposure to allergen. Moreover, the allergen-mediated induction of CD23 on T cells observed only in allergic patients may be the reason for the increase of IgE production. This would not occur in non-allergic individuals as there is no CD23 expression on T cells.

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Characterization and partial purification of the Croatian national standard Dermatophagoides pteronyssinus allergen extract

Cited by 6 publications

References 24 publications

Expression of Lymphocytes FcεRII/CD23 in Allergic Children Undergoing Hyposensitization

Expression of Lymphocytes FcεRII/CD23 in Allergic Children Undergoing Hyposensitization

Decrease in CD23+ B Lymphocytes and Clinical Outcome in Asthmatic Patients Receiving Specific Rush Immunotherapy

Allergen‐induced CD23 on CD4⁺ T lymphocytes and CD21 on B lymphocytes in Patients with allergic asthma: Evidence and regulation

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Characterization and partial purification of the Croatian national standard Dermatophagoides pteronyssinus allergen extract

Cited by 6 publications

References 24 publications

Expression of Lymphocytes FcεRII/CD23 in Allergic Children Undergoing Hyposensitization

Expression of Lymphocytes FcεRII/CD23 in Allergic Children Undergoing Hyposensitization

Decrease in CD23+ B Lymphocytes and Clinical Outcome in Asthmatic Patients Receiving Specific Rush Immunotherapy

Allergen‐induced CD23 on CD4+ T lymphocytes and CD21 on B lymphocytes in Patients with allergic asthma: Evidence and regulation

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Allergen‐induced CD23 on CD4⁺ T lymphocytes and CD21 on B lymphocytes in Patients with allergic asthma: Evidence and regulation