Characterization and pathogenicity of acute hepatopancreatic necrosis disease natural mutants, pirAB  (‐) V. parahaemolyticus, and pirAB  (+) V. campbellii strains

Han, Jee Eun; Tang, Kathy F.J.; Aranguren, Luis Fernando; Piamsomboon, Patharapol

doi:10.1016/j.aquaculture.2016.12.022

Cited by 53 publications

(43 citation statements)

References 23 publications

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“…Interestingly, missing of the fragment was seen on a plasmid sharing homology with pVA1 from a non-AHPND-causing strain M2-36, suggesting that a natural deletion or insertion of pirAB vp might have occurred (Lee et al, 2015). Other variants with partial deletion were also found (Han et al, 2017). The plasmid pVA1 also carries a cluster of genes related to conjugative transfer (Lee et al, 2015); hence, this plasmid may potentially be able to transfer not only among V. parahaemolyticus strains but also to different bacterial species.…”

Section: Introductionmentioning

confidence: 88%

“…The plasmid pVA1 also carries a cluster of genes related to conjugative transfer (Lee et al, 2015); hence, this plasmid may potentially be able to transfer not only among V. parahaemolyticus strains but also to different bacterial species. Indeed, the existence of pirAB vp has been reported in harveyi -like Vibrio (one isolate from Vietnam) (Kondo et al, 2015), V. owensii (one isolate from China) (Liu et al, 2015; Xiao et al, 2017) and V. campbellii (one isolate from China and four from Latin America) (Dong et al, 2017a; Han et al, 2017). We have previously reported the first AHPND-causing V. campbellii (VC AHPND ) strain, 20130629003S01, isolated from Guangxi, China, which contains the pirAB vp and showed pathogenicity in shrimp (Dong et al, 2017a).…”

Section: Introductionmentioning

confidence: 99%

“…Outbreaks of AHPND have been reported in recent years in Asia (such as China, Vietnam, Malaysia, Philippines, Thailand) and Latin America (such as Mexico) (Gomez-Gil et al, 2014; Gomez-Jimenez et al, 2014; Kondo et al, 2014, 2015; Nunan et al, 2014; Yang et al, 2014; de la Pena et al, 2015; Lee et al, 2015; Soto-Rodriguez et al, 2015; Chonsin et al, 2016; Restrepo et al, 2016; Han et al, 2017). The disease has caused dramatic drops of shrimp production in affected areas, leading to an estimated loss of over $1 billion per year to the global shrimp farming industry (FAO, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Earlier researches revealed that it is caused by certain virulent strains of Vibrio parahaemolyticus , namely AHPND-causing V. parahaemolyticus (VP AHPND ) (Zhang et al, 2012; Tran et al, 2013; Lee et al, 2015). Recently, it was reported that the bacterial etiology of AHPND also includes harveyi -like Vibrio (Kondo et al, 2015), V. owensii (Liu et al, 2015; Xiao et al, 2017) and V. campbellii (Dong et al, 2017a; Han et al, 2017). …”

Section: Introductionmentioning

confidence: 99%

“…The Pir vp toxin is encoded by the pirA vp and pirB vp genes that are located within a fragment delimited by two identical inversely-oriented insertion sequences (IS) IS Val1 . It is believed the two IS Val1 with the region in between form a composite transposon called Tn 6264 (Han et al, 2017) or pirAB -Tn 903 (Xiao et al, 2017). Interestingly, missing of the fragment was seen on a plasmid sharing homology with pVA1 from a non-AHPND-causing strain M2-36, suggesting that a natural deletion or insertion of pirAB vp might have occurred (Lee et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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pirABvp-Bearing Vibrio parahaemolyticus and Vibrio campbellii Pathogens Isolated from the Same AHPND-Affected Pond Possess Highly Similar Pathogenic Plasmids

Dong

Wang

et al. 2017

Front. Microbiol.

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Acute hepatopancreatic necrosis disease (AHPND) is a severe shrimp disease originally shown to be caused by virulent strains of Vibrio parahaemolyticus (VPAHPND). Rare cases of AHPND caused by Vibrio species other than V. parahaemolyticus were reported. We compared an AHPND-causing V. campbellii (VCAHPND) and a VPAHPND isolate from the same AHPND-affected pond. Both strains are positive for the virulence genes pirABvp. Immersion challenge test with Litopenaeus vannamei indicated the two strains possessed similar pathogenicity. Complete genome comparison showed that the pirABvp-bearing plasmids in the two strains were highly homologous, and they both shared high homologies with plasmid pVA1, the reported pirABvp-bearing plasmid. Conjugation and DNA-uptake genes were found on the pVA1-type plasmids and the host chromosomes, respectively, which may facilitate the dissemination of pirABvp. Novel variations likely driven by ISVal1 in the genetic contexts of the pirABvp genes were found in the two strains. Moreover, the VCAHPND isolate additionally contains multiple antibiotic resistance genes, which may bring difficulties to control its future outbreak. The dissemination of the pirABvp in non-parahaemolyticus Vibrio also rises the concern of missing detection in industrial settings since the isolation method currently used mainly targeting V. parahaemolyticus. This study provides timely information for better understanding of the causes of AHPND and molecular epidemiology of pirABvp and also appeals for precautions to encounter the dissemination of the hazardous genes.

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Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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pirABvp-Bearing Vibrio parahaemolyticus and Vibrio campbellii Pathogens Isolated from the Same AHPND-Affected Pond Possess Highly Similar Pathogenic Plasmids

Dong

Wang

et al. 2017

Front. Microbiol.

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Use of Direct‐Fed Microbes to Enhance Shrimp Resistance to a Vibrio Parahaemolyticus Strain Causing Early Morality Syndrome (EMS)

Kuhn,

Smith,

Williams

et al. 2024

Aquaculture Fish & Fisheries

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Early mortality syndrome (EMS) or acute hepatopancreatic necrosis syndrome (AHPND) is an epizootic bacterial infection of shrimp that has had significant negative impact on the global shrimp aquaculture industry. This disease is largely attributed to pathogenic Vibrio parahaemolyticus EMS strains (VP‐EMS). Application of probiotics was examined for control of pathogenic VP‐EMS infections in Pacific white shrimp (Litopenaeus vannamei) under laboratory‐scale conditions. Two probiotic products, a single strain (O14VRQ) of Bacillus subtilis and a blend (Plus10) of five strains of Bacillus from four different species (Bacillus amyloliquifaciens, Bacillus megaterium, Bacillus brevis, and two distinct strains of Bacillus subtilis), were evaluated at various concentrations as feed additives (applied as a top coat on commercial feed) or dosed directly into the culture water. Two trials were conducted in which shrimp were fed either a control feed (no probiotics) or probiotic‐coated feed for 7 days prior to be being challenged with VP‐EMS. Shrimp were observed for clinical signs of disease and mortalities during the disease challenge study. These experiments demonstrated that while both Bacillus probiotic products were shown to significantly (p < 0.05) improve shrimp survival, the O14VRQ strain provided the most consistent protection across the trials in top‐coated feed. Overall, better efficacy was observed with probiotic‐coated feeds. Findings were directly and positively correlated with concentration for top‐coated and even more so for direct in‐tank applications. Collectively, these findings contribute to our understanding about how probiotic strains could be applied to enhance shrimp health in aquaculture.

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A Broad-Host-Range Phage Cocktail Selectively and Effectively Eliminates Vibrio Species from Shrimp Aquaculture Environment

et al. 2022

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Characterization and pathogenicity of acute hepatopancreatic necrosis disease natural mutants, pirAB (‐) V. parahaemolyticus, and pirAB (+) V. campbellii strains

Cited by 53 publications

References 23 publications

pirABvp-Bearing Vibrio parahaemolyticus and Vibrio campbellii Pathogens Isolated from the Same AHPND-Affected Pond Possess Highly Similar Pathogenic Plasmids

pirABvp-Bearing Vibrio parahaemolyticus and Vibrio campbellii Pathogens Isolated from the Same AHPND-Affected Pond Possess Highly Similar Pathogenic Plasmids

Use of Direct‐Fed Microbes to Enhance Shrimp Resistance to a Vibrio Parahaemolyticus Strain Causing Early Morality Syndrome (EMS)

A Broad-Host-Range Phage Cocktail Selectively and Effectively Eliminates Vibrio Species from Shrimp Aquaculture Environment

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