Characterization and pathogenicity of sweet potato (Ipomoea batatas) black rot caused by Ceratocystis fimbriata in Korea

Paul, Narayan Chandra; Nam, Sang-Sik; Kachroo, Aardra; Kim, Yun-Hee; Yang, Jung‐Wook

doi:10.1007/s10658-018-1522-8

Cited by 27 publications

(21 citation statements)

References 17 publications

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“…To assess the biocontrol efficiency of P. dispersa in the leaves and tuberous roots of the sweet potato cultivar “Yulmi”, which is susceptible to C. fimbrata infection 23 , samples were pre-treated with RO-21 or water (as a control) and then inoculated at the same site with droplets containing C. fimbriata spores. The disease incidence on the leaves and tuberous roots was then determined.…”

Section: Resultsmentioning

confidence: 99%

“…Sweet potato [ Ipomoea batatas (L.)] is one of the most important cultivated crops in the world, with an annual production area of 8.0 million hectares and a total global production of 106,569 million tons 20 . Sweet potato is also a valuable medicinal plant due to its anti-cancer, anti-diabetic, anti-oxidant, and anti-inflammatory activities 21–23 . However, sweet potato plants experience many post-harvest diseases during transportation and storage.…”

Section: Introductionmentioning

confidence: 99%

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Potential of Pantoea dispersa as an effective biocontrol agent for black rot in sweet potato

Jiang

Jeong

Lee

et al. 2019

Sci Rep

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Biocontrol offers a promising alternative to synthetic fungicides for the control of a variety of pre- and post-harvest diseases of crops. Black rot, which is caused by the pathogenic fungus Ceratocytis fimbriata, is the most destructive post-harvest disease of sweet potato, but little is currently known about potential biocontrol agents for this fungus. Here, we isolated several microorganisms from the tuberous roots and shoots of field-grown sweet potato plants, and analyzed their ribosomal RNA gene sequences. The microorganisms belonging to the genus Pantoea made up a major portion of the microbes residing within the sweet potato plants, and fluorescence microscopy showed these microbes colonized the intercellular spaces of the vascular tissue in the sweet potato stems. Four P. dispersa strains strongly inhibited C. fimbriata mycelium growth and spore germination, and altered the morphology of the fungal hyphae. The detection of dead C. fimbriata cells using Evans blue staining suggested that these P. dispersa strains have fungicidal rather than fungistatic activity. Furthermore, P. dispersa strains significantly inhibited C. fimbriata growth on the leaves and tuberous roots of a susceptible sweet potato cultivar (“Yulmi”). These findings suggest that P. dispersa strains could inhibit black rot in sweet potato plants, highlighting their potential as biocontrol agents.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential of Pantoea dispersa as an effective biocontrol agent for black rot in sweet potato

Jiang

Jeong

Lee

et al. 2019

Sci Rep

Self Cite

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“…The dried leaves were inoculated with agar plugs and spore suspension methods. The fungus was grown on PDA for 5 days at 25 • C and 5 mm agar plugs were taken and placed onto the adaxial and abaxial sides of leaves with wounds; non-mycelium plugs served as a control [21,22]. Spores were collected, counted using a hemocytometer, and adjusted to 1 × 10 5 spores/mL.…”

Section: Pathogenicitymentioning

confidence: 99%

Morphology, Molecular Phylogeny, and Pathogenicity of Neofusicoccum parvum, Associated with Leaf Spot Disease of a New Host, the Japanese Bay Tree (Machilus thunbergii)

Choi

Paul

Lee

et al. 2021

Forests

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During a survey of diseased plants on Wando Island, Korea from May to June 2020, a severe leaf spot disease was observed in the upper leaves of Japanese bay tree (Machilus thunbergii). Early symptoms were light blackish spots on the leaf surface and enlargement of older spots. Dry leaf spots surrounded with deep black margins were common throughout the plants. Symptomatic leaf samples were collected, and the causal pathogen was isolated on potato dextrose agar (PDA). Three fungal isolates (CMML20-1, CMML20-3, and CMML20-4) were cultured on PDA for morphological characterization at 25 °C in the darkness. Fungal colonies were circular, fast-growing, olivaceous to dark grey, and with abundant aerial mycelium. Sporulation was induced in 14 h-10 h light-dark conditions, and the conidia were single-celled, thin-walled with a smooth surface, ellipsoid with round apices, and measuring 17.5–20.5 (avg. 17.5) μm × 7.5–10.0 (7.9) μm. The morphological characteristics resembled those typical for Neofusicoccum parvum. Molecular identification was confirmed by partially sequencing the internal transcribed spacer (ITS) region and the translation elongation factor 1-α (EF1-α) genes. Pathogenicity tests were conducted on detached leaves and whole plants of M. thunbergii. High disease prevalence was observed, and Koch postulates were fulfilled. This is the first worldwide report of N. parvum causing leaf spots on Machilus thunbergii.

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“…During C. fimbriata infection, sweet potato is known to biosynthesize sesquiterpenoids, which are toxic for humans 4 . Relevant spreads of black rot disease have taken place in different locations during recent years 5,6 . The losses in crop yield and postharvest preservation have been estimated from 60 to 80% loss, which corresponds to more than 150 million dollars 7 …”

Section: Introductionmentioning

confidence: 99%

Mode of action and efficacy of quinolinic acid for the control of Ceratocystis fimbriata on sweet potato

Chen

Zhou

Laborda

et al. 2021

Pest Management Science

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BACKGROUND Ceratocystis fimbriata is a hazardous fungal pathogen able to cause black rot disease on sweet potato. The management of C. fimbriata strongly relies on the use of toxic fungicides, and there is a lack of efficient alternative strategies. RESULTS The antifungal properties of quinolinic acid (QA) were studied for the first time, indicating that QA shows selective antifungal activity against C. fimbriata. QA inhibited completely the mycelial growth of C. fimbriata at less than 0.8 mg mL–1 concentration (pH 4), and was able to produce alterations in the fungal cell wall, and to impede spore agglutination and mycelium formation. QA significantly reduced the concentration of ergosterol, and was able to associate to iron (II), suggesting that QA may be a lanosterol 14‐α demethylase inhibitor. In preventive applications, QA reduced the disease incidence of C. fimbriata on sweet potato by 75%, achieving higher control efficacy in comparison with commercial fungicides prochloraz and carbendazim. CONCLUSIONS The first selective antifungal agent against C. fimbriata was discovered in this work, and showed suitable antifungal properties for the management of black rot disease. © 2021 Society of Chemical Industry.

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Characterization and pathogenicity of sweet potato (Ipomoea batatas) black rot caused by Ceratocystis fimbriata in Korea

Cited by 27 publications

References 17 publications

Potential of Pantoea dispersa as an effective biocontrol agent for black rot in sweet potato

Potential of Pantoea dispersa as an effective biocontrol agent for black rot in sweet potato

Morphology, Molecular Phylogeny, and Pathogenicity of Neofusicoccum parvum, Associated with Leaf Spot Disease of a New Host, the Japanese Bay Tree (Machilus thunbergii)

Mode of action and efficacy of quinolinic acid for the control of Ceratocystis fimbriata on sweet potato

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