Characterization and Phylogenetic Analysis of Brazilian Chicken Anaemia Virus

Simionatto, Simone; Lima-Rosa, Carlos André da Veiga; Binneck, Eliseu; Ravazzolo, Ana Paula; Canal, Cláudio Wageck

doi:10.1007/s11262-005-0033-9

Cited by 19 publications

(10 citation statements)

References 30 publications

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“…There were four unique amino acid mutations (L/M/F113P, K/S/V341R, V357A, and A371V) in VP1 of CAV variant, which were not previously reported (Figure 1). The VP1 residue 394 is a major genetic determinant of virulence, with glutamine (Q) and histidine (H) representing high and low pathogenicity, respectively [19, 20]. In the CAV variant, residue 394 was glutamine (Q) and was, therefore, highly pathogenic.…”

Section: Resultsmentioning

confidence: 99%

Identification of a Chicken Anemia Virus Variant-RelatedGyrovirusin Stray Cats in China, 2012

Zhang

Liu

et al. 2014

BioMed Research International

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The chicken anemia virus (CAV), is a known member of the genus Gyrovirus and was first isolated from chickens in Japan in 1979. Some reports have also demonstrated that CAV can be identified in human stool specimens. In this study, a variant of CAV was detected using PCR with CAV-based primers in fecal samples of stray cats. The genome of CAV variant was sequenced and the results suggest that it could be a recombinant viral strain from parental CAV strains JQ690762 and AF311900. Recombination is an important evolutionary mechanism that contributes to genetic diversification. These findings indicate that CAV variant might have originated from CAV-infected chickens. The epidemiology and pathogenesis of this novel virus remains to be elucidated. This study underscores the importance of CAV surveillance and it presents the first evidence suggesting the possibility of CAV homologous recombination in cat.

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Section: Resultsmentioning

confidence: 99%

Identification of a Chicken Anemia Virus Variant-RelatedGyrovirusin Stray Cats in China, 2012

Zhang

Liu

et al. 2014

BioMed Research International

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“…In the hypervariable region of VP1 from position 139 to 151, the GD-D-12 isolate showed two mutations, W146C and P147S, while the GD-I-12 isolate only contained a single mutation in the hypervariable region (P147S). Residue 394 in VP1 was a major genetic determinant of virulence, with glutamine (Q) and histidine (H) representative of highly pathogenic and less pathogenic viruses, respectively2526. All of the southern Chinese CAV isolates contained a glutamine at position 394, suggesting that they all represented highly pathogenic viruses.…”

Section: Resultsmentioning

confidence: 99%

“…In our study, we also compared the 13 CAV sequences to the 54 other complete CAV sequences that are listed in GenBank which contain most of the VP1, VP2 and VP3 gene sequences. Genetic typing according to partial CAV sequences has been used as a means of tracing the spread of CAV1426. In China, previous research only focused on either the VP1 gene or the approximately full-length CAV genomes.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic and molecular characterization of chicken anemia virus in southern China from 2011 to 2012

Zhang

Liu

et al. 2013

Sci Rep

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Chicken anemia virus (CAV) is an important pathogen that causes severe immunosuppression in young chickens. We have characterized 13 CAVs isolated from different commercial farms in southern China between 2011 and 2012. We discovered 92 variable residues compared to 37 other CAV complete genome sequences from other parts of the world listed in GenBank; these residues have not been previously observed. All of the Chinese CAV genomes that were characterized in this study had a glutamine at position 394, a hallmark of highly pathogenic CAVs. We also discovered that intra-group genetic recombination plays a role in generating genetic diversity in natural populations of CAV. The GD-J-12 isolate was a possible recombinant between GD-C-12 and GD-M-12 in the genomic region that encompassed both the coding and non-coding regions.

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“…These imported products were significantly cheaper than the locally sourced chickens and may have been preferentially purchased and consumed by a large proportion of the South African population. Simionatto et al [30] reported that CAV was detected in 90% of field samples collected from commercial breeders, broilers, and free-range chickens from different regions of Brazil. Whether these chickens are infected with CAV either naturally or through vaccination programs is not known.…”

Section: Discussionmentioning

confidence: 99%

Novel Gyroviruses, including Chicken Anaemia Virus, in Clinical and Chicken Samples from South Africa

Smuts

2014

Advances in Virology

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Introduction. Chicken anaemia virus, CAV, was until recently the only member of the Gyrovirus genus. 6 novel gyroviruses, AGV2, HGyV1, and GyV3-6, have since been discovered in human and chicken samples. Methods. PCR amplification of the VP2 gene was used to detect AGV2/HGyV1, GyV3, and CAV in a range of clinical samples including stool, respiratory, CSF, and HIV-positive plasma. Screening of fresh local chicken meat was also performed. Results. AGV2/HGyV1 or GyV3 was detected in stools from healthy children (17/49, 34.7%) and patients with diarrhoea (22/149, 14.8%). 1.2% (3/246) nasopharyngeal respiratory samples were positive. No AGV2/HGyV1 or GyV3 was detected in nasal swabs from wheezing patients, in CSF from patients with meningitis, and in HIVpositive plasma. CAV was found in 51% (25/49) of stools from healthy children and 16% (24/149) in diarrhoea samples. Screening of 28 chicken samples showed a higher prevalence of gyrovirus (20/28, 71%) compared to CAV (1/28, 3.6%). Phylogenetic analysis of the CAV VP1 gene showed South African sequences clustering with Brazilian isolates from genotypes D2 and A2. Conclusion. Novel gyroviruses, including CAV, are present in the South African population with diarrhoea and respiratory illness as well as in healthy children. Their presence suggests an origin from chicken meat consumption.

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Characterization and Phylogenetic Analysis of Brazilian Chicken Anaemia Virus

Cited by 19 publications

References 30 publications

Identification of a Chicken Anemia Virus Variant-RelatedGyrovirusin Stray Cats in China, 2012

Identification of a Chicken Anemia Virus Variant-RelatedGyrovirusin Stray Cats in China, 2012

Phylogenetic and molecular characterization of chicken anemia virus in southern China from 2011 to 2012

Novel Gyroviruses, including Chicken Anaemia Virus, in Clinical and Chicken Samples from South Africa

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