Characterization and protective effect of Polygonatum sibiricum polysaccharide against cyclophosphamide-induced immunosuppression in Balb/c mice

Liu, Na; Dong, Zhen; Zhu, Xiaosong; Xu, Hongya; Zhao, Zhongxi

doi:10.1016/j.ijbiomac.2017.09.051

Cited by 134 publications

(74 citation statements)

References 41 publications

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“…At the same time, LJP also has a weaker absorption peak at near 2900 cm -1 , which is caused by vibration of C-H bonds in sugar compounds. These two absorption peaks are characteristic absorption peaks of sugar compounds [ 4 ]. The absorption peak at 1635 cm -1 is a characteristic absorption peak of the crystalline water of sugar compounds.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, polysaccharides have medicinal functions including immunomodulatory function, anti-bacterial, anti-virus, and anti-tumor [ 2 , 3 ]. For example, polygonatum sibiricum polysaccharide can enhance T cell and B cell proliferation responses and restore the levels of IL-2, TNF-α, IL-8 and IL-10 in the serum of the Cyclophosphamide (CTX)-treated mice [ 4 ]. Lycium ruthenicum Murr .…”

Section: Introductionmentioning

confidence: 99%

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Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide

et al. 2018

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Lonicera japonica is a typical Chinese herbal medicine. We previously reported a method to isolate polysaccharides from Lonicera japonica (LJP). In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method. We then investigated the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models. The results showed that LJP had the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides. In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice. LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice. Finally, the results on measuring the T-lymphocytes subsets of spleen also confirmed LJP-induced immunomodulatory activity in immunosuppressed mice from another perspective. Therefore, LJP could be used as a potential immunomodulatory agent.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide

et al. 2018

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“…In the three spectra, the absorption peaks at approximately 1610-1655 cm −1 corresponded to carbonyl groups (C=O) and were caused by the bending mode of bound water, while the absorption peak at approximately 1400 cm −1 was the C-O stretching vibration [26,29,34,35]. A strong absorption peak at 1615 cm −1 presented carboxyl groups (COO−) in GUPS-III, indicating the existence of uronic acids [36,37]. A strong band between 950 and 1160 cm −1 in the three spectra of GUPS were attributed to the stretching vibrations of the pyranose ring [38].…”

Section: The Analysis Of Gups By Ft-ir Spectroscopymentioning

confidence: 99%

Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

et al. 2020

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Glycyrrhiza uralensis is a Chinese herbal medicine with various bioactivities. Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively. The monosaccharide compositions of GUPS-II and GUPS-III were similar, while that of GUPS-I was distinctively different. The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages. Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I. An in vivo experiment showed that only GUPS-II significantly enhanced the maturation of DCs. These results indicate that GUPS-II has the potential to be used in combination with cancer immunotherapy to enhance the therapeutic effect.

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“…The major extract from Polygonatum sibiricum is Polygonatum sibiricum polysaccharides (PSP) mainly consisting of mannose, galactose, glucose, and xylose, which is also rich in various bioactive components such as alkaloids, steroidal saponins, flavonoids, and lignin [3,4]. In recent years, PSP has been reported to have many pharmacological applications and biological activities, such as antioxidant, antiaging, antiosteoporosis, antidiabetes, anticancer, and antifatigue, as well as immune enhancement, neuroprotection [5,6].…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Polygonatum sibiricum Polysaccharides on Apoptosis, Inflammation, and Oxidative Stress in Nucleus Pulposus Cells of Rats with the Degeneration of the Intervertebral Disc

Zhai

et al. 2019

International Journal of Polymer Science

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Objective. Polygonatum sibiricum polysaccharide (PSP) has antioxidant activity, immune enhancement, and other biological properties. However, the effect of PSP on intervertebral disc degeneration has not been reported. In this study, we mainly investigated the effect of PSP on the apoptosis, inflammation, and oxidative stress of nucleus pulposus cells (NPCs) during the process of intervertebral disc degeneration. Methods. A rat NPC model induced by H2O2 was constructed. The CCK8 method was used to measure the effects of PSP on the apoptosis of rat NPCs induced by H2O2. The effects on the activity of SOD and content of MDA were also determined. The rat model of intervertebral disc degeneration was treated with PSP for 1 month, and the mRNA expression levels of IL-1β, COX2, iNOS, Col2α1, Col10α1, and MMP3 were measured by qPCR in the tissue of intervertebral disc. NPCs from the degenerated intervertebral discs were separated, and the cell viability was measured by the CCK8 method. The contents of SOD and MDA in NPCs were determined as well. Results. PSP significantly reduced the apoptosis of NPCs induced by H2O2, significantly increased the SOD content, and decreased the content of MDA in H2O2-induced NPCs. The expression level of IL-1β, COX2, and iNOS in the rat model with intervertebral disc degeneration was significantly downregulated after 1 month of PSP treatment. PSP treatment increased the expression of Col2α1 type and significantly decreased the expression of Col10α1 type collagen and MMP3 in rats with disc degeneration. PSP treatment significantly reduced NPC apoptosis and increased its SOD content and reduced MDA content, which is consistent with the results from cell-level experiments. Conclusion. PSP can effectively reduce the apoptosis, inflammation, and oxidative stress of H2O2-induced NPCs in rats with intervertebral disc degeneration and mitigate the progression of intervertebral disc degeneration, which has the potential to be developed as new drugs for the treatment of intervertebral disc degeneration.

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Characterization and protective effect of Polygonatum sibiricum polysaccharide against cyclophosphamide-induced immunosuppression in Balb/c mice

Cited by 134 publications

References 41 publications

Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide

Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide

Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

Protective Effect of Polygonatum sibiricum Polysaccharides on Apoptosis, Inflammation, and Oxidative Stress in Nucleus Pulposus Cells of Rats with the Degeneration of the Intervertebral Disc

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