Characterization and quantification of 10‐hydroxycamptothecine in Camptotheca acuminate and its medicinal preparation by liquid chromatography–ion trap mass spectrometry

Abstract

A rapid and sensitive method for the identification and quantification of 10-hydroxycamptothecine (HCPT) in Camptotheca acuminata Decne is described. The HCPT standard solution was directly infused into the ion trap mass spectrometers (IT/MS) for collecting the MS(n) spectra. The electrospray ionization (ESI) mass spectral fragmentation pathway of HCPT was proposed and the ESI-MS(n) fragmentation behavior of HCPT was deduced in detail. The major fragment ions of HCPT were confirmed by MS(n) in both negative ion and positive ion mode. The possible main cleavage pathway of fragment ions was studied. Quantification of HCPT was assigned in negative-ion mode at a product ion at m/z 363 → 319 by LC-MS. The LC-MS method was validated for linearity, sensitivity, accuracy and precision, and then used to determine the content of the HCPT. Lastly, the LC-MS method was successfully applied to determine HCPT in real samples of Camptotheca acuminate Decne and its medicinal preparation in the first time.

Comprehensive characterization and quantification of phillyrin in the fruits ofForsythia suspensaand its medicinal preparations by liquid chromatography-ion trap mass spectrometry

“…It had identified the active compounds of Chinese herbs in our previous reports [22,23]. Moreover, these reports show that IT mass spectrometers can also be very useful for quantitative analysis.…”

Simultaneous determination of camptothecin and 10‐hydroxycamptothecine in the Camptotheca acuminate, its medicinal preparation and in rat plasma by liquid chromatography with fluorescence detection

“…The results obtained by LC-FLD were compared with the results reported for LC (Chen et al, 2013b;Zhao et al, 2010;Zu et al, 2004) as estimated by the Student t-test at 95% confidence level, which indicated no significant difference between the LC-FLD method and the reference reported by LC and LC-MS methods. Analysis of real samples was performed on C. acuminata and HCPT injection extracted with methanol under the above conditions.…”

“…In order to accurate evaluate and control the quality of C. acuminate and the preparation process of related drugs by CPT and HCPT with low concentrations, it is necessary to establish a reliable and accurate analytical method for the determination of CPT and HCPT (Chen et al, 2013a). In recent years, liquid chromatography (LC) with various detection systems has become the most common approach for the determination of CPT and HCPT in medicinal plants and their medicinal preparations (Chen et al, 2013b;Huang et al, 2005;Lan et al, 2003;Tsaia et al, 2000;Zu et al, 2004). In recent years, liquid chromatography (LC) with various detection systems has become the most common approach for the determination of CPT and HCPT in medicinal plants and their medicinal preparations (Chen et al, 2013b;Huang et al, 2005;Lan et al, 2003;Tsaia et al, 2000;Zu et al, 2004).…”

“…In recent years, liquid chromatography (LC) with various detection systems has become the most common approach for the determination of CPT and HCPT in medicinal plants and their medicinal preparations (Chen et al, 2013b;Huang et al, 2005;Lan et al, 2003;Tsaia et al, 2000;Zu et al, 2004). In particular, mass spectrometric (MS) detection has become increasingly widespread for the determination of analytes in medicinal plants (Chen et al, 2013b;Zhao et al, 2010), but the high cost of the instrumentation and the different polarities and ionization capabilities of analytes still limit the use of MS for many laboratories worldwide. In particular, mass spectrometric (MS) detection has become increasingly widespread for the determination of analytes in medicinal plants (Chen et al, 2013b;Zhao et al, 2010), but the high cost of the instrumentation and the different polarities and ionization capabilities of analytes still limit the use of MS for many laboratories worldwide.…”

Study the voltammetric behavior of 10-Hydroxycamptothecin and its sensitive determination at electrochemically reduced graphene oxide modified glassy carbon electrode

“…To date, high-performance liquid chromatography (HPLC) (Ma et al, 2002), LC-fluorescence or ion trap mass spectrometry (Zheng et al, 2012;Chen et al, 2013b), chemiluminescence (Sun et al, 2011), and LC-ESI-MS/MS (Zhao et al, 2010), were extensively used to detect HCPT. Although widely employed and possessing excellent selectivity and high sensitivity, these methods are high-cost and consume much longer time and labor.…”