Characterization and Quantification of Phenolic Compounds from Leaf of Agarista salicifolia

Abstract

Agarista salicifolia is the wild tree and has been used as important medicinal plants in South Ethiopia since a long time ago. The aim of this work was to carry out a chemical analysis focusing on secondary metabolites, particularly phenolic compounds, which have several roles in the plant physiological processes and have demonstrated significant capacity in the prevention of human health diseases. Phenolic acids, flavonols, flavan-3-ols, and dihydrochalcones were characterized and quantified using ultra high-performance liquid chromatography coupled with diode array and electrospray ionization mass spectrometric detection (LC-MS), FTIR, and UV-Visible spectroscopy techniques. After extraction with ethanol, compounds were characterized and quantified based on retention time, molecular ions, and the comparison with reference compounds. Six flavan-3-ols, four phenolic acids, five flavonols, and two dihydrochalcones were identified and quantified in the extract. Chlorogenic (279.23 ± 28.75 µg/g of dried leaf) acid, Quercetin-3-O-rhaminoside (97.24 ± 4.07 µg/g of dried leaf), and epicatechin (304. 57 ± 20.88 µg/g of dried leaf), were the most abundant phenolic acid, flavonol, and flavanols, respectively. This is the first study on the phenolic composition of A. salicifolia, highlighting the importance of these natural products as a source of medicinal applications in pharmaceutical industries by herbal drug manufacturers.

Performance of dye-sensitized solar cells extracted dye from wood apple leaves

“…These observable bands in the spectra agree with the earlier reported FTIR results on the carotenoids (neoxanthin), flavonoids, phenolic, chlorophyll, and its derivatives [40][41][42][43][44]. The broadest band centered at 3336 cm −1 is ascribed to O-H groups or H-bond, indicating the presence of the phenolic compounds in the extract [42][43][44]. In the spectra beyond the fingerprint region, the response of the spectra at 2975, 2930, and 2889 cm −1 are due to antisymmetric and symmetric aliphatic saturated C-H stretching vibrations in methyl (-CH 3 ) and methylene (-CH 2 ) groups [40,[42][43][44].…”

“…All the distinct and sharp absorption bands are pronounced with their wavenumbers in the observed spectra, and corresponding functional groups are presented in table 1. These observable bands in the spectra agree with the earlier reported FTIR results on the carotenoids (neoxanthin), flavonoids, phenolic, chlorophyll, and its derivatives [40][41][42][43][44]. The broadest band centered at 3336 cm −1 is ascribed to O-H groups or H-bond, indicating the presence of the phenolic compounds in the extract [42][43][44].…”

Synthesis, characterization, antidiabetic and antioxidative evaluation of a novel Zn(II)-gallic acid complex with multi-facet activity

“…The IR spectroscopy interpretation was adopted from previous studies. [35][36][37] The phenolic -O-H stretching vibration peaks (broad) involved in intramolecular hydrogen bonding were observed in the FTIR absorption spectrum of gallic acid and complex in the range of 3200-3400 cm −1 (Figure 2) due to the presence of phenolic -OH group in gallic acid and its complex (Figure 1). This is further confirmed by the weak to medium phenolic -OH bend observed in gallic acid (1378 and 1306 cm −1 ) and its complex (1374, 1336 and 1307 cm −1 ) ( Figure 2).…”

Inhibitory effects of Manihot esculenta extracts on Food‐Borne pathogens and their antioxidant properties: Supercritical fluid extraction, statistical analysis, and molecular docking study

“…The peaks at 3,405.2 cm −1 correspond to the OH group, which confirmed the presence of the carboxyl group (Rai, Mohanty, & Bhargava, 2016). At 2,926.51 and 2,853.91 cm −1 , the presence of CH stretching in the methyl (CH 3 ) and methylene (CH 2 ) group is indicated (Hasana & Desalegn, 2017). The peak at 1,714.19 cm −1 is related to the CO stretch of the carbonyl group in the fatty acid (Tarhan, Ismail, & Kara, 2017).…”

“…The absorption of compounds present in the extract at a specific wavelength was analyzed using Lamda 35 UV–Vis spectrometer (Perkin Elmer, Boston, MA). The extract was dissolved in ethanol to 50 mg/ml by diluting 250 mg of extract into 5 ml ethanol and the absorption spectrum was recorded at 200 to 800 nm (Hasana & Desalegn, 2017).…”