Characterization and Quantification of Polyphenols and Triterpenoids in Thinned Young Fruits of Ten Pear Varieties by UPLC-Q TRAP-MS/MS

Sun, Li-Hua; Tao, Shutian; Zhang, Shaoling

doi:10.3390/molecules24010159

Cited by 67 publications

(46 citation statements)

References 36 publications

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“…The most abundant triterpene compound in all examined fruits was ursolic acid. This was in agreement with the results obtained by Sun et al [ 26 ]. In their study on triterpenoids in thinned young fruits of ten pear varieties, among 16 identifies triterpenoids, ursolic acid was predominant.…”

Section: Resultssupporting

confidence: 94%

“…Plants with high levels of pentacyclic triterpenes are often used in phytotherapy due to their valuable therapeutic properties. Numerous studies have shown a wide range of pro-health effects of triterpenes, such as antiviral, antitumor, anticancer, cytoprotective, and anti-inflammatory activities [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

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Bioactive Compounds and Health-Promoting Properties of Pear (Pyrus communis L.) Fruits

et al. 2020

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Consuming food that is rich in antioxidants reduces the risk of developing chronic diseases and oxidative stress. Fruits and vegetables are an excellent source of substances with antioxidant and pro-health properties. Such raw materials, characterized by a high content of polyphenolic compounds and antioxidant capacity, include pear fruits. In this study, the concentrations of bioactive compounds, as well as the antioxidant, anti-inflammatory, and antiproliferative activity in fruits of five selected pear cultivars were determined and compared. LC–MS and UPLC–PDA methods were used to determine the polyphenolic, carotenoid, chlorophyll, and triterpenoid profiles and content, and the antioxidant activity was analyzed using DPPH and ferric-reducing ability of plasma (FRAP) tests. The anti-inflammatory activity was determined against COX-1 and COX-2 enzymes. The cytotoxic activity of the test compounds was assessed against six tumor cell lines. The results showed that the major group of phenolic compounds in all cultivars was phenolic acids. In the group of chromoplastic pigments, chlorophyllide a and 9-cis-β-carotene were the major compounds, while in the triterpene group, ursolic acid was dominant. The antioxidant potential correlated with the content of polyphenols and carotenoids, and was the strongest for the ‘Radana’ cultivar. The highest antiproliferative activity in all varieties was established for bladder cancer.

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Section: Resultssupporting

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Bioactive Compounds and Health-Promoting Properties of Pear (Pyrus communis L.) Fruits

et al. 2020

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“…Although pears (Pyrus spp) are dietary sources of bioactive components such as polyphenols and triterpenic acids (39), the chemical composition of these fruits significantly differ from Bergamot and Wild Cardoon. Indeed, chlorogenic acid, quinic acid and arbutin are the primary polyphenols and ursolic acid is the predominant triterpenoid in thinned pears, whereas chlorogenic acid and most of the flavan-3-ols, are the main antioxidants in young pears (39).…”

Section: Discussionmentioning

confidence: 99%

Randomized Clinical Trial: Bergamot Citrus and Wild Cardoon Reduce Liver Steatosis and Body Weight in Non-diabetic Individuals Aged Over 50 Years

et al. 2020

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Background: Non-alcoholic fatty liver disease is the most common cause of liver-related morbidity and mortality in the world. However, no effective pharmacological treatment for this condition has been found. Purpose: This study evaluated the effect of a nutraceutical containing bioactive components from Bergamot citrus and wild cardoon as a treatment for individuals with fatty liver disease. The primary outcome measure was the change in liver fat content. Methods: A total of 102 patients with liver steatosis were enrolled in a double-blind placebo controlled clinical trial. The intervention group received a nutraceutical containing a Bergamot polyphenol fraction and Cynara Cardunculus extract, 300 mg/day for 12 weeks. The control group received a placebo daily. Liver fat content, by transient elastography, serum transaminases, lipids and glucose were measured at the baseline and the end of the study. Results: We found a greater liver fat content reduction in the participants taking the nutraceutical rather than placebo (−48.2 ± 39 vs. −26.9 ± 43 dB/m, p = 0.02); The percentage CAP score reduction was statistically significant in those with android obesity, overweight/obesity as well as in women. However, after adjustment for weight change, the percentage CAP score reduction was statistically significant only in those over 50 years (44 vs. 78% in placebo and nutraceutical, respectively, p = 0.007). Conclusions: This specific nutraceutical containing bioactive components from Bergamot and wild cardoon reduced the liver fat content during 12 weeks in individuals with liver steatosis over 50 years. If confirmed, this nutraceutical could become the cornerstone treatment of patients affected by liver steatosis. Clinical Trial Registration: www.isrctn.com, identifier ISRCTN12833814.

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“…Various polyphenols in pears, including arbutin, chlorogenic acid, and triterpenoids, including ursolic acid, have been reported [ 21 ]. Our results showed arbutin was not toxic in the given concentrations (≤100uM; Figure 3 ) and did not alleviate BPA toxicity ( Figure 5 ).…”

Section: Resultsmentioning

confidence: 99%

Micropillar/Microwell Chip Assessment for Detoxification of Bisphenol A with Korean Pear (Pyrus pyrifolia)

Lee

Koh

et al. 2020

Micromachines

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A micropillar/microwell chip platform with 3D cultured liver cells has been used for HTP screening of hepatotoxicity of bisphenol A (BPA), an endocrine-disrupting chemical. We previously found the hepatotoxicity of BPA is alleviated by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase 2 (ALDH2). In this study, we have tested potential BPA detoxification with Korean pear (Pyrus pyrifolia) extract, stimulators of ADH and ALDH, as well as arbutin, a reference compound in the pears, on the micropillar/microwell chip platform with human liver cells. Surprisingly, the toxicity of BPA was reduced in the presence of Korean pear extract, indicated by significantly increased IC50 values. The IC50 value of BPA with Korean pear extract tested against HepG2 cells was shifted from 151 to 451 μM, whereas those tested against Hep3B cells was shifted from 110 to 204 μM. Among the tested various concentrations, 1.25, 2.5, and 5 mg/mL of the extract significantly reduced BPA toxicity (Ps < 0.05). However, there was no such detoxification effects with arbutin. This result was supported by changes in protein levels of ADH in the liver cells.

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Characterization and Quantification of Polyphenols and Triterpenoids in Thinned Young Fruits of Ten Pear Varieties by UPLC-Q TRAP-MS/MS

Cited by 67 publications

References 36 publications

Bioactive Compounds and Health-Promoting Properties of Pear (Pyrus communis L.) Fruits

Bioactive Compounds and Health-Promoting Properties of Pear (Pyrus communis L.) Fruits

Randomized Clinical Trial: Bergamot Citrus and Wild Cardoon Reduce Liver Steatosis and Body Weight in Non-diabetic Individuals Aged Over 50 Years

Micropillar/Microwell Chip Assessment for Detoxification of Bisphenol A with Korean Pear (Pyrus pyrifolia)

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