Characterization and Quantitation of the Five Major Plasma Protein Fractions in Seven-Day-Old Piglets

Gal, Yoav

doi:10.1159/000240728

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…For example, fibrinogen concentrations vary from about 5 M in preterm human infants, 26 10 M in normal adult humans, 27 15 M in geriatric humans 28 and adult swine, 23 to 44 M for porcine infants (piglets)! 29 Thus, it can be anticipated that practical use of thrombin-binding anticoagulants may require some level of customization for use in human and veterinarian blood applications.…”

Section: Implications For Thrombin-binding Anticoagulants In Blood Comentioning

confidence: 99%

Contact activation of the plasma coagulation cascade. III. Biophysical aspects of thrombin-binding anticoagulants

Vogler

Nadeau

Graper

1998

J. Biomed. Mater. Res.

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A biophysical model linking fibrin polymerization kinetics (following release from a thrombin-fibrinogen complex), coagulation time, and competitive inhibition of thrombin illustrates the utility of thrombin-binding ligands as anticoagulants in blood collection applications. The resulting mathematical relationship connecting fibrinogen, ligand, and thrombin concentrations was tested against experimentally observed anticoagulation of whole, platelet-poor porcine plasma induced by short, single-stranded DNA oligonucleotides originally found to bind thrombin by screening combinatorial libraries. The thrombin-fibrinogen dissociation constant Ks served as the single adjustable parameter in a least-squares fitting of the model to experimental anticoagulation data. Best-fit Ks values corroborated microM values measured in plasma-free systems, and application of the model to a ligand challenge to the intrinsic pathway of plasma coagulation corroborated nM endogenous thrombin concentrations measured in porcine blood activated by endotoxin insult in vivo. The model fit to data suggests that only about 20% conversion of blood fibrinogen to fibrin is required to coagulate (gel) porcine plasma. This prediction is consistent with the common clinical laboratory observation of latent fibrin formation in "serum" separated from blood before fibrinogen is fully converted to fibrin. It was concluded that the thrombin-binding anticoagulation model was a reasonable simulation of the situation in which an initial bolus of either exogenous or endogenous thrombin is rapidly partitioned between fibrinogen-bound and ligand-bound forms with little or no additional free thrombin created over time.

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Section: Implications For Thrombin-binding Anticoagulants In Blood Comentioning

confidence: 99%

Contact activation of the plasma coagulation cascade. III. Biophysical aspects of thrombin-binding anticoagulants

Vogler

Nadeau

Graper

1998

J. Biomed. Mater. Res.

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“…Electrophoretic separation of plasma proteins into five major fractions was performed on cellulose ace tate strips (16). Densitometric quantitation of the electrophoretic pattern was carried out with an auto matic recording and integrating densitometer.…”

Section: Measurementsmentioning

confidence: 99%

Effects of Large Volume-Hemodilution Perfusion on Plasma Volume, Plasma Proteins and Plasma Electrolytes in Neonatal Piglets

LeGal¹,

Snellen²,

Littlefield³

1977

Neonatology

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Two groups of 15 neonatal piglets underwent arteriovenous perfusion with the chest closed, at normothermia and without an oxygenator in the system until complete mixing of the blood and priming fluid was accomplished. The extracorporeal circuit was primed in one group with a warm (38 °C) buffered Ringer-lactate solution, and in the second group with an equally warm buffered solution of 2% Rheomacrodex in Ringer-lactate. The priming volumes of the perfused animals were individually tailored to result in a mixed (blood and priming solution) hematocrit of 22%. A control group of 15 piglets underwent the same surgical procedures but were not perfused. Large vessel hematocrit, plasma volume, the five major plasma protein components, plasma electrolytes and plasma osmolarity were measured before perfusion, immediately after and every 8 h for a period of 24 h. During perfusion, plasma volume, erythrocytes, and proteins were lost from the circulation. Plasma osmolarities showed larger fluctuations in the Rheomacrodex group than in the Ringer-lactate group. The neonatal pig is able to cope with large volume hemodilution resulting in a hematocrit of 22%. It shows a well-developed regulatory mechanism to counteract plasma volume and plasma protein losses; the replenishment of plasma volume and restoration of each major circulating plasma protein fraction, except γ-globulin, are accomplished in about 24 h. The restoration of plasma volume occurs faster than the restoration of plasma proteins, but when dextran is present the reverse is the case. Since the restoration of plasma volume, plasma proteins, and red blood cells do not show the same patterns, sequestration of whole blood is unlikely. 2% Rheomacrodex in Ringer-lactate appears to enhance protein restoration considerably.

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Characterization and Quantitation of the Five Major Plasma Protein Fractions in Seven-Day-Old Piglets

Cited by 2 publications

References 6 publications

Contact activation of the plasma coagulation cascade. III. Biophysical aspects of thrombin-binding anticoagulants

Contact activation of the plasma coagulation cascade. III. Biophysical aspects of thrombin-binding anticoagulants

Effects of Large Volume-Hemodilution Perfusion on Plasma Volume, Plasma Proteins and Plasma Electrolytes in Neonatal Piglets

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