Characterization and regulation of Bacillus thuringiensis Cry toxin binding aminopeptidases N (APNs) from non-gut visceral tissues, Malpighian tubule and salivary gland: Comparison with midgut-specific APN in the moth Achaea janata

Ningshen, Thuirei Jacob; Chaitanya, R.K.; Hari, Prashanth P.; Devi, P. S. Vimala; Dutta-Gupta, Aparna

doi:10.1016/j.cbpb.2013.09.005

Cited by 11 publications

(5 citation statements)

References 58 publications

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“…Many of these proteins were reported as possible participants in the Cry mechanism of action, either by direct interaction with the toxin or by changing its expression levels in response to Bt infection. More than one APN for the same species was grouped among the eight classes, which could indicate that the protein belongs to a group of duplicated APN genes [ 122 ] or was derived from different tissues rather than just the midgut [ 123 ]. Class 1 presented proteins that were previously reported as Cry toxin receptors [ 31 ] as well as proteins identified in recent publications.…”

Section: Resultsmentioning

confidence: 99%

Sugarcane Giant Borer Transcriptome Analysis and Identification of Genes Related to Digestion

et al. 2015

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Sugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the GenBank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a number of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect’s biology and to guide the development of new strategies for insect-pest control.

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Section: Resultsmentioning

confidence: 99%

Sugarcane Giant Borer Transcriptome Analysis and Identification of Genes Related to Digestion

et al. 2015

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“…Membrane-bound aminopeptidases in the gut are receptors for Bacillus thuringiensis toxins in several insect species [ 112 – 114 ]. Aminopeptidases have also been detected in other insect tissues, such as the fat body [ 115 ], salivary glands [ 116 ], and Malpighian tubes [ 116 ]. Although their interactions with B. thuringiensis toxins have been confirmed, their endogenous role is unclear [ 116 ].…”

Section: Resultsmentioning

confidence: 99%

“…Aminopeptidases have also been detected in other insect tissues, such as the fat body [ 115 ], salivary glands [ 116 ], and Malpighian tubes [ 116 ]. Although their interactions with B. thuringiensis toxins have been confirmed, their endogenous role is unclear [ 116 ]. Aminopeptidase N in the hemocoel plays an important role in the postembryonic development of the pest moth Achaea janata [ 117 ].…”

Section: Resultsmentioning

confidence: 99%

Next Generation Sequencing Identifies Five Major Classes of Potentially Therapeutic Enzymes Secreted byLucilia sericataMedical Maggots

Franta

Vogel

Lehmann

et al. 2016

BioMed Research International

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Lucilia sericata larvae are used as an alternative treatment for recalcitrant and chronic wounds. Their excretions/secretions contain molecules that facilitate tissue debridement, disinfect, or accelerate wound healing and have therefore been recognized as a potential source of novel therapeutic compounds. Among the substances present in excretions/secretions various peptidase activities promoting the wound healing processes have been detected but the peptidases responsible for these activities remain mostly unidentified. To explore these enzymes we applied next generation sequencing to analyze the transcriptomes of different maggot tissues (salivary glands, gut, and crop) associated with the production of excretions/secretions and/or with digestion as well as the rest of the larval body. As a result we obtained more than 123.8 million paired-end reads, which were assembled de novo using Trinity and Oases assemblers, yielding 41,421 contigs with an N50 contig length of 2.22 kb and a total length of 67.79 Mb. BLASTp analysis against the MEROPS database identified 1729 contigs in 577 clusters encoding five peptidase classes (serine, cysteine, aspartic, threonine, and metallopeptidases), which were assigned to 26 clans, 48 families, and 185 peptidase species. The individual enzymes were differentially expressed among maggot tissues and included peptidase activities related to the therapeutic effects of maggot excretions/secretions.

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“…13 Studies have demonstrated that Cry toxins bind to in vitro APN on Malpighian tubules. 14 Malpighian tubules play a vital role in ensuring a constant internal environment through the excretion of unnecessary substances present in the hemolymph and the maintenance of useful substances to the insect. 15 Generally, the Malpighian tubules are inserted between the midgut and the hindgut, and divided into proximal and distal parts, which are responsible for fluid reabsorption and fluid excretion, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…Cry11Bb toxin is located on the apical microvilli of the Malpighian tubules of Culex quinquefasciatus larval after Cry toxin treatment 13 . Studies have demonstrated that Cry toxins bind to in vitro APN on Malpighian tubules 14 . Malpighian tubules play a vital role in ensuring a constant internal environment through the excretion of unnecessary substances present in the hemolymph and the maintenance of useful substances to the insect 15 .…”

Section: Introductionmentioning

confidence: 99%

Proteotranscriptomic analyses of the midgut and Malpighian tubules after a sublethal concentration of Cry1Ab exposure on Spodoptera litura

Xu,

Zhang,

Xue‐Yang

et al. 2024

Pest Management Science

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BACKGROUNDCry1Ab has emerged as a bio‐insecticide to control Spodoptera litura (Lepidoptera: Noctuidae). However, the sublethal effects of Cry1Ab on the physiological changes and molecular level of S. litura have not been well documented. Our aims in this study were to assess the sublethal effect of Cry1Ab on S. litura, including midgut and Malpighian tubules as targets.RESULTSAfter sublethal Cry1Ab exposure, distinct histological alterations were mainly observed in the midgut. Furthermore, the results of comparative RNA sequencing and tandem mass tag‐based proteomics showed that, in the midgut, most differential expression genes (DEGs) were up‐regulated and significantly enriched in the serine protease activity pathway, and up‐regulated differential expression proteins (DEPs) were mainly associated with the oxidative phosphorylation pathway, whereas the down‐regulated involved in the ribosome pathways. In the Malpighian tubules, DEGs and DEPs were significantly enriched in the ribosome pathway. We proposed that ribosome may act as a universal target in energy metabolism with other pathways via the results of protein–protein interaction analysis. Further, by verification of the mRNA expression of some Cry protein receptor and detoxification genes after Cry1Ab treatment, it was suggested that the ribosomal proteins (RPs) possibly participate in influencing the Bt‐resistance of S. litura larvae under sublethal Cry1Ab exposure.CONCLUSIONUnder sublethal Cry1Ab exposure, the midgut of S. litura was damaged, and the proteotranscriptomic analysis elucidated that Cry1Ab disrupted the energy homeostasis of larvae. Furthermore, we emphasized the potential role of ribosomes in sublethal Cry1Ab exposure. © 2024 Society of Chemical Industry.

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Characterization and regulation of Bacillus thuringiensis Cry toxin binding aminopeptidases N (APNs) from non-gut visceral tissues, Malpighian tubule and salivary gland: Comparison with midgut-specific APN in the moth Achaea janata

Cited by 11 publications

References 58 publications

Sugarcane Giant Borer Transcriptome Analysis and Identification of Genes Related to Digestion

Sugarcane Giant Borer Transcriptome Analysis and Identification of Genes Related to Digestion

Next Generation Sequencing Identifies Five Major Classes of Potentially Therapeutic Enzymes Secreted byLucilia sericataMedical Maggots

Proteotranscriptomic analyses of the midgut and Malpighian tubules after a sublethal concentration of Cry1Ab exposure on Spodoptera litura

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