Characterization and Screening of Thermophilic Bacillus Strains for Developing Plant Growth Promoting Consortium From Hot Spring of Leh and Ladakh Region of India

Abstract

In the present investigation, the main aim is to identify and characterize the potential drought tolerant plant growth promoting consortium for agricultural productivity. Three bacterial isolates were isolated from hot spring of Chumathang area of Leh district. Bacillus species (BHUJP-H1, BHUJP-H2, and BHUJP-H3) were done some biochemical tests including catalase, cellulase, amylase, indole-3-acetic acid, phosphate solubilisation, production of ammonia, siderophore, and hydrogen cyanide. Molecular characterization of isolates was done by 16S rDNA sequencing, e.g., Bacillus subtilis BHUJP-H1 (KU312403), Bacillus sp. BHUJP-H2 (KU312404) and B. licheniformis BHUJP-H3 (KU312405). The genetic diversity of the isolates was assessed by seven inter simple sequence repeat, all primer shows high polymorphism. The highest polymorphism efficiency and polymorphism information content showed by UBC-809 and UBC-836 which were 100% and 0.44 respectively, the lowest is by UBC-807 75% and 0.28 respectively. On an average 90.69% polymorphism efficiency and 0.40 polymorphism information contents obtained by used markers. The highest, 11.08 and the lowest, 4.50 effective multiplex ratios obtained for primer UBC-823 and UBC-807, on an average 7.99 effective multiplex ratio obtained. The highest, 4.89 and the lowest, 1.25 marker indexes obtained by UBC-836 and UBC-807 respectively and on an average 3.24 obtained. The UPGMA cluster analysis divided a population into two clusters I and II, in which BHUJP-H1 and BHUJP-H2 grouped under same while BHUJP-H3 grouped under another cluster. The treatment combination of Bacillus subtilis BHUJP-H1, B. subtilis BHUJP-H1+ B. licheniformis BHUJP-H3 and B. subtilis BHUJP-H1+ Bacillus sp. BHUJP-H2+ B. licheniformis BHUJP-H3 were recorded better combination for enhancing plant growth attributes of Vigna radiata as compared to control and others. The plant growth promoting consortium, e.g., Bacillus subtilis BHUJP-H1, Bacillus subtilis BHUJP-H1+ B. licheniformis BHUJP-H3 and B. subtilis BHUJP-H1+ Bacillus sp. BHUJP-H2+ B. licheniformis BHUJP-H3 can be further used as effective microbial inoculant for enhancing the production of mungbean in field conditions. Bacillus sp. BHUJP-H1 and Bacillus sp. BHUJP-H2 may use as drought tolerant plant growth promoting consortium for enhancing the sustainable agricultural productivity.

Identification of New Biocontrol Agent against Charcoal Rot Disease Caused by Macrophomina phaseolina in Soybean (Glycine max L.)

“…In our study, we isolated bacterial strains from hot springs, screened them for plant growth promotion properties, and applied them on charcoal rot susceptible soybean variety NARC-II to observe their effects on disease control by modulating ROS scavenging system in soybean plants. Plant growth promoting bacteria (PGPB) isolated from hot springs and their vicinities are reported to be efficient plant growth-promoting agents [37,38]. In this study, 20 bacterial strains were isolated.…”

Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues

“…The cellulolytic enzymes work efficiently at temperatures around 50 • C and in some studies, even at higher temperatures (Baharuddin et al, 2016). Verma et al (2018) isolated the cellulase producing thermophilic Bacillus subtilis BHUJP-H1, Bacillus sp. BHUJP-H2, Bacillus licheniformis BHUJP-H3 from hot spring of Leh and Ladakh, indicates that these samples would provide bacteria inhabiting extreme environment, high temperature, and pressure tolerance (high altitude).…”

Salt tolerance enhancement Of wheat <em>(Triticum Asativium L)</em> genotypes by selected plant growth promoting bacteria

“…Pseudomonas have been widely reported as NFB of nonlegumes [38] , [39] . Previous studies have shown that plant growth promoting bacteria are able to not only fix nitrogen but also produce IAA and ACC deaminase [40] , [41] .…”