Characterization and selection of rhizobia for use as inoculants for groundnuts in Sudan

Hadad, M. A.

doi:10.31274/rtd-180813-5611

Cited by 2 publications

(3 citation statements)

References 31 publications

(45 reference statements)

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“…Thus not only R. japonicum, but also cross-inoculation was absent, confirming the findings of Habish & Khairi (1968). The red to pink nodule inside indioated good N 2 -fixing aotivity (Hadad, 1980). The low nodulation could account for there being no significant differences in shoot dry matter between treatments at 4 weeks (S^ although there were marked effects at 60 % flowering.…”

Section: Discussionmentioning

confidence: 92%

Inoculation of irrigated soya bean in the Sudan Gezira

Mukhtar

Naib

1987

J. Agric. Sci.

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Soya bean variety Semmes failed to nodulate with a number of R.japonicum strains. Some of these were applied to the seed in a carrier based on Nile silt.A three-strain composite in peat gave good nodulation and reasonable N fixation. Inoculation significantly outyielded 100 kg N/ha added as urea in three equal portions at sowing, 1 and 2 months after sowing. Plant dry matter, nodule dry matter, yield, yield components and seed protein were also significantly enhanced by inooulation.Phosphorus applied at 50 and 100 kg P 2 O 6 /ha in combination with a starter dose of nitrogen (10 kg/ha) with inoculation both gave erratic and inconclusive results.

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Section: Discussionmentioning

confidence: 92%

Inoculation of irrigated soya bean in the Sudan Gezira

Mukhtar

Naib

1987

J. Agric. Sci.

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“…Among the commercial inoculants, biofix beans was the best by 153% relative effective than some other isolates. Isolates also can also support growth of rhizobia and successful nodulation in legumes (Argaw, 2012;Hadad, 1984;Onyango et al, 2015). The process such as leaching of N and P adsorption due to the low pH could influence low level of these nutrients (Mowo et al, 2006).…”

Section: Relative Symbiotic Effectivenessmentioning

confidence: 99%

“…Inorganic fertilizers are commonly used to overcome the situation but are not accessible to most of African farmers, because it they are expensive (Laurette et al, 2015). Planting legumes either by crop rotation or intercropping with some cereal crops, has become an important and affordable way of improving soil condition and nutrients (Hadad, 1984). Legumes has been reported to facilitate in BNF, soil cover and as source of organic matter in the soils (Grönemeyer et al, 2017;Simon et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Characterization and effectiveness determination of indigenous rhizobia nodulating lablab (lablab purpureus) in Babati district, Tanzania

Malugu

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The soil samples from fifteen villages which grow Lablab in Babati district were collected and used for planting Lablab in experimental pot to trap indigenous rhizobia. At a flowering stage, the plants were harvested and root nodules were collected for rhizobia isolation using Yeast Extract Mannitol Agar (YEMA). Under aseptic conditions, twenty-two isolates were obtained and characterized by morphological, biochemical and physiological characteristics. Furthermore, PCR amplification of 16S rDNA and symbiotic effectiveness were also evaluated. The results showed that, rhizobia were fast and slow growing in YEMA after 4 - 7 days of incubation. In YEMA with Congo red one isolates absorbed the color, while, physiologically all isolates were alkaline tolerant, however susceptible to acidity and sodium chloride above 3%. High diversity among isolates was observed in dendrogram at 70% similarity level by forming two groups with seven clusters. Only 18 isolates out of 22 were amplified. In testing symbiotic effectiveness, all 22 isolates induced nodules in Lablab, interestingly some were effective than commercial inoculants. Most of isolates recorded relative symbiotic effectiveness above 100, the highest was 193 for BR4. Isolates BR4, BR13, BR16, BR5 and BR18 were effective than other isolates and commercial strains. The results implied presence of diverse and effective indigenous rhizobia nodulating Lablab in Tanzania, also highlights their potential to be used for inoculating Lablab instead of commercial inoculants. To recommend characterization by sequencing to identify their genetic groups and field effectiveness evaluation is needed in order to identify more effective isolates for inoculants production.

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Characterization and selection of rhizobia for use as inoculants for groundnuts in Sudan

Cited by 2 publications

References 31 publications

Inoculation of irrigated soya bean in the Sudan Gezira

Inoculation of irrigated soya bean in the Sudan Gezira

Characterization and effectiveness determination of indigenous rhizobia nodulating lablab (lablab purpureus) in Babati district, Tanzania

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