Characterization and selection ofBacillus sp. strains, effective biocontrol agents againstFusarium oxysporum f. sp.radicis-lycopersici, the causal agent of Fusarium crown and root rot in tomato

Saidi, Nurdin; Kouki, Soulwène; Mhiri, F.; Hajlaoui, Mohamed Rabeh; Mahrouk, Meriam; Ouzari, Hadda; Jedidi, Naceur; Hassen, Abdennaceur

doi:10.1007/bf03178317

Cited by 18 publications

(13 citation statements)

References 41 publications

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“…Several Bacillus species have demonstrated antifungal activity against fungal phytopathogens, including B. cinerea and/or Fusarium species, in vitro [39][40][41][42][43]. However, antifungal capability is a function of bacterial strain and fungal pathogen.…”

Section: Bacillus Speciesmentioning

confidence: 99%

“…The application of Bacillus species pre-harvest reduced the incidence and severity of diseases caused by F. oxysporum [42], Alternaria solani, and Phytophthora infestans [44] on tomato plants and B. cinerea fruit rot on strawberry plants [29]. Postharvest application of Bacillus species has also caused fungal suppression; decay was reduced on pears, tomatoes, bananas, and oranges [12,31,32,45].…”

Section: Bacillus Speciesmentioning

confidence: 99%

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Antifungal Screening of Bioprotective Isolates against Botrytis cinerea, Fusarium pallidoroseum and Fusarium moniliforme

Senna

Lathrop

2017

Fermentation

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Abstract:The fungi Botrytis cinerea, Fusarium pallidoroseum, and Fusarium moniliforme are the causative agents of several plant diseases and can cause significant crop loss both before and after harvest. Fungicides are employed to control these phytopathogens, but fungicide use has led to an increase in resistance and may negatively affect the environment and human health. Hence, more environmentally sustainable solutions such as biological control methods are needed. The purpose of this study was to screen 22 bacterial isolates for inhibitory activity against fungal phytopathogens. To evaluate antifungal activity, the bacterial isolates were individually spot-inoculated onto Tryptic Soy Agar or de Man, Rogosa, Sharpe agar, and then a plug of fungal-colonized agar was placed onto the center of the isolate-inoculated plate. Plates were incubated at 24 • C for 10 days and fungal growth was evaluated. Nine of the 22 isolates screened inhibited all three fungi; inhibition by these isolates ranged from 51-62%, 60-68%, and 40-61% for B. cinerea, F. pallidoroseum, and F. moniliforme, respectively. Isolates were also screened for biosurfactant activity using the drop-collapse test. Bacillus megaterium, Bacillus coagulans, Bacillus thuringiensis and three Bacillus amyloliquefaciens isolates demonstrated strong biosurfactant activity and suppression of all three fungi, and therefore are recommended for further study.

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Section: Bacillus Speciesmentioning

confidence: 99%

Section: Bacillus Speciesmentioning

confidence: 99%

Antifungal Screening of Bioprotective Isolates against Botrytis cinerea, Fusarium pallidoroseum and Fusarium moniliforme

Senna

Lathrop

2017

Fermentation

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“…Some authors found that production of pyoverdines contributes to the bio-control capacity of the fluorescent Pseudomonas. Conversely, other studies failed to establish a link between production of pyoverdine and antagonism against phytopathogenic fungi Kumar et al,2008;Saidi et al, 2009;Yang et al, 2009. Pseudomonas spp. has been shown to produce siderophores able to chelate any available iron (Henry et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Effect of Dose-Response of Zinc and Manganese on Siderophores Production

Mehri¹,

Khessairi²,

Turki³

et al. 2012

American Journal of Environmental Sciences

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Problem statement:This study was conducted to find and determine whether the siderophores of the four environmental Pseudomonas spp. isolates possess a sequestering activity towards essential transition metals (Zn and Mn) other than iron. Approach: Four fluorescent Pseudomonads isolated from various environments, were characterized analytically (Isoelectric focusing), biologically (pyoverdine-mediated uptake) and genetically (16S rDNA sequencing). By means of spectrophotometric measurements, it was possible to establish and compare the levels of pyoverdine production, in two different nutrient-poor media. Results: The strains were assigned, by sequencing, to P. fluorescens, P. aeruginosa, P. putida and P. mosselii isolated, respectively from soil, compost, sea water and waste water treatment plant. These bacterial strains were recognized as producing diver's yellow-green siderophores types, when grown under conditions of iron starvation. The highest metabolite concentration was obtained with PsC132 and PsTp171 strains isolated respectively from compost and waste water treatment plant, in CAA medium. Strains grown in CAA medium exhibit a higher PVD level compared to SM medium. Mn (II) was found to promote pyoverdine biosynthesis, but rather, Zn (II) had no significant effect on siderophore production when compared to control medium. For both strains PsS29 and PsC132, the increase of iron concentration quenched siderophore production especially above 20 µM. Pyoverdine level declined with the high concentration of zinc but increased with Manganese concentration ranging up to 70 µM (in case of PsC132) and 300 µM (in case of PsS29). Conclusion/Recommendations: The ability of fluorescent Pseudomonas, isolated from wastewater treatment plant and from compost, to sequester zinc, point to a unique advantage of these species for divers bioremediation applications.

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“…The development of biological controls has recently received attention because of their economic and ecological advantages, as well as political demands. Several studies have examined biological control approaches against the disease (6, 28, 31, 35). In Japan, Iwamoto and Aino (20) reported that a commercial biocontrol agent (an endophytic bacterium of tomato roots, Pseudomonas fluorescens FPH9601) significantly suppressed the disease in tomato fields, although the sale of the agent is currently suspended.…”

mentioning

confidence: 99%

Suppressive Potential of <i>Paenibacillus</i> Strains Isolated from the Tomato Phyllosphere against Fusarium Crown and Root Rot of Tomato

et al. 2014

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The suppressive potentials of Bacillus and Paenibacillus strains isolated from the tomato phyllosphere were investigated to obtain new biocontrol candidates against Fusarium crown and root rot of tomato. The suppressive activities of 20 bacterial strains belonging to these genera were examined using seedlings and potted tomato plants, and two Paenibacillus strains (12HD2 and 42NP7) were selected as biocontrol candidates against the disease. These two strains suppressed the disease in the field experiment. Scanning electron microscopy revealed that the treated bacterial cells colonized the root surface, and when the roots of the seedlings were treated with strain 42NP7 cells, the cell population was maintained on the roots for at least for 4 weeks. Although the bacterial strains had no direct antifungal activity against the causal pathogen in vitro, an increase was observed in the antifungal activities of acetone extracts from tomato roots treated with the cells of both bacterial strains. Furthermore, RT-PCR analysis verified that the expression of defense-related genes was induced in both the roots and leaves of seedlings treated with the bacterial cells. Thus, the root-colonized cells of the two Paenibacillus strains were considered to induce resistance in tomato plants, which resulted in the suppression of the disease.

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Characterization and selection ofBacillus sp. strains, effective biocontrol agents againstFusarium oxysporum f. sp.radicis-lycopersici, the causal agent of Fusarium crown and root rot in tomato

Cited by 18 publications

References 41 publications

Antifungal Screening of Bioprotective Isolates against Botrytis cinerea, Fusarium pallidoroseum and Fusarium moniliforme

Antifungal Screening of Bioprotective Isolates against Botrytis cinerea, Fusarium pallidoroseum and Fusarium moniliforme

Effect of Dose-Response of Zinc and Manganese on Siderophores Production

Suppressive Potential of <i>Paenibacillus</i> Strains Isolated from the Tomato Phyllosphere against Fusarium Crown and Root Rot of Tomato

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