Characterization and sensitivity to antibiotics of bacteria isolated from the lower respiratory tract of ventilated patients hospitalized in intensive care units

Medell, Manuel; Medell, Mariana; Martínez, Alicia; Valdés, Rodolfo

doi:10.1016/s1413-8670(12)70273-8

Cited by 16 publications

(3 citation statements)

References 25 publications

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“…Different studies have reported different values for the cytotoxicity of probiotics. Shahid et al reported a cytotoxicity rate of approximately 28%, which was higher than that of the present study [ 34 ], whereas other studies found a cytotoxicity rate of less than 10% [ 35 , 36 ].…”

Section: Discussioncontrasting

confidence: 90%

“…On the other hand, L. rhamnosus strains were reported as potentially useful probiotic bacteria against these pathogenic strains. Similar to the previous studies, P. aeruginosa strains were frequently isolated from respiratory samples [ 36 ]. Ahmed et al reported that most cases of isolated P. aeruginosa were detected in respiratory samples.…”

Section: Discussionsupporting

confidence: 76%

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Investigating the Antagonistic Effect of Indigenous Probiotics on Carbapenem‐Resistant Pseudomonas aeruginosa Strains

Ghiaei,

Ghasemi,

Shokri

2023

BioMed Research International

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Introduction. With the increase of hospital infections due to the indiscriminate use of antibiotics, multidrug resistance has increased, decreasing the effectiveness of antibiotics against these infections. For this reason, the identification of alternative agents such as probiotics has been considered. The aim of this study was to isolate and identify effective probiotics against carbapenem-resistant Pseudomonas aeruginosa strains. Material and Methods. During a period of eight months, isolates of P. aeruginosa were collected from patients in three hospitals in Isfahan. The presence of metallo-beta-lactamase enzymes was determined by the combination disc test (CDT). The inhibitory and antimicrobial activities of 20 probiotic bacteria isolated from local dairy products against these strains were investigated by agar dilution. Two probiotic strains that showed broader inhibition results were selected, and the values of the lowest inhibitory concentration (MIC) and the lowest lethal concentration (MBC) and their antibiofilm effect were determined using the microtiter plate method. The concentration of organic acids was done by HPLC. Findings. Of the 100 samples isolated and identified, 61 samples (61%) exhibited multiple drug resistance (MDR) and were selected for further investigation. Phenotypic diagnosis of the presence of metallo-beta-lactamase enzymes revealed that 74.5% of the strains were positive. The results showed that these two probiotics killed P. aeruginosa strains after only one hour, and the inhibition mechanism was due to the presence of lactic acid and acetic acid. The antibiofilm effect of these two probiotics was at concentrations of 1/2 and 1/4. Conclusion. The two Lactobacillus isolates had potential antimicrobial and antibiofilm properties against all carbapenem-resistant P. aeruginosa strains, even at thinner dilutions. Considering the broad activity of this strain, it can potentially be used for biocontrol of these strains.

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Section: Discussioncontrasting

confidence: 90%

Section: Discussionsupporting

confidence: 76%

Investigating the Antagonistic Effect of Indigenous Probiotics on Carbapenem‐Resistant Pseudomonas aeruginosa Strains

Ghiaei,

Ghasemi,

Shokri

2023

BioMed Research International

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“…Superinfections in combination with viral respiratory infections are commonly caused by the above-mentioned microorganisms in patients hospitalized or maintained on mechanical ventilation for a prolonged period [11]. Underlying comorbidities are also a risk factor for co-infections among people of all age groups [12, 13].…”

Section: Introductionmentioning

confidence: 99%

Respiratory bacterial co-infections and their antibiotic resistance pattern in COVID-19 patients at a tertiary care centre in India

Kar

Siddiqui

Dubey

et al. 2023

Access Microbiology

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Introduction. Patients with coronavirus disease-2019 (COVID-19) are prone to develop respiratory bacterial infections irrespective of their need for mechanical ventilatory support. Hypothesis/Gap Statement. Information about the incidence of concomitant respiratory bacterial infections in COVID- 19 patients from India is limited. Aim. This study aimed to determine the incidence of concomitant respiratory bacterial pathogens and their drug resistance in these patients. Methodology. A prospective study was performed by including patients who were admitted to our tertiary care centre from March 2021 to May 2021 to evaluate secondary bacterial respiratory co-infections in patients via real-time PCR (RT-PCR)-confirmed cases of COVID-19 disease caused by SARS CoV-2. Results. Sixty-nine culture-positive respiratory samples from patients with COVID-19 were incorporated into this study. The most commonly isolated bacterial microorganisms were Klebsiella pneumoniae (23 samples, 33.33 %) and Acinetobacter baumannii (15, 21.73 %), followed by Pseudomonas aeruginosa (13, 18.84 %). Among the microorganisms isolated, 41 (59.4 %) were multidrug-resistant (MDR) and nine (13 %) were extensively drug-resistant (XDR). Among the Gram-negative bacteria isolated, K. pneumoniae showed high drug resistance. Fifty carbapenem-resistant microorganisms were isolated from the patients included in our study. Concerning the hospital stay of the patients enrolled, there was an increased length of intensive care unit stay, which was 22.25±15.42 days among patients needing mechanical ventilation in comparison to 5.39±9.57 days in patients on ambient air or low/high-flow oxygen. Conclusion. COVID-19 patients need increased length of hospitalization and have a high incidence of secondary respiratory bacterial infections and high antimicrobial drug resistance.

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COVID‐19: Inpatient Management

Lopez

Matusov

Pedraza

et al. 2022

Coronavirus Disease 2019 (COVID‐19)

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Characterization and sensitivity to antibiotics of bacteria isolated from the lower respiratory tract of ventilated patients hospitalized in intensive care units

Cited by 16 publications

References 25 publications

Investigating the Antagonistic Effect of Indigenous Probiotics on Carbapenem‐Resistant Pseudomonas aeruginosa Strains

Investigating the Antagonistic Effect of Indigenous Probiotics on Carbapenem‐Resistant Pseudomonas aeruginosa Strains

Respiratory bacterial co-infections and their antibiotic resistance pattern in COVID-19 patients at a tertiary care centre in India

COVID‐19: Inpatient Management

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