Characterization and validation of bovine Gonadotripin releasing hormone receptor (GNRHR) polymorphisms

Lirón, Juan Pedro; Prando, Alberto José; Ripoli, María Verónica; Rogberg-Muñoz, A.; Posik, Diego Manuel; Baldo, Andrés; Peral‐García, Pilar; Giovambattista, Guillermo

doi:10.1016/j.rvsc.2010.09.024

Cited by 13 publications

(9 citation statements)

References 49 publications

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“…The later age at first calving is assumed to be due to a later age at puberty; however, in the present study age at puberty was not determined. The C allele of the GnRHR that had a greater frequency in Bos indicus than Bos taurus cattle in a previous study (Lirón et al, 2011) was associated with an increased age at first calving in homozygous cows in the present study. Because calving early in the first calving season is associated with improved lifetime productivity (Burris and Priode, 1958;Lesmeister et al, 1973), this polymorphism may be useful for selecting heifers that will conceive early and remain in the production herd longer.…”

Section: Discussionsupporting

confidence: 62%

“…An assay was designed to genotype these GRIA1 and GnRHR polymorphisms using primer extension assays on a Sequenom MassArray analyzer (Sequenom, San Diego, CA). The GnRHR polymorphism was included in the assay because it was proposed as a marker of fertility and age at puberty because of the greater frequency of the C allele in Bos indicus cattle compared with Bos taurus cattle (Lirón et al, 2011).…”

Section: Genotypingmentioning

confidence: 99%

“…Secondarily, we examined the influence of a polymorphism in the GnRH receptor (GnRHR) on reproductive traits in repeat breeder cows. This GnRHR polymorphism was investigated because it was proposed as a genetic marker of fertility and age at puberty because of the greater frequency of the C allele in Bos indicus cattle compared with Bos taurus cattle (Lirón et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Identification of an ionotropic glutamate receptor AMPA1/GRIA1 polymorphism in crossbred beef cows differing in fertility12

Cushman

Miles

Rempel

et al. 2013

Journal of Animal Science

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ABSTRACT:A proposed functional polymorphism in the ionotropic glutamate receptor AMPA1 (GRIA1) has been reported to influence antral follicle numbers and fertility in cows. Repeat breeder cows that fail to produce a calf in multiple seasons have been reported to have reduced numbers of small (1 to 3 mm) antral follicles in their ovaries. Therefore, we tested the hypothesis that this GRIA1 polymorphism was affecting antral follicle numbers in repeat breeder cows. Repeat breeder cows (n = 64) and control cows (n = 72) that had always produced a calf were housed in a dry lot and observed twice daily for behavioral estrus. Blood samples were collected, and cows were genotyped for this GRIA1 polymorphism and for a polymorphism in the GnRH receptor (GnRHR) that was proposed to influence age at puberty. On d 3 to 8 after estrus cows were slaughtered, and reproductive organs were collected to determine antral follicle count, ovary size, and uterine horn diameter. Repeat breeder cows were older at first calving than control cows (P = 0.006). The length (P = 0.03) and height (P = 0.02) of the ovary contralateral to the corpus luteum (CL) were greater in control cows than repeat breeder cows. The endometrial diameter in the horn ipsilateral to the CL was greater in the control cows than the repeat breeder cows. Repeat breeder cows had fewer small (1 to 5 mm) antral follicles than control cows (P = 0.003); however, there was no association between GRIA1 genotype and antral follicle number. The GnRHR polymorphism was associated with age at first calving because cows that were homozygous for the C allele had a greater age at first calving than heterozygous cows or cows that were homozygous for the T allele (P = 0.01). In the granulosa cells from small (1 to 5 mm) antral follicles, mRNA abundances of 2 markers of oocyte quality, anti-Müllerian hormone and pentraxin 3, did not differ between fertility groups (P ≥ 0.12). We conclude that this GRIA1 polymorphism exists in beef cows but that it does not influence antral follicle numbers. The association between GnRHR genotype and age at first calving is likely not causal as this polymorphism is not functional. The utility of this polymorphism as a genetic marker for early conception in heifers will require further validation. Screening postpartum cows by ultrasonography to determine antral follicle numbers may aid in making culling decisions.

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Section: Discussionsupporting

confidence: 62%

Section: Genotypingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of an ionotropic glutamate receptor AMPA1/GRIA1 polymorphism in crossbred beef cows differing in fertility12

Cushman

Miles

Rempel

et al. 2013

Journal of Animal Science

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“…Li et al (2011) showed that the polymorphisms detected by PCR-SSCP and DNA sequencing methods of the GnRHR gene were significantly associated with litter size in Shaanan and Boer goats. In the current study, allele-frequency analysis of genetic polymorphism patterns of GnRHR gene locus in investigated buffaloes (Table 1) revealed that pattern I (AA) was the most encountered compared with patterns II (BB) and III (CC) (51.7% vs. 44.1% and 4.3%, respectively).Similarly, Liron et al (2011) noticed lower frequencies of the C allele in European Bostaurus than B. indicus, meanwhile intermediate frequency of GnRHR alleles was recorded in Creole and Wagyu breeds. The ultrasound findings were used as the phenotype information to classify the animals into fertile and infertile animals.…”

Section: Resultsmentioning

confidence: 49%

“…Moreover, Yang et al (2011) reported that A-or C alleles had favorable positive effects on sperm quality traits in bulls. In bovine, Liron et al (2011) reported that European Bostaurus had lower frequencies of the C allele than B. indicus, while Creole and Wagyu breeds had intermediate frequency.…”

mentioning

confidence: 99%

Single Nucleotide Polymorphisms of GnRHR Gene and Its Relationship with Reproductive Performance in Egyptian Buffaloes

Sosa

M.²,

Eldebaky

et al. 2016

NIDOC-ASRT

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HIS WORK aimed to search for single nucleotide polymorphisms of gonadotropin releasing hormone receptor (GnRHR) gene in Egyptian buffalo which involved in endocrine control of fertility. An ultrasonographic examinations of anestrum and repeat breeders' buffaloes were performed prior to blood sampling through a transrectal ultrasonography. DNA was extracted from blood and the PCR and singlestrand conformation polymorphism (SSCP) techniques were adopted to study of GnRHR gene polymorphism. The PCR amplified a fragment with 240-bp in size and the SSCP results showed that there is a genetic polymorphism with three different patterns (AA, BB and CC) in Egyptian buffalo. The CC genotype was associated with smooth inactive ovary, while BB genotype was associated with fertility in buffalo. Multiple sequence alignment of the three patterns sequences revealed that GnRHR had 5 single nucleotide polymorphisms including one nucleotide insertion, one nucleotide deletion and 3 nucleotide substitutions. Insertion was at position 4 in pattern III. The deletion was at position 189 in pattern III, while the 3 nucleotide substitutions were at positions 204 (T/G), 206 (T/A) and 207 (A/T). In conclusion, GnRHR gene could be used as a candidate marker for fertility in Egyptian buffaloes with its mutation is related to ovarian inactivity.

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Incorporation of Genetic Technologies Associated with Applied Reproductive Technologies to Enhance World Food Production

Cushman

McDaneld

Kuehn

et al. 2013

Current and Future Reproductive Technologies and World Food Production

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Animal breeding and reproductive physiology have been closely related throughout the history of animal production science. Artificial insemination provides the best method of increasing the influence of sires with superior genetics to improve production traits. Multiple ovulation embryo transfer (MOET) provides some ability to increase the genetic influence of the maternal line as well. The addition of genetic technologies to this paradigm allows for improved methods of selecting sires and dams carrying the best genes for production and yield of edible products and resistance to diseases and parasites. However, decreasing the number of influential parents within a population also increases the risk of propagating a recessive gene that could negatively impact the species (Reprod Domest Anim 44:792-796, 2009; BMC Genomics 11:337, 2010). Furthermore, antagonistic genotypic relationships between production traits and fertility (Anim Prod Sci 49:399-412, 2009; Anim Genet 43:442-446, 2012) suggest that care must be taken to ensure that increasing the frequency of genes with a positive influence on production does not negatively impact the fertility of the replacement females entering the herd.

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Characterization and validation of bovine Gonadotripin releasing hormone receptor (GNRHR) polymorphisms

Cited by 13 publications

References 49 publications

Identification of an ionotropic glutamate receptor AMPA1/GRIA1 polymorphism in crossbred beef cows differing in fertility12

Identification of an ionotropic glutamate receptor AMPA1/GRIA1 polymorphism in crossbred beef cows differing in fertility12

Single Nucleotide Polymorphisms of GnRHR Gene and Its Relationship with Reproductive Performance in Egyptian Buffaloes

Incorporation of Genetic Technologies Associated with Applied Reproductive Technologies to Enhance World Food Production

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