Characterization, Antimicrobial and Antioxidant evaluation of biofabricated silver nanoparticles from endophytic Pantoea anthophila

Abstract

Endophyte mediated nanoparticles fabrication was emerging as a new frontier in nanomedicines that produce high biocompatible and functionalized silver nanoparticles. In this study, silver nanoparticles were successfully biosynthesized from the extracellular extract of endophytic bacterium Pantoea anthophila isolated from the stem of Waltheria indica for the first time. The synthesised nanoparticles were characterized by UV-Visible and Fourier Transform Infra-Red spectroscopy. The structural analysis is done by X-ray diffraction and the stability was studied by dynamic light scattering and particle size analyser. The size and shape were observed by Transmission Electron Microscope, Scanning Electron Microscope and Energy Dispersive X-Ray spectrum. Further, the nanoparticles were evaluated for antimicrobial and antioxidant properties. Synthesized nanoparticle showed a strong absorption band in the UV-Visible range at 410 nm. The average particle size was found to be 16.8 nm with spherical shaped, crystalline nature. Good zones of inhibition at various ranges were detected against a broad range of human pathogenic bacteria and fungi. A strong free radical scavenging activity of silver nanoparticles with IC50 values 30.75, 19.47, 34.59, 41.12, 27.24, 28.16, 36.21 µg/ml was obtained that was comparable to the reference. The study suggests that the silver nanoparticles can be biosynthesised from endophytic P. anthophila metabolites with significant therapeutic potential. With proper validation, the biosynthesised silver nanoparticles can be developed as a promising antiviral and anticancer drug candidate.

Lagenaria siceraria peel waste aqueous extract mediated silver nanoparticles for degradation of Congo red dye

“…No agglomeration was observed in TEM analysis of the sample. Similar results were reported in silver nanoparticles synthesis from endophytic Pantoea anthophila [38] and from seed extract of Tectona grandis [39].…”

“…The crystallite size of the silver nanoparticles synthesized from aqueous peel extract of Lagenaria siceraria was found to be much smaller than the crystallite size reported in silver nanoparticles synthesized from leaf extract of Carissa carandas [37] and in agreement with the crystallite size of particles synthesized from endophytic Pantoea anthophila [38]. This showed the significance of Lagenaria siceraria peel extract mediated silver nanoparticles over other biological methods.…”