Characterization, antimicrobial and antitumor activity of superoxide dismutase extracted from Egyptian honeybee venom (Apis mellifera lamarckii)

Abdel-Monsef, Mohamed M.; Darwish, Doaa A.; Zidan, Hind A.; Hamed, Ahmed A.; Ibrahim, Mona E.

doi:10.1186/s43141-023-00470-4

Cited by 5 publications

(4 citation statements)

References 58 publications

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“…After incubation, the positive antibacterial effect of the pigment was observed as clearance in the wells, while compounds that didn't influence the bacteria appeared opaque in the wells. The absorbance was measured after 20 h at OD 600 in a Spectrostar Nano Microplate Reader (BMG LABTECH GmbH, Allmendgrun, Germany) [26], [27].…”

Section: Antimicrobial Activity Of Yellow Pigment Extractmentioning

confidence: 99%

Bio-production and characterization of carotenoid yellow pigment from Kocuria sp. GMA and exploring its sustainable antioxidant, antimicrobial and antibiofilm properties

Ibrahim,

El-Shall,

Arafa

et al. 2024

Egypt. J. Chem.

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Bacterial pigments particularly carotenoid provide interesting prospects for a range of applications as antioxidants, antibacterial, and food additives. In-addition they are regarded as a competitive substitute for natural color production due to their better biodegradability and higher compatibility with the environment. This study aims to investigate the processes involved in producing carotenoid yellow pigment (CYP), including strain isolation and identification, pigment extraction, chemical characterization of the pigment, and testing of the pigment's antimicrobial, antioxidant, and antibiofilm properties. Using morphological traits and 16S rRNA sequencing, the isolate was recognized as Kocuria sp. GMA, with accession number OM921388. CYP from Kocuria sp .first, FTIR, LC-ESI-MS/MS spectrophotometers, and UV absorption spectra were used to chemically characterize CYP from Kocuria sp. GMA. In the pigment extract of Kocuriasp. bacteria, seven compounds, mostly carotenoids, were identified in the obtained LC-MS spectra: 224.04, 536.05, 553.22, 540.17, and 704.27, which correspond to a molecular weight of 224, 536, 552, 538.9, and 704 g/mol, which refer to Kocumarin, Lycopene, betacryptoxanthin, Neurosporene, and Flavuxanthin, respectively; peaks at m/z [M-H] lead to a molecular weight of 705.67 and 223.06, which refer to Kocumarin and Sarcinaxanthin, respectively. CYP possesses highly antioxidant activity (95.6%); this activity increased gradually with increasing concentrations and time, with an IC50 of 4.0 mg/ml at 90 min and 6.0 mg/ml at 60 min. Also, CYP showed moderate antimicrobial activity against the test pathogens with different concentrations, while it showed excellent activity as an antifungal against Aspergillus niger NRRLA-326. The MIC and MBC values of CYP against Gram-positive and Gram-negative bacteria ranged from 10 to 50 µg/ml. Results showed great antibiofilm activity of CYP against S. aureus NRRLB-767 and moderate activity against E. Coli ATCC 25922.

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Section: Antimicrobial Activity Of Yellow Pigment Extractmentioning

confidence: 99%

Bio-production and characterization of carotenoid yellow pigment from Kocuria sp. GMA and exploring its sustainable antioxidant, antimicrobial and antibiofilm properties

Ibrahim,

El-Shall,

Arafa

et al. 2024

Egypt. J. Chem.

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“…This is due to the well-documented diverse biological activity of this most expensive bee product. In particular, evidence has been obtained of its antimicrobial action against both bacteria (including antibiotic-resistant strains) and pathogenic fungi [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. The therapeutic effect of the external use of apitoxin in the treatment of wounds [ 24 , 25 ] as well as skin diseases [ 26 ] is largely associated with antimicrobial activity.…”

Section: Introductionmentioning

confidence: 99%

“…Both BV as a whole and its individual components demonstrate anti-cancer activity [ 10 , 13 , 23 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. Cancer diseases are the main cause of death in the population, and many of their forms are characterized by high malignancy, metastasis, and resistance to chemotherapy [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition and Antimicrobial Properties of Honey Bee Venom

Isidorov¹,

Zalewski

Zambrowski

et al. 2023

Molecules

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Due to its great medical and pharmaceutical importance, honey bee venom is considered to be well characterized both chemically and in terms of biomedical activity. However, this study shows that our knowledge of the composition and antimicrobial properties of Apis mellifera venom is incomplete. In this work, the composition of volatile and extractive components of dry and fresh bee venom (BV) was determined by GC-MS, as well as antimicrobial activity against seven types of pathogenic microorganisms. One-hundred and forty-nine organic C1–C19 compounds of different classes were found in the volatile secretions of the studied BV samples. One-hundred and fifty-two organic C2–C36 compounds were registered in ether extracts, and 201 compounds were identified in methanol extracts. More than half of these compounds are new to BV. In microbiological tests involving four species of pathogenic Gram-positive and two species of Gram-negative bacteria, as well as one species of pathogenic fungi, the values of the minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) were determined for samples of dry BV, as well as ether and methanol extracts from it. Gram-positive bacteria show the greatest sensitivity to the action of all tested drugs. The minimum MIC values for Gram-positive bacteria in the range of 0.12–7.63 ng mL−1 were recorded for whole BV, while for the methanol extract they were 0.49–125 ng mL−1. The ether extracts had a weaker effect on the tested bacteria (MIC values 31.25–500 ng mL−1). Interestingly, Escherichia coli was more sensitive (MIC 7.63–500 ng mL−1) to the action of bee venom compared to Pseudomonas aeruginosa (MIC ≥ 500 ng mL−1). The results of the tests carried out indicate that the antimicrobial effect of BV is associated with the presence of not only peptides, such as melittin, but also low molecular weight metabolites.

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“…As drug resistance spreads worldwide, antibiotics are becoming less effective, leading to increased challenges in treating infections and higher mortality rates. Urgent development of new antimicrobial agents is necessary to combat resistant Gram-negative bacterial infections [1][2][3][4]. Over the past decade, nanotechnology has made significant advancements and is undergoing rapid development as a multidisciplinary scientific field.…”

Section: Introductionmentioning

confidence: 99%

Chitosan-silver nanoparticles conjugate mediated by endophytic fungus Talaromyces sp.7S1: antimicrobial, antibiofilm and anticancer

Hamed,

Emara,

Shawky

et al. 2023

Egypt. J. Chem.

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Antibiotic resistance has substantially risen worldwide. Considering this worrying condition, many studies on biogenic nanoparticles and their antimicrobial and anticancer properties have been conducted. There are numerous ways to improve stability and dispersion through conjugate peroration with bioactive natural ingredients such as chitosan. Here, a fungal isolate was isolated and identified as Talaromyces sp. 7S1 by 18SrRNA with accession no. OP295112. This strain prepared biogenic silver nanoparticles (AgNPs) and chitosan-AgNPs conjugate (Ch-AgNPs). Various imaging and analytical methods including UV-vis, XRD, FTIR-spectroscopy, TEM, and SEM were utilized to characterize biogenic nanoparticles and conjugates. The biosynthesized AgNPs along with the prepared conjugate were evaluated for their antimicrobial effects on Gram-positive (Staphylococcus aureus (RCMB 010028) and Bacillus subtilis (RCMB 010067)) and Gram-negative (Escherichia coli )RCMB 010052) and Salmonella typhi)RCMB 010052)) pathogens. Ch-AgNPs showed good antimicrobial activity compared to control. Results revealed that the crude extract showed antibacterial activity against E. coli and S. aureus while a substantial effect was observed with AgNPs. AgNPs displayed potent antibacterial activity against E. coli, S. aureus, and B. subtilis, while they showed moderate activity against S. typhi. Furthermore, Ch-AgNPs exhibited a pronounced antibacterial activity against E. coli, and S. aureus and low antibacterial activity against S. typhi. On the other hand, the obtained AgNPs and Ch-AgNPs exhibited a pronounced antibiofilm activity against all tested strains. Additionally, the antioxidant and anticancer activities of AgNPs and Ch-AgNPs were also evaluated. Collectively, AgNPs and Ch-AgNPs exhibit good antimicrobial activity, nevertheless, Ch-AgNPs conjugate reduced the toxicity of AgNPs which may represent a safer tool to inhibit bacterial pathogens.

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Characterization, antimicrobial and antitumor activity of superoxide dismutase extracted from Egyptian honeybee venom (Apis mellifera lamarckii)

Cited by 5 publications

References 58 publications

Bio-production and characterization of carotenoid yellow pigment from Kocuria sp. GMA and exploring its sustainable antioxidant, antimicrobial and antibiofilm properties

Bio-production and characterization of carotenoid yellow pigment from Kocuria sp. GMA and exploring its sustainable antioxidant, antimicrobial and antibiofilm properties

Chemical Composition and Antimicrobial Properties of Honey Bee Venom

Chitosan-silver nanoparticles conjugate mediated by endophytic fungus Talaromyces sp.7S1: antimicrobial, antibiofilm and anticancer

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