Characterization, antioxidant and α-amylase inhibition of polysaccharides and phosphorylated derivatives from finger citron (Citrus medica L. var. sarcodactylis Swingle)

Wang, Feilin; Hu, Huizhong; Li, Yufu; Zhong, Jinhong; Pan, Haihui; Sheng, Yuanhao; Bi, Yongguang; Kong, Fansheng

doi:10.1016/j.fbio.2024.103985

Cited by 2 publications

(1 citation statement)

References 54 publications

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“…This solution was cooled and diluted to 10.0 mL, and the absorbance was measured at 540 nm with the PBS used as a control group. The results were graphed, and the optimum amount of enzyme for the reaction was calculated (Wang et al, 2024).…”

Section: Determination Of the Optimum Enzyme Concentration And Reacti...mentioning

confidence: 99%

Structural characterization and hypoglycemic effect of polysaccharides of Polygonatum sibiricum

Wang,

Yang,

Shen

et al. 2024

Journal of Food Science

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Polygonatum sibiricum polysaccharide (PSP) was extracted and purified from raw material obtained from P. sibiricum. The structural features of PSP were investigated by Congo red, circular dichroism spectrum, high‐performance gel permeation chromatography, scanning electron microscope, atomic force microscope, ultraviolet spectroscopy, and Fourier transform infrared spectroscopy analysis. In vitro simulations were conducted to investigate the kinetics of PSP enzyme inhibition. Moreover, a type II diabetes mouse model (T2DM) with streptozotocin‐induced insulin resistance was established, and the indexes of lipid quadruple, insulin resistance index, oral glucose tolerance (OGTT), organ index, and pancreatic morphology of model mice were measured. The results showed that PSP mainly consists of monosaccharides, such as mannose, glucose, galactose, xylose, and arabinose. It also has a β‐glycosidic bond of a pyranose ring and an irregular reticulated aggregated structure with a triple helix. In vitro enzyme inhibition assays revealed that PSP acts as a reversible competitive inhibitor of α‐glucosidase and α‐amylase. Furthermore, PSP was found to reduce insulin resistance index, increase OGTT and serum insulin levels, decrease free fatty acid content to improve lipid metabolism, and lower glycated serum protein content to enhance glucose metabolism in T2DM mice, thereby leading to a reduction in blood glucose concentration. Additionally, PSP exhibited reparative effects on the damaged liver tissue cells and pancreatic tissue in T2DM mice. The experiment results provide a preliminary basis for the therapeutic mechanism of PSP about type II diabetes and a theoretical reference for application in food and pharmaceutical development.

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Section: Determination Of the Optimum Enzyme Concentration And Reacti...mentioning

confidence: 99%

Structural characterization and hypoglycemic effect of polysaccharides of Polygonatum sibiricum

Wang,

Yang,

Shen

et al. 2024

Journal of Food Science

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Fabrication and Characterization of Gelatin-Finger Citron Polysaccharide Nanoparticles for Enhanced Solubility and Bioavailability of Luteolin in Treating Acute Alcoholic Liver Disease

Wang,

Liu,

Cai

et al. 2024

J. Agric. Food Chem.

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Luteolin (Lut) is a natural flavonoid that has been widely used in the treatment of liver and glycolipid metabolic diseases. However, poor water solubility and bioavailability limit its efficacy and application. To overcome these challenges, a protein−polysaccharide composite nanoparticle (L-GF NPs) for Lut delivery was prepared by a self-assembly method based on amphiphilic gelatin and active finger citron polysaccharide (FCP). The L-GF NPs were approximately spherical in shape and were formed by hydrogen bonding, electrostatic interaction, and hydrophobic interaction. Lut was encapsulated in the nanoparticles in an amorphous state, and the encapsulating efficiency was 79.49%. The efficacy of L-GF NPs was evaluated by intragastric administration of an alcoholic liver disease (ALD) mouse model. L-GF NPs have been demonstrated to significantly ameliorate the symptoms of ALD mice, which are mainly achieved by reducing lipid accumulation and oxidative stress and inhibiting the NF-κB pathway. In conclusion, the aqueous solubility and bioavailability of Lut were markedly enhanced by FCP encapsulation. Meanwhile, the combined effect of Lut and FCP significantly ameliorated ALD and attenuated alcohol-induced lipid metabolic disorders and oxidative damage. This provides a new strategy to promote the prevention and treatment of ALD.

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Characterization, antioxidant and α-amylase inhibition of polysaccharides and phosphorylated derivatives from finger citron (Citrus medica L. var. sarcodactylis Swingle)

Cited by 2 publications

References 54 publications

Structural characterization and hypoglycemic effect of polysaccharides of Polygonatum sibiricum

Structural characterization and hypoglycemic effect of polysaccharides of Polygonatum sibiricum

Fabrication and Characterization of Gelatin-Finger Citron Polysaccharide Nanoparticles for Enhanced Solubility and Bioavailability of Luteolin in Treating Acute Alcoholic Liver Disease

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