1993
DOI: 10.1111/1523-1747.ep12354098
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Characterization, Barrier Function, and Drug Metabolism of an In Vitro Skin Model

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Cited by 101 publications
(47 citation statements)
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“…The permeability of our reconstructed epi dermis was similar to that of other skin substi tutes f 18,19,[22][23][24][25][26][27], No correlation between structure, biochemistry and barrier function has been yet established. We consider that studies of reconstructed skin should focus on perfecting barrier function, a relatively simple parameter to measure, before turning to struc ture and biochemistry.…”
Section: Discussionmentioning
confidence: 52%
See 1 more Smart Citation
“…The permeability of our reconstructed epi dermis was similar to that of other skin substi tutes f 18,19,[22][23][24][25][26][27], No correlation between structure, biochemistry and barrier function has been yet established. We consider that studies of reconstructed skin should focus on perfecting barrier function, a relatively simple parameter to measure, before turning to struc ture and biochemistry.…”
Section: Discussionmentioning
confidence: 52%
“…However, like other models, it was respectively 10 and 5 times more permeable for water and estradiol than human native epidermis incubated overnight at 37°C in a humidified atmosphere [19,24], The increased permeability of reconstructed epidermis is to some extent due to an in creased hydration provoked by the medium through the membrane, as well as the humidi ty of atmosphere [23], Furthermore, air- Table 6. M ean fluxes a n d c u m u la tiv e percentages o f tritia te d w ater a n d estradiol p e n etratio n through n a tiv e a n d re co n stru c te In these experiments, reconstructed epidermis (RE) is grown in conditioned medium derived from proliféra-tive fibroblasts (CM) on noncoated membranes, or laminin (LM)-, fibronectin (FNK type I collagen (C 1F or type IV collagen (C4)-coated membranes.…”
Section: Discussionmentioning
confidence: 74%
“…The success in the clinic has led scientists to believe that these models could be considered as a feasible alternative to replace the traditional skin models in toxicological and pharmacological studies in vitro. Recently these models have been used for shortterm cytotoxicity assays [7] and skin metabolism studies [8] . However, their use in vitro is still limited due to a deficient barrier function.…”
Section: Introductionmentioning
confidence: 99%
“…Although human skin models were shown to be more permeable than human skin ex vivo, they seemed to correctly predict the permeability rank order of compounds with different physicochemical properties when they were applied in simple vehicles at infinite doses [4][5][6][7]. However, only few studies have been reported on human skin models dealing with vehicle effects on percutaneous penetration [4,[8][9][10][11][12].…”
Section: Introductionmentioning
confidence: 99%