Characterization by liquid chromatography–mass spectrometry and antioxidant activity of an ethanolic extract of Inula viscosa leaves

Kheyar-Kraouche, Naoual; Bento-Silva, Andreia; Serra, Ana Teresa; Bedjou, Fatiha; Bronze, Maria Rosário

doi:10.1016/j.jpba.2018.04.047

Cited by 45 publications

(47 citation statements)

References 63 publications

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“…Used as a medicinal plant in different countries, I. viscosa has been characterized for its complex chemical composition, in order to identify bioactive compounds responsible for its biological activities. 46,47 Recently, Kheyar-Kraouche et al 48 analyzed an ethanolic extract from I. viscosa leaves growing in Algeria using high-performance liquid chromatography (HPLC) coupled to photodiode array detection and electrospray ionization mass spectrometry methodologies, and observed 51 phytochemical compounds including phenolic acids, flavonoids, lignans, and terpenoids, 26 of which were identified for the first time and belong to different families of compounds. Different biologically active compounds, belonging to several families, isolated from I. viscosa were tested alone or in association on cancer cell lines [49][50][51][52] or in ''in vivo'' 53,54 experiments showing strong antineoplastic activity.…”

Section: Discussionmentioning

confidence: 99%

Antiproliferative and proapoptotic effects of Inula viscosa extract on Burkitt lymphoma cell line

et al. 2020

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Burkitt lymphoma is a very aggressive B-cell non-Hodgkin lymphoma. Although remarkable progress has been made in the therapeutic scenario for patients with Burkitt lymphoma, search and development of new effective anticancer agents to improve patient outcome and minimize toxicity has become an urgent issue. In this study, the antitumoral activity of Inula viscosa, a traditional herb obtained from plants collected on the Asinara Island, Italy, was evaluated in order to explore potential antineoplastic effects of its metabolites on Burkitt lymphoma. Raji human cell line was treated with increasing Inula viscosa extract concentration for cytotoxicity screening and subsequent establishment of cell cycle arrest and apoptosis. Moreover, gene expression profiles were performed to identify molecular mechanisms involved in the anticancer activities of this medical plant. The Inula viscosa extract exhibited powerful antiproliferative and cytotoxic activities on Raji cell line, showing a dose- and time-dependent decrease in cell viability, obtained by cell cycle arrest in the G2/M phase and an increase in cell apoptosis. The treatment with Inula viscosa caused downregulation of genes involved in cell cycle and proliferation (c-MYC, CCND1) and inhibition of cell apoptosis (BCL2, BCL2L1, BCL11A). The Inula viscosa extract causes strong anticancer effects on Burkitt lymphoma cell line. The molecular mechanisms underlying such antineoplastic activity are based on targeting and downregulation of genes involved in cell cycle and apoptosis. Our data suggest that Inula viscosa natural metabolites should be further exploited as potential antineoplastic agents against Burkitt lymphoma.

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Section: Discussionmentioning

confidence: 99%

Antiproliferative and proapoptotic effects of Inula viscosa extract on Burkitt lymphoma cell line

et al. 2020

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“…For example, a recent study revealed the presence of 51 compounds, including 11 phenolic acids, 23 flavonoids, one lignin, and 12 terpenoids in ethanol extract of IV leaves. Twenty six of these compounds were described for the first time in IV (20). Several studies have shown the anti-proliferative activity of IV extracted from different parts of the plant and by different solvents.…”

Section: Discussionmentioning

confidence: 99%

Inula Viscosa Extract Inhibits Growth of Colorectal Cancer Cells in vitro and in vivo Through Induction of Apoptosis

et al. 2019

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Colorectal cancer (CRC) is the second most common cancer in females and the third in males worldwide. Conventional therapy of CRC is limited by severe side effects and by the development of resistance. Therefore, additional therapies are needed in order to combat the problem of selectivity and drug resistance in CRC patients. Inula viscosa (IV) is a well-known medicinal perennial herb in traditional medicine. It is used for different therapeutic purposes, such as; topical anti-inflammatic, diuretic, hemostatic, antiseptic, antiphlogistic, and in the treatment of diabetes. Several studies attempted to reveal the anti-cancer activity of different extracts prepared by different organic solvents from different parts of the IV plant. The aim of the present study is to examine the potential beneficial effects of IV leaf aqueous extract on the growth of colon cancer cells in vitro and in vivo . The results indicated that exposure of colorectal cancer cells to IV extract, significantly reduced cell viability in a dose and time dependent manner. Moreover, treatment of cells with 300 μg/ml of IV extract induced apoptosis, as it was detected by Annexin V/FITC/PI, TUNEL assay, and the activation of caspases. In vivo studies revealed that treatment with 150 or 300 mg/kg IV extract inhibited tumor growth in mice transplanted with MC38 cells. Tumors' weight and volume were significantly ( P < 0.001) reduced when compared to untreated-control group. Staining of the paraffin section of tumors revealed that IV treatment inhibited cell proliferation and induced apoptosis. Additionally, no side effects such as; weight loss, behavior changes, ruffled fur or changes in kidney, and liver functions were observed. These results may indicate that active doses of IV extract are not toxic. Further studies are needed in order to identify the structure of the active compounds. Results from this study may contribute to the development of new and efficient strategies for treatment of human colon cancer.

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“…Plants belonging to this genus have shown to possess various biological activities–anti‐inflammatory, antitumor, antibacterial, antitussive, antiproliferative, antioxidant, antidiabetic and hepatoprotective, etc., which were attributed to the abundance of bioactive components mainly sesquiterpene lactones, phenolic acids, and flavonoids . Chlorogenic acid and dicaffeoyl esters of quinic acid have been also reported in some Inula species …”

Section: Introductionmentioning

confidence: 99%

Caffeoylquinic Acids, Cytotoxic, Antioxidant, Acetylcholinesterase and Tyrosinase Enzyme Inhibitory Activities of Six Inula Species from Bulgaria

Трендафилова

Ivanova

Rangelov

et al. 2020

Chemistry & Biodiversity

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Chlorogenic (5-CQA), 1,5-, 3,5-, 4,5-and 3,4-dicaffeoylquinic (DCQA) acids were identified and quantified in the methanol extracts of Inula oculus-christi L., I. bifrons L., I. aschersoniana JANKA var. aschersoniana, I. ensifolia L., I. conyza (GRIESS.) DC. and I. germanica L. by HPLC analysis. The amount of 5-CQA varied from 5.48 to 28.44 mg/g DE and the highest content was detected in I. ensifolia. 1,5-DCQA (4.05-55.25 mg/g DE) was the most abundant dicaffeoyl ester of quinic acid followed by 3,5-DCQA, 4,5-DCQA and 3,4-DCQA. The extract of I. ensifolia showed the highest total phenolic content (119.92 � 0.95 mg GAE/g DE) and exhibited the strongest DPPH radical scavenging activity (69.41 � 0.55 %). I. bifrons extract was found to be the most active sample against ABTS * + (TEAC 0.257 � 0.012 mg/mL) and the best tyrosinase inhibitor. The studied extracts demonstrated a low inhibitory effect towards acetylcholinesterase and possessed low cytotoxicity in concentration range from 10 to 300 μg/mL toward non-cancer (MDCK II) and cancer (A 549) cells. Figure 2. HPLC chromatograms of the studied extracts and standard mixture.[a] Results are presented as mean � SD from three replicates (n = 3). Means in the columns with the same letter are not significantly different from each other (p > 0.05) (t-test).

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Characterization by liquid chromatography–mass spectrometry and antioxidant activity of an ethanolic extract of Inula viscosa leaves

Cited by 45 publications

References 63 publications

Antiproliferative and proapoptotic effects of Inula viscosa extract on Burkitt lymphoma cell line

Antiproliferative and proapoptotic effects of Inula viscosa extract on Burkitt lymphoma cell line

Inula Viscosa Extract Inhibits Growth of Colorectal Cancer Cells in vitro and in vivo Through Induction of Apoptosis

Caffeoylquinic Acids, Cytotoxic, Antioxidant, Acetylcholinesterase and Tyrosinase Enzyme Inhibitory Activities of Six Inula Species from Bulgaria

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