Characterization, enzymatic activity and biofilm formation of Candida species isolated from goat milk

Zaragoza, Carolina Segundo; Ortiz, Itzel López; Castillo, David Alejandro Contreras Caro del; Hernández, Yesmín María Domínguez; García, Juan Antonio Rodríguez

doi:10.1016/j.riam.2021.06.001

Cited by 4 publications

(4 citation statements)

References 29 publications

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“…Şeker and Özenç (2011) in Turkey in 2011 conducted a study on 8 C. kefyr isolates, finding that 62.5% of isolates could produce biofilms. In another study, Zaragoza et al (2021) reported in 2021 that 73.4% of C. kefyr isolates could produce biofilms. In our study, the presence of biofilm formation among 81.8% of the isolates highlights the potential for this species to colonise and adhere to surfaces, contributing to its pathogenicity and resistance to antifungal treatment.…”

Section: Discussionmentioning

confidence: 96%

“…Various studies have identified Candida species, including kefyr , residing in dairy products like milk and cheese. Furthermore, C. kefyr has been identified as a source of mastitis in ruminant animals like cows and goats, which impacts both the milk's quality and quantity, and it has also been detected in the milk of asymptomatic goats (Zaragoza et al, 2021 ). The consumption of dairy products that contain C. kefyr may facilitate the transmission of these organisms to individuals who are at increased risk, particularly hospitalised patients and those with underlying medical conditions (Nurdin et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of enzymatic profiles and biofilm formation in clinical and environmental Candida kefyr isolates

Nouraei,

Zare,

Nemati

et al. 2024

Environ Microbiol Rep

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The global landscape of Candida infections has seen a significant shift. Previously, Candida albicans was the predominant species. However, there has been an emergence of non‐albicans Candida species, which are often less susceptible to antifungal treatment. Candida kefyr, in particular, has been increasingly associated with infections. This study aimed to investigate the profiles of enzymatic activity and biofilm formation in both clinical and non‐clinical isolates of C. kefyr. A total of 66 C. kefyr isolates were analysed. The activities of proteinase and phospholipase were assessed using bovine serum albumin and egg yolk agar, respectively. Haemolysin, caseinolytic and esterase activities were evaluated using specific methods. Biofilm formation was investigated using crystal violet staining. The findings indicated that biofilm and proteinase activity were detected in 81.8% and 93.9% of all the isolates, respectively. Haemolysin activity was observed with the highest occurrence (95.5%) among normal microbiota isolates. Esterase activity was predominantly identified in dairy samples and was absent in hospital samples. Caseinase production was found with the highest occurrence (18.2%) in normal microbiota and hospital samples. Phospholipase activity was limited, found in only 3% of all the isolates. These findings reveal variations in enzyme activity between clinical and non‐clinical C. kefyr isolates. This sheds light on their pathogenic potential and has implications for therapeutic strategies.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Comparative analysis of enzymatic profiles and biofilm formation in clinical and environmental Candida kefyr isolates

Nouraei,

Zare,

Nemati

et al. 2024

Environ Microbiol Rep

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“…parapsilosis isolates were subjected to susceptibility testing by standard methods for the antifungal test used in this study [32,33]. A standard reference procedure has been described by the National Committee for Clinical Laboratory Standards [34]. Antifungal susceptibility was determined by using five antifungal discs (Amphotericine B, Fluconazole, Ketoconazole, Nystatine, and Itraconazole) according to guidelines recommended by the [32], corresponding to the drugs considered routine testing and reporting on yeast.…”

Section: Susceptibility For Some Antifungal Drugsmentioning

confidence: 99%

Molecular Detection of Virulence Factor Genes in Candida parapsilosis Isolated from Subclinical Mastitis Goats and Antifungal Susceptibility in Mosul Province

Al-abedi,

Khalil

2025

Egyptian Journal of Veterinary Sciences

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HIS STUDY aimed to isolate and diagnose Candida parapsilosis. The utilization of molecular technologies for identification and virulence gene detection leads to the discovery of Subclinical Mastitis in goats. According to the California Mastitis Take, fifty goat milk samples with subclinical mastitis appearances were collected from specific areas of Mosul province. They were examined using rapid diagnostic tests, which were confirmed by a polymerase chain reaction (PCR) assay. Candida parapasilosis was identified in 20/50 (40%) of the mastitis milk samples. In relation to molecular techniques and the consequences of PCR amplification of the 18S rRNA gene for identification of C. parapasilosis, the gene was present in 20 samples. Moreover, the Metallo-aminopeptidase gene was present in 20/20 (100%), and the alkaline phosphatase sequence gene was present in 15/20 (75%). However, alpha-glucosidase was present in 10/20 (50%) and sterol esterase genes in 8/20 (40%). Consequence, Antifungal sensitivity testing results showed that fluconazole and amphotericine B provided the most exceptional senitivity against C. parapasilosis isolates, followed by ketoconazole, itraconazole, and nystatin.The outcome of the experiment indicates that goat milk contains many species of the Candida genus, with a significant prevalence of C. parapasilosis. This particular species may act as an opportunistic pathogen in mastitis.

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“…Segundo Zaragoza et al. ( 2021 ) found that 24% of 221 ‘ Candida ’ isolates from the milk of healthy goats were D. hansenii . However, the isolates had not caused disease and the species identification is uncertain.…”

Section: Assessmentmentioning

confidence: 99%

Update of the list of qualified presumption of safety (QPS) recommended microbiological agents intentionally added to food or feed as notified to EFSA 17: suitability of taxonomic units notified to EFSA until September 2022

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2023

EFS2

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The qualified presumption of safety (QPS) approach was developed to provide a regularly updated generic pre‐evaluation of the safety of microorganisms, intended for use in the food or feed chains, to support the work of EFSA's Scientific Panels. The QPS approach is based on an assessment of published data for each agent, with respect to its taxonomic identity, the body of relevant knowledge and safety concerns. Safety concerns identified for a taxonomic unit (TU) are, where possible, confirmed at the species/strain or product level and reflected by ‘qualifications’. In the period covered by this Statement, new information was found leading to the withdrawal of the qualification ‘absence of aminoglycoside production ability’ for Bacillus velezensis. The qualification for Bacillus paralicheniformis was changed to ‘absence of bacitracin production ability’. For the other TUs, no new information was found that would change the status of previously recommended QPS TUs. Of 52 microorganisms notified to EFSA between April and September 2022 (inclusive), 48 were not evaluated because: 7 were filamentous fungi, 3 were Enterococcus faecium , 2 were Escherichia coli , 1 was Streptomyces spp., and 35 were taxonomic units (TUs) that already have a QPS status. The other four TUs notified within this period, and one notified previously as a different species, which was recently reclassified, were evaluated for the first time for a possible QPS status: Xanthobacter spp. could not be assessed because it was not identified to the species level; Geobacillus thermodenitrificans is recommended for QPS status with the qualification ‘absence of toxigenic activity’. Streptoccus oralis is not recommended for QPS status. Ogataea polymorpha is proposed for QPS status with the qualification ‘for production purposes only’. Lactiplantibacillus argentoratensis (new species) is included in the QPS list.

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Characterization, enzymatic activity and biofilm formation of Candida species isolated from goat milk

Cited by 4 publications

References 29 publications

Comparative analysis of enzymatic profiles and biofilm formation in clinical and environmental Candida kefyr isolates

Comparative analysis of enzymatic profiles and biofilm formation in clinical and environmental Candida kefyr isolates

Molecular Detection of Virulence Factor Genes in Candida parapsilosis Isolated from Subclinical Mastitis Goats and Antifungal Susceptibility in Mosul Province

Update of the list of qualified presumption of safety (QPS) recommended microbiological agents intentionally added to food or feed as notified to EFSA 17: suitability of taxonomic units notified to EFSA until September 2022

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