Characterization, expression, and functional analysis of testis-specific serine/threonine kinase 1 (<i>Tssk1</i>) in the pen shell<i>Atrina pectinata</i>

Li, Haohao; Kong, Lingfeng; Yu, Ruihai; Yu, Hong; Li, Qi

doi:10.1080/07924259.2016.1161667

Cited by 21 publications

(20 citation statements)

References 30 publications

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“…This finding supports our explanation that the abalone TSSK1-like gene plays a role in the late or final phase of male germ cell differentiation, which is essentially congruent with largely accumulated evidence with mammalian TSSK1s [4,5]. Data from this study are also well in agreement with previous observation of the TSSK1-like homolog in Atrina pectinata (pen shell) [10] and the TSSK2-like homolog in Agropecten purpuratus (scallop) [44]. However, there is a discordance between present findings and previous observation of TSSK1-like (partial suppression subtraction hybridization clone) in Mytilus edulis (mussel), in which the mussel TSSK1-like homolog was upregulated in early developing testis relative to mature testis [9].…”

Section: Discussionsupporting

confidence: 93%

“…Molecular phylogeny-based classification analysis suggests that abalone TSSK1-like cloned in this study has a phylogenetic affiliation to human TSSK1. Comparison of 3D structure between abalone TSSK1-like and human TSSK1 (TSSK1B) has also indicated that high similarity in overall shape and in several regions known to be essential in the structure and function of the TSSK1 protein, which is also broadly congruent with the 3D model predicted for the TSSK1-like protein from a bivalve species [10]. Taken together, cDNA and amino acid sequences suggest structural orthology of abalone TSSK1-like to human TSSK1.…”

Section: Discussionmentioning

confidence: 63%

“…At the genomic level, all molluscan sequences annotated as TSSK1 (or TSSK1-like) genes may not display the intronless gene organization, which is discordant with the single exon structure that has been invariantly found in most mammalian TSSK1 genes. More importantly, barring only a few previous reports in bivalves [9,10], few studies have characterized TSSK1 (and TSSK1-like) expression in molluscans, particularly in gastropodian organisms.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of testis-specific serine/threonine kinase 1-like (TSSK1-like) gene and expression patterns in diploid and triploid Pacific abalone (Haliotis discus hannai; Gastropoda; Mollusca) males

Kim

Park

et al. 2019

PLoS ONE

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Testis-specific serine/threonine kinase 1-like (TSSK1-like), which plays important roles in late-phase spermatogenesis and male fertility, was characterized in Pacific abalone Haliotis discus hannai, an important commercial marine gastropod. Further, its expression patterns were assessed in diploid and induced triploid males showing differential degrees of testis maturation. Abalone TSSK1-like shared conserved structural features with mammalian TSSK1s and other potential metazoan orthologs, especially regarding the catalytic STKc domain. Phylogenetically, abalone TSSK1-like displayed a genetic affiliation with its molluscan TSSK1-like orthologs and human TSSK1. Additionally, abalone TSSK1-like gene showed a tetrapartite exon-intron organization, unlike the intronless structure of most amniotic tetrapodian TSSK1s. Molecular phylogenetic analysis in the metazoan lineage suggested a possible revision in the origin of the earliest ancestral TSSK1. Further, abalone TSSK1-like showed testis-predominant expression, which was significantly influenced by both age and seasonal reproductive cycles. Comparative expression analyses between diploid and triploid abalone males suggested that robust TSSK1-like expression occurred primarily at the post-meiotic stage. Additionally, RT-PCR assay indicates that mature abalone sperms retain TSSK1-like transcripts after release. Taken together, this study provides useful insights for further studies to assess male reproduction and sterility and/or partial fertility of induced male triploidy in abalone species.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

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Characterization of testis-specific serine/threonine kinase 1-like (TSSK1-like) gene and expression patterns in diploid and triploid Pacific abalone (Haliotis discus hannai; Gastropoda; Mollusca) males

Kim

Park

et al. 2019

PLoS ONE

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“…In recent years, a family of testis-specific protein kinases, called as testis-specific serine/threonine kinases (Tssks), has attracted researcher’s interest due to their essential roles in spermatogenesis ( Wang et al, 2015a ; Li et al, 2016 ; Kim et al, 2019 ). Six Tssk subfamilies have been reported in mammals, including Tssk1 through Tssk6, which have the conserved serine/threonine protein kinases catalytic (S_TKc) domain ( Wang et al, 2015a ).…”

Section: Introductionmentioning

confidence: 99%

Expression of the Testis-Specific Serine/Threonine Kinases Suggests Their Role in Spermiogenesis of Bay Scallop Argopecten irradians

Xue

Zhang

et al. 2021

Front. Physiol.

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Members of the testis-specific serine/threonine kinases (Tssk) family play critical roles in spermatogenesis in vertebrates. But in mollusks, research on Tssk family is still lagging. In this study, we systematically identified Tssk family based on the genomic and transcriptomic data from a commercially important scallop Argopecten irradians and detected the spatiotemporal expression in adult gonads. Five members were identified, with the gene length varying from 1,068 to 10,729 bp and the protein length ranging from 294 to 731 aa. All the Tssks possess a serine/threonine protein kinase catalytic (S_TKc) domain. Phylogenetic analysis revealed existence of four homologs of vertebrate Tssk1/2, Tssk3, Tssk4, Tssk5, and absence of Tssk6 in the scallop. The remaining gene (Tssk7) formed an independent clade with Tssks of other mollusks and arthropods, indicating that it may be a new member of Tssk family unique to protostomes. By investigating the expression of Tssks in four developmental stages of testes and ovaries, we found all five Tssks were primarily expressed in mature testis. In situ hybridization experiment revealed the five Tssks were localized in the spermatids and spermatozoa. The testis-predominant expression of Tssk family suggests Tssks may play pivotal roles in spermiogenesis in the scallop. Our study provides basic information on the characteristics and expression profiles of Tssk family of A. irradians. To our knowledge, it represents the first comprehensive analysis of Tssk family in mollusks.

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“…Until now, five members of the TSSK family have been recognized, and all are significantly expressed in the testis [15]. Different members of the TSSK gene family have been known to be expressed at various stages of spermatogenesis [16,17,18,19]. Tssk1 was identified as the first member of the TSSKs family [20].…”

Section: Introductionmentioning

confidence: 99%

RNAi-Mediated Knockdown of Tssk1 and Tektin1 Genes Impair Male Fertility in Bactrocera dorsalis

Sohail

Tariq

Zheng

et al. 2019

Insects

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The genetic-based sterile insect technique (SIT) is an effective and environmentally safe strategy to diminish populations of agricultural and horticultural insect pests. Functional characterization of genes related to male fertility can enhance the genetic-based SIT. Tssk1 has been involved to control male fertility in both mammals and insects. Moreover, Tektin1 has also been revealed to influence male fertility in both human and mammals. These findings suggested that Tssk1 and Tektin1 identified from Bactrocera dorsalis could be required for male fertility in B. dorsalis. In this study, expression profiles of these two genes were studied at different developmental stages and in various tissues of adult males. Remarkably, it was found that Tssk1 and Tektin1 were highly expressed in the testis of mature adult males of B. dorsalis. Furthermore, Tssk1 and Tektin1 genes were downregulated by using the RNA interference (RNAi) method. Fertility assays including egg laying, hatching, and spermatozoa count were also performed to investigate male fertility of B. dorsalis. Results showed that knockdown of Tssk1 and Tektin1 caused male sterility up to 58.99% and 64.49%, respectively. As expected, the total numbers of spermatozoa were also significantly reduced by 65.83% and 73.9%, respectively. These results suggested that male sterility was happened wing to the low number of spermatozoa. In conclusion, we demonstrate that Tssk1 and Tektin1 are the novel agents that could be used to enhance the genetic-based SIT, or their double-stranded RNA (dsRNA) can be used as biopesticides to control the population of B. dorsalis.

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Characterization, expression, and functional analysis of testis-specific serine/threonine kinase 1 (Tssk1) in the pen shellAtrina pectinata

Cited by 21 publications

References 30 publications

Characterization of testis-specific serine/threonine kinase 1-like (TSSK1-like) gene and expression patterns in diploid and triploid Pacific abalone (Haliotis discus hannai; Gastropoda; Mollusca) males

Characterization of testis-specific serine/threonine kinase 1-like (TSSK1-like) gene and expression patterns in diploid and triploid Pacific abalone (Haliotis discus hannai; Gastropoda; Mollusca) males

Expression of the Testis-Specific Serine/Threonine Kinases Suggests Their Role in Spermiogenesis of Bay Scallop Argopecten irradians

RNAi-Mediated Knockdown of Tssk1 and Tektin1 Genes Impair Male Fertility in Bactrocera dorsalis

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