Characterization, gene cloning, and heterologous expression of β-mannanase from a thermophilic Bacillus subtilis

Summpunn, Pijug; Chaijan, Suttidarak; Isarangkul, Duangnate; Wiyakrutta, Suthep; Meevootisom, Vithaya

doi:10.1007/s12275-011-0357-1

Cited by 37 publications

(24 citation statements)

References 36 publications

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“…Mannanases also help in removal of lignin from pulps by biobleaching without affecting the quality of fi bers. Here are so many thermophilic microorganisms like Thermomyces lanuginosus , Malbranchea cinnamomea , Myceliophthora fergusii , and Bacillus subtilis which are able to produce thermostable mannanases (Maijala et al 2012 ;Summpunn et al 2011 ). The thermostable β-mannanases and β-mannosidases offer a signifi cant advantage for biobleaching at elevated temperatures.…”

Section: Biobleachingmentioning

confidence: 99%

Microbial Mannanases: Properties and Applications

Soni

Kango

2013

Advances in Enzyme Biotechnology

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Mannans are a major constituent of the hemicellulose fraction of lignocelluloses. Mannans perform distinct functions as structural components in cell walls of softwoods and storage functions in seeds. Enzymatic hydrolysis of mannan involves the backbone hydrolyzing endo-β-mannanases and β-mannosidases. Mannans are heteropolymeric and their hydrolysis also requires the action of β-glucosidases and side-chain cleaving α-galactosidases and acetyl mannan esterases. Microorganisms are therefore explored for the production of such repertoire of enzymes so that effective mannan hydrolysis can be achieved. The present chapter discusses the occurrence and structural properties of mannans in plant materials and its hydrolysis using enzymes sourced from various fungi and other microorganisms. The production and properties of mannanolytic enzymes, their cloning and expression in heterologous hosts, and their application have also been discussed .

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Section: Biobleachingmentioning

confidence: 99%

Microbial Mannanases: Properties and Applications

Soni

Kango

2013

Advances in Enzyme Biotechnology

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“…3). 이로 보아 분리균 YB-1402 BCC41051의 mannanase는 pH 5-11.5에서 안정하여 알칼리 조 건에서 안정성이 우수하며 (Summpunn et al, 2011), 곰팡이인 Thielavia arenaria XZ7의 효소는 YB-1402와 유사하게 pH 3-10 범위에서 안정하다고 보고되었다 (Lu et al, 2013).…”

Section: 효소 반응특성unclassified

Production and Properties of Mannanase by a Bacillus amyloliquefaciens Isolate

Yoon¹

2014

The Korean Journal of Microbiology

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In the acidic LB plate, a bacterial strain was isolated from homemade soybean paste as a producer of the extracellular mannanase. The isolate YB-1402, which was a Gram-positive rod-shaped bacterium with spore, has been identified as Bacillus amyloliquefaciens on the basis of its 16S rDNA sequence and biochemical properties. Maximum mannanase productivity of the isolate YB-1402 was reached approximately 150 U/ml in LB broth supplemented with konjac (3.0%). The molecular mass of YB-1402 mannanase was estimated to approximately 38.0 kDa by zymogram of the culture filtrate on SDS-PAGE. The mannanase of culture filtrate was the most active at 55℃ and pH 5.5. The mannanase activity was completely maintained after pre-incubation at pH 3.0 to 10.0 for 1 h. The predominant products resulting from the mannanase hydrolysis were mannose, mannobiose and mannotriose for LBG or mannooligosaccharides. The enzyme could hydrolyze mannooligosaccharides larger than mannobiose.Keywords: Bacillus amyloliquefaciens, mannanase, productivity, property *For correspondence. E-mail: ykh@wsu.ac.kr; Tel.: +82-42-630-9742; Fax: +82-42-636-2676 Mannan 다당류에 속하는 glucomannan, galactomannan과 galactoglucomannan은 mannose 잔기끼리 β-1,4 결합을 하거나 mannose와 glucose간에 β-1,4 결합을 한 형태의 기본 골격을 가 지며 acetyl이나 galactose 잔기가 mannose 잔기와 측쇄결합을 하고 있다. 이들과 같은 이형다당류는 식물 세포벽의 hemicelluloses, 식물의 종자와 덩이줄기에 존재하며, mannose 잔기간 결합으로 만 구성된 동형 다당류인 mannan은 상아야자 열매에 존재한다.

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“…Genes encoding -mannanase from Bacillus sp. have been cloned and expressed in heterologous expression systems, and properties of the recombinat -mannanase have been characterized (Yanhe et al, 2004;Summpunn et al, 2011;Liu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Cloning of Partial β-Mannanase Gene from Indonesia Marine Bacteria Bacillus Subtilis LBF-005

Yopi¹,

Rahmani²,

Amaniyah³

et al. 2017

Squalen Bull. Marine Fisheries Postharvest Biotech.

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The strain LBF-005 from marine bacteria have been already isolated and screened for mannanase degrading enzyme in submerged fermentation process. This strain was further identified by using 16S rRNA identification and showed that bacterium is belong to Bacillus subtilis that could produce mannanase with activity around 9.5 U/mL. The optimum pH and temperature for the activity of crude enzyme for mannanase were 6.0 and 50 o C, respectively. Cloning of mannanase gene from B. subtilis was conducted using six primer / set designed based on the homology analysis conserved region of several mannanases from bacteria (Bacillus sp.) glycosyl hydrolase (GH) family 26. Optimization of PCR conditions was performed by gradient PCR to obtain PCR product of -mannanase gene. The PCR product was obtained by the third primer combination and was estimated to be around 972-bp. Analysis of the nucleotide sequence indicated that the sequence has similarity with mananase gene from other Bacillus sp., such as the B. subtilis strain W LY-12, B. subtilis strain W L-8, B. subtilis strain CICC 10260, B.subtilis strain CD-25, B.subtilis strain G1, and Bacillus sp. SW U60 about 98%, 98%, 98%, 98%, 97% and 95%, respectively.

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Characterization, gene cloning, and heterologous expression of β-mannanase from a thermophilic Bacillus subtilis

Cited by 37 publications

References 36 publications

Microbial Mannanases: Properties and Applications

Microbial Mannanases: Properties and Applications

Production and Properties of Mannanase by a Bacillus amyloliquefaciens Isolate

Cloning of Partial β-Mannanase Gene from Indonesia Marine Bacteria Bacillus Subtilis LBF-005

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