Characterization, morphology and composition of biofilm and precipitates from a sulphate-reducing fixed-bed reactor

Stability investigations of zinc and cobalt precipitates immobilized by in situ bioprecipitation (ISBP) process

“…This indicates that the inactivation of bacterial cells by metal sulphide precipitates was overcome by the regeneration capacity of the bacterial population. This phenomenon was also reported by Remoundaki et al [35] in the biofilm of sulphate reducing fixed bed reactor. Fig.…”

Metal precipitation in an ethanol-fed, fixed-bed sulphate-reducing bioreactor

“…This bacterial community is less diverse than the community originating from the lactate-fed reactor ( Fig. 3(b), [34]) which was used as the inoculum for the ethanol-fed reactor presented in this paper. This result shows that the use of ethanol as carbon/electron source limited the growth of certain species present in the inoculum, maintaining a less diverse sulphatereducing community.…”

“…The reactor was inoculated by transferring sufficient support material with already grown bacterial biomass from a previously operated, lactate-fed bioreactor [33]. This mixed sulphate-reducing bacterial culture has already been characterized and described in detail [34].…”

“…A volume of 10 mL of each sample was vacuum filtered under sterile conditions through 0.22 m sterile membranes (Whatman) which were kept under aseptic conditions until treatment. The diversity and the sequencing of the bacterial community were examined as described in [34]. A dsrAB neighbor-joining tree was constructed from unambiguous amino acids on the calculated Kimura distances using the Jukes and Cantor method [38] implemented in the Treecon package [39].…”

Formation of Fe-sulfides in cultures of sulfate-reducing bacteria

“…Amorphous Fe-sulfide, or disordered mackinawite, is usually the initial precipitate that forms when dissolved iron is contacted with sulfide [15][16][17]. Rickard [18] reported that well crystallized mackinawite may take up to 2 years at 25 • C to form following initial precipitation.…”