Characterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross‐amplification in 10 other subgenera

Abstract

Premise of the study:Microsatellite primers were developed in the perennial herbs of the diploid-polyploid complex Veronica subsect. Pentasepalae (Plantaginaceae) to investigate the role that hybridization has played in the evolution of the group, which includes several endangered species.Methods and Results:Twelve pairs of primers leading to polymorphic and readable markers were identified and optimized from V. jacquinii and V. orbiculata using a microsatellite-enriched library method and 454 GS-FLX technique. The set of primers amplified dinucleotide to pentanucleotide repeats, and the number of alleles per locus ranged from one to six, one to 11, and one to nine for V. orsiniana, V. javalambrensis, and V. rosea, respectively. Transferability analyses were performed in 20 species corresponding to 10 different subgenera.Conclusions:These results indicate the utility of the newly developed microsatellites across Veronica subsect. Pentasepalae, which will help in the study of gene flow patterns and genetic structure.

Transcriptomic resources and marker validation for diploid and polyploid Veronica (Plantaginaceae) from New Zealand and Europe

“…Natural species that are not associated with economically important crop or other “model” species often lack genomic resources and are very limited regarding the availability of variable genetic markers. Furthermore, developing and establishing such markers using traditional methods (e.g., López-González et al, 2015 ) can be tedious and time-consuming, with more effort required for fewer microsatellites developed. (As an aside, we found eight of the 12 reported SSR loci from López-González et al [2015] in our transcript sequences, none of which met the quality criteria of our QDD pipeline for our New Zealand–focused sampled species.)…”

“…Furthermore, developing and establishing such markers using traditional methods (e.g., López-González et al, 2015 ) can be tedious and time-consuming, with more effort required for fewer microsatellites developed. (As an aside, we found eight of the 12 reported SSR loci from López-González et al [2015] in our transcript sequences, none of which met the quality criteria of our QDD pipeline for our New Zealand–focused sampled species.) There are nearly 4000 plant transcriptomes in the NCBI Sequence Read Archive ( http://www.ncbi.nlm.nih.gov/sra/ ) and 1000 Plants (1KP) project ( www.onekp.com ; Matasci et al, 2014 ) online resources ( Hodel et al, 2016 ).…”

Microsatellite marker development for the tetraploid Veronica aragonensis (Plantaginaceae) using next‐generation sequencing and high‐resolution melting analyses

“…Kern. ; López‐González et al., ). However, preliminary cross‐transferability tests performed for most of these loci resulted either in monomorphic patterns or unsuccessful amplifications in V. aragonensis (results not shown).…”