Characterization of 18 new microsatellite loci in Atlantic cod (<i>Gadus morhua</i> L.)

Skírnisdóttir, Sigurlaug; Pampoulie, Christophe; Hauksdóttir, Sigurbjörg; Schulte, Inga; Olafsson, Kristinn; Hreggviðsson, Guðmundur Ó.; Hjörleifsdóttir, Sigríður

doi:10.1111/j.1755-0998.2008.02327.x

Cited by 10 publications

(7 citation statements)

References 8 publications

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“…From this sample, 757 individuals were assigned to 40 families that are 40 females and 20 males. Genotyping was determined from the following 16 microsatellites: Gmo8, Gmo19, Gmo37 (Miller, Le & Beacham ), Tch11, Tch14 (O'Reilly, Canino, Bailey & Bentzen ), Gmo38 (Jakobsdottir, Jorundsdottir, Skirnisdottir, Hjorleifsdottir, Hreggvidsson, Danielsdottir & Pampoulie ), PGmo38, PGmo49, PGmo61‐FRb, PGmo71, PGmo74, PGmo87, PGmo94, PGmo100, PGmo124 and PGmo134 (Skirnisdottir, Pampoulie, Hauksdottir, Schulte, Olafsson, Hreggvidsson & Hjorleifsdottir ).…”

Section: Methodsmentioning

confidence: 99%

Strong phenotypic and genetic correlation between size and first maturity in Atlantic codGadus morhuaL. reared in commercial conditions

Kristjánsson

Árnason

2014

Aquac Res

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Three‐year classes of Atlantic cod Gadus morhua were studied throughout their rearing in the eastern coast of Iceland from 2004 to 2011. The growth and status of maturity were recorded during the rearing. For one of the year classes, genetic parameters for body weight and maturity status were estimated from 757 individuals, which were the offspring of 40 dams and 20 sires. The estimate for heritability of body weight was h2 = 0.34 at the average weight of 630 g, and heritability for proportion of maturity was h2 = 0.17 given the same weight. The relationship between body weight and the proportion of mature individuals at first winter revealed a strong genetic correlation of rG = 0.90. The phenotypic relationship between body weight and proportion of maturity was estimated with a Bayesian logistic regression as P(yi = 1(mature)) = Φ(β0 + β1weight + β2sex). The best fit yielded β0 = −2.9320 with a 95% interval between −3.2807 and −2.5394, β1weight = 0.0041 with a 95% interval ranging from 0.0035 to 0.0046 and β1sex = −0.0201 with a 95% interval from −0.2003 to 0.1445. The gender had no notable effect. This strong phenotypic and genetic correlation in body weight and maturation suggests that an increased growth rate will consequently lead to a higher proportion of mature individuals in the population. As a consequence, genetic manipulations to simultaneously increase growth and delay maturation may present a challenge.

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Section: Methodsmentioning

confidence: 99%

Strong phenotypic and genetic correlation between size and first maturity in Atlantic codGadus morhuaL. reared in commercial conditions

Kristjánsson

Árnason

2014

Aquac Res

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“…To attain the goal of 20 microsatellite loci, 19 walleye pollock loci (O'Reilly et al 2000), 19 Atlantic cod loci (Miller et al 2000;Stenvik et al 2006;Wesmajervi et al 2007;Skirinsdottir et al 2008), and 11 Pacific cod loci (Cunningham et al 2009) were screened. One additional locus (Gma107), which had not been used previously (Canino et al 2005), was also evaluated.…”

Section: Methodsmentioning

confidence: 99%

Landscape Genetics Reveals Population Subdivision in Bering Sea and Aleutian Islands Pacific Cod

Spies

2012

Trans Am Fish Soc

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Landscape genetics of Pacific cod Gadus macrocephalus within the Bering Sea and Aleutian Islands (BSAI) management area of Alaska was examined in samples from nine spawning locations, including one temporal replicate sample, using 17 microsatellite DNA markers. This study examined fine‐scale population structure of Pacific cod in the BSAI with the goal of identifying breaks in genetic continuity associated with physical barriers to migration and larval transport. Samples were taken from spawning fish collected from the western Aleutian Islands east to Unimak Pass and as far north as the Pribilof Islands. Overall, this work confirms previous studies, which found that genetic differentiation between samples is proportional to the distance between them, and also resolves population structure on a scale not previously possible. Results indicate that dispersal is not continuous at a fine scale and several distinct genetic groups were identified that correspond to differences in the physical environment of the BSAI. The data provide evidence for limited connectivity among spawning groups; in particular, there is evidence that a barrier exists between the Aleutian Islands and the eastern Bering Sea. In addition, analysis of molecular variance and Monmonier algorithm results suggest that, within the Bering Sea, the Unimak Pass and Pribilof Islands spawning groups may be distinct from each other, and the Wombling analysis indicates that samples west of Amchitka Pass in the western Aleutians may be distinct from those in the eastern Aleutians.

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“…Samples were genotyped at 18 polymorphic microsatellite loci: Gmo2 (Brooker et al 1994), Gmo8, Gmo19, Gmo34, Gmo37 (Miller et al 2000), Tch5, Tch11, Tch14, Tch22 (O'Reilly et al 2000, PGmo38, PGmo49 (Jakobsdóttir et al 2006), andPGmo61, PGmo74, PGmo87, PGmo94, PGmo100, PGmo124, andPGmo134 (Skirnisdottir et al 2008). Polymerase chain reaction (PCR) conditions followed the method of Pampoulie et al (2006) for the first set of markers (Gmo8,Gmo19,Gmo34,Gmo37,Tch5,Tch11,Tch14,and Tch22) and of Skirnisdottir et al (2008) for the second set (PGmo38,PGmo49,PGmo61,PGmo74,PGmo87,PGmo94,PGmo100,PGmo124,and PGmo134). Samples were then analyzed on an ABI PRISM 3730 sequencer with the GeneScan-500 LIZ size standard and genotyping was performed with GeneMapper version 3.5 (Applied Biosystems, Foster City, California).…”

Section: Methodsmentioning

confidence: 99%

Neutral and Nonneutral Genetic Markers Revealed the Presence of Inshore and Offshore Stock Components of Atlantic Cod in Greenland Waters

Pampoulie¹,

Daníelsdóttir²,

Storr-Paulsen³

et al. 2011

Trans Am Fish Soc

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The spawning stock of Atlantic cod Gadus morhua in West Greenland waters was characterized by a drastic decline in the late 1960s and has since exhibited considerable variation. It has been suggested that the cod stock in West Greenland waters is composed of several stock components that include (1) a number of distinct local inshore populations spawning in separate fjord systems, (2) an offshore spawning component located on the fishing banks, and (3) a periodic Icelandic–East Greenland cod influx that mixes with the offshore and inshore West Greenland stock components. In an attempt to clarify the status of Atlantic cod in Greenland waters, we investigated the genetic structure at different inshore and offshore feeding grounds east and west of Greenland. A total of 1,581 genetic samples were collected within North Atlantic Fisheries Organization areas at inshore and offshore locations as well as within the International Council for the Exploration of the Sea area XIVb. Those samples were genotyped for 18 microsatellite loci and the pantophysin (Pan I) locus. Both types of genetic markers gave congruent results and suggest the presence of two distinct genetic components with limited connectivity in Greenland waters, namely, an inshore component and an offshore component.

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Characterization of 18 new microsatellite loci in Atlantic cod (Gadus morhua L.)

Cited by 10 publications

References 8 publications

Strong phenotypic and genetic correlation between size and first maturity in Atlantic codGadus morhuaL. reared in commercial conditions

Strong phenotypic and genetic correlation between size and first maturity in Atlantic codGadus morhuaL. reared in commercial conditions

Landscape Genetics Reveals Population Subdivision in Bering Sea and Aleutian Islands Pacific Cod

Neutral and Nonneutral Genetic Markers Revealed the Presence of Inshore and Offshore Stock Components of Atlantic Cod in Greenland Waters

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