Characterization of 20 microsatellite marker loci in the red‐bellied brown lemur (<i>Eulemur rubriventer</i>)

Andriantompohavana, Rambinintsoa; Morelli, T. L.; Behncke, Sandra M.; Engberg, Shannon E.; Brenneman, Rick A.; Louis, Edward E.

doi:10.1111/j.1471-8286.2007.01815.x

Cited by 5 publications

(6 citation statements)

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“…We amplified 41 microsatellite markers: 18 loci were discovered in the E. cinereiceps genome (Tokiniaina et al ., ), 13 in the E. rubriventer genome (Andriantompohavana et al ., ) and 10 in the E. rufifrons genome. Five of the E. rufifrons markers were discovered by Jekielek & Strobeck (); the remaining markers are characterized here (Table S2).…”

Section: Methodsmentioning

confidence: 98%

Clinal variation in a brown lemur (Eulemurspp.) hybrid zone: Combining morphological, genetic and climatic data to examine stability

Delmore

Brenneman²,

Lei³

et al. 2013

J of Evolutionary Biology

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Studies of hybrid zones can inform our understanding of reproductive isolation and speciation. Two species of brown lemur (Eulemur rufifrons and E. cinereiceps) form an apparently stable hybrid zone in the Andringitra region of south-eastern Madagascar. The aim of this study was to identify factors that contribute to this stability. We sampled animals at 11 sites along a 90-km transect through the hybrid zone and examined variation in 26 microsatellites, the D-loop region of mitochondrial DNA, six pelage and nine morphological traits; we also included samples collected in more distant allopatric sites. Clines in these traits were noncoincident, and there was no increase in either inbreeding coefficients or linkage disequilibrium at the centre of the zone. These results could suggest that the hybrid zone is maintained by weak selection against hybrids, conforming to either the tension zone or geographical selection-gradient model. However, a closer examination of clines in pelage and microsatellites indicates that these clines are not sigmoid or stepped in shape but instead plateau at their centre. Sites within the hybrid zone also occur in a distinct habitat, characterized by greater seasonality in precipitation and lower seasonality in temperature. Together, these findings suggest that the hybrid zone may follow the bounded superiority model, with exogenous selection favouring hybrids within the transitional zone. These findings are noteworthy, as examples supporting the bounded superiority model are rare and may indicate a process of ecologically driven speciation without geographical isolation.

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Section: Methodsmentioning

confidence: 98%

Clinal variation in a brown lemur (Eulemurspp.) hybrid zone: Combining morphological, genetic and climatic data to examine stability

Delmore

Brenneman²,

Lei³

et al. 2013

J of Evolutionary Biology

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Section: Methodsmentioning

confidence: 99%

“…Samples were amplified at seven variable, unlinked microsatellite loci using previously published primers ( S2 Table ; [ 60 ]). Loci were selected for short fragment lengths, simple repeat motifs, and high allelic variability based on [ 60 ]. PCR conditions were carried out in 12.5 μ l total volume containing the Qiagen HotStarTaq Master Mix, 3.2 μ M bovine serum albumin, 0.8 μ M each of forward (fluorescently labeled) and reverse primers, and 2 μ l of total template DNA.…”

Section: Methodsmentioning

confidence: 99%

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Considering the Influence of Nonadaptive Evolution on Primate Color Vision

Jacobs

Bradley

2016

PLoS ONE

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Color vision in primates is variable across species, and it represents a rare trait in which the genetic mechanisms underlying phenotypic variation are fairly well-understood. Research on primate color vision has largely focused on adaptive explanations for observed variation, but it remains unclear why some species have trichromatic or polymorphic color vision while others are red-green color blind. Lemurs, in particular, are highly variable. While some species are polymorphic, many closely-related species are strictly dichromatic. We provide the first characterization of color vision in a wild population of red-bellied lemurs (Eulemur rubriventer, Ranomafana National Park, Madagascar) with a sample size (87 individuals; NX chromosomes = 134) large enough to detect even rare variants (0.95 probability of detection at ≥ 3% frequency). By sequencing exon 5 of the X-linked opsin gene we identified opsin spectral sensitivity based on known diagnostic sites and found this population to be dichromatic and monomorphic for a long wavelength allele. Apparent fixation of this long allele is in contrast to previously published accounts of Eulemur species, which exhibit either polymorphic color vision or only the medium wavelength opsin. This unexpected result may represent loss of color vision variation, which could occur through selective processes and/or genetic drift (e.g., genetic bottleneck). To indirectly assess the latter scenario, we genotyped 55 adult red-bellied lemurs at seven variable microsatellite loci and used heterozygosity excess and M-ratio tests to assess if this population may have experienced a recent genetic bottleneck. Results of heterozygosity excess but not M-ratio tests suggest a bottleneck might have occurred in this red-bellied lemur population. Therefore, while selection may also play a role, the unique color vision observed in this population might have been influenced by a recent genetic bottleneck. These results emphasize the need to consider adaptive and nonadaptive mechanisms of color vision evolution in primates.

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Ecological and genetic variables co‐vary with social group identity to shape the gut microbiome of a pair‐living primate

Whitney,

Mallott,

Diakiw

et al. 2024

American J Primatol

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Primates exhibit diverse social systems that are intricately linked to their biology, behavior, and evolution, all of which influence the acquisition and maintenance of their gut microbiomes (GMs). However, most studies of wild primate populations focus on taxa with relatively large group sizes, and few consider pair‐living species. To address this gap, we investigate how a primate's social system interacts with key environmental, social, and genetic variables to shape the GM in pair‐living, red‐bellied lemurs (Eulemur rubriventer). Previous research on this species suggests that social interactions within groups influence interindividual microbiome similarity; however, the impacts of other nonsocial variables and their relative contributions to gut microbial variation remain unclear. We sequenced the 16S ribosomal RNA hypervariable V4–V5 region to characterize the GM from 26 genotyped individuals across 11 social groups residing in Ranomafana National Park, Madagascar. We estimated the degree to which sex, social group identity, genetic relatedness, dietary diversity, and home range proximity were associated with variation in the gut microbial communities residing in red‐bellied lemurs. All variables except sex played a significant role in predicting GM composition. Our model had high levels of variance inflation, inhibiting our ability to determine which variables were most predictive of gut microbial composition. This inflation is likely due to red‐bellied lemurs' pair‐living, pair‐bonded social system that leads to covariation among environmental, social, and genetic variables. Our findings highlight some of the factors that predict GM composition in a tightly bonded, pair‐living species and identify variables that require further study. We propose that future primate microbiome studies should simultaneously consider environmental, social, and genetic factors to improve our understanding of the relationships among sociality, the microbiome, and primate ecology and evolution.

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Characterization of 20 microsatellite marker loci in the red‐bellied brown lemur (Eulemur rubriventer)

Cited by 5 publications

References 8 publications

Clinal variation in a brown lemur (Eulemurspp.) hybrid zone: Combining morphological, genetic and climatic data to examine stability

Clinal variation in a brown lemur (Eulemurspp.) hybrid zone: Combining morphological, genetic and climatic data to examine stability

Considering the Influence of Nonadaptive Evolution on Primate Color Vision

Ecological and genetic variables co‐vary with social group identity to shape the gut microbiome of a pair‐living primate

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