Characterization of 21 microsatellite marker loci in the ring-tailed lemur (Lemur catta)

Zaonarivelo, John R.; Andriantompohavana, Rambinintsoa; Shore, Gary E.; Engberg, Shannon E.; McGuire, Susie M.; Louis, Edward E.; Brenneman, Rick A.

doi:10.1007/s10592-006-9259-x

Cited by 7 publications

(6 citation statements)

References 9 publications

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“…All loci showed high levels of heterozygosity in both populations. Observed heterozygosity for each marker was comparable with—and in many cases greater than—that reported for these markers in other lemur studies, including those on Eulemur fulvus (Jekielek and Strobeck,1999) and captive and wild L. catta (Pastorini et al,2005; Zaonarivelo et al,2007). Additionally, all microsatellites were highly polymorphic, with allele number across loci ranging between 5 and 14 (Table 1).…”

Section: Resultssupporting

confidence: 81%

Evaluating ring‐tailed lemurs (Lemur catta) from southwestern Madagascar for a genetic population bottleneck

Parga

Sauther

Cuozzo

et al. 2011

American J Phys Anthropol

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In light of historical and recent anthropogenic influences on Malagasy primate populations, in this study ring-tailed lemur (Lemur catta) samples from two sites in southwestern Madagascar, Beza Mahafaly Special Reserve (BMSR) and Tsimanampetsotsa National Park (TNP), were evaluated for the genetic signature of a population bottleneck. A total of 45 individuals (20 from BMSR and 25 from TNP) were genotyped at seven microsatellite loci. Three methods were used to evaluate these populations for evidence of a historical bottleneck: M-ratio, mode-shift, and heterozygosity excess tests. Three mutation models were used for heterozygosity excess tests: the stepwise mutation model (SMM), two-phase model (TPM), and infinite allele model (IAM). M-ratio estimations indicated a potential bottleneck in both populations under some conditions. Although mode-shift tests did not strongly indicate a population bottleneck in the recent historical past when samples from all individuals were included, a female-only analysis indicated a potential bottleneck in TNP. Heterozygosity excess was indicated under two of the three mutation models (IAM and TPM), with TNP showing stronger evidence of heterozygosity excess than BMSR. Taken together, these results suggest that a bottleneck may have occurred among L. catta in southwestern Madagascar in the recent past. Given knowledge of how current major stochastic climatic events and human-induced change can negatively impact extant lemur populations, it is reasonable that comparable events in the historical past could have caused a population bottleneck. This evaluation additionally functions to highlight the continuing environmental and anthropogenic challenges faced by lemurs in southwestern Madagascar.

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Section: Resultssupporting

confidence: 81%

Evaluating ring‐tailed lemurs (Lemur catta) from southwestern Madagascar for a genetic population bottleneck

Parga

Sauther

Cuozzo

et al. 2011

American J Phys Anthropol

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“…Samples were amplified at up to 6 highly polymorphic loci, Lc5, Lc7 [Pastorini et al, 2005], 69HDZ267 [Zaonarivelo et al, 2007], Efr09 [Jekielek and Strobeck, 1999], L-2 [Merenlender, 1993] and Em12 [Pastorini, in preparation, as cited in Parga et al, 2012] via PCR. The 5 ′ ends of forward primers were fluorescently labeled and DNA was amplified using Qiagen Multiplex kits following Burrell [2009] using annealing temperatures specified in the literature.…”

mentioning

confidence: 99%

Genetic Diversity of the Ring-Tailed Lemur (Lemur catta) in South-Central Madagascar

Clarke

Gray²,

Gould³

et al. 2015

IJFP

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Madagascar's lemurs, now deemed the most endangered group of mammals, represent the highest primate conservation priority in the world. Due to anthropogenic disturbances, an estimated 10% of Malagasy forest cover remains. The endangered Lemur catta is endemic to the southern regions of Madagascar and now occupies primarily fragmented forest habitats. We examined the influence of habitat fragmentation and isolation on the genetic diversity of L. catta across 3 different forest fragments in south-central Madagascar. Our analysis revealed moderate levels of genetic diversity. Genetic differentiation among the sites ranged from 0.05 to 0.11. These data suggest that the L. catta populations within south-central Madagascar have not yet lost significant genetic variation. However, due to ongoing anthropogenic threats faced by ring-tailed lemurs, continued conservation and research initiatives are imperative for long-term viability of the species.

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“…Eleven microsatellite primers with high variability (Table S2) were selected among those developed by Pastorini, Fernando, Forstner, and Melnick () and Zaonarivelo et al (). PCR conditions for the 11 microsatellite primers were as follows: 10 ng DNA, 200 mM each dNTP, 12.5 pmol of each primer (one of each primer pair was end‐labeled with a fluorescent tag), 1.5 mM MgCl 2 , and 0.5 units of Taq DNA polymerase (hot master Taq 5 Prime).…”

Section: Methodsmentioning

confidence: 99%

Captive ring‐tailed lemur breeding in semi‐free ranging conditions and genetic parentage analysis

Breton

Thibault

Werts³

et al. 2019

Zoo Biology

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The ring‐tailed lemur (Lemur catta), one of the most iconic and widely recognized primates in the world, is threatened in its native range and classified as endangered by the International Union for Conservation of Nature. The global conservation strategy for the species includes ex situ conservation efforts led by zoological institutions in the framework of regional captive breeding programs. To maximize the conservation of genetic diversity and optimize breeding programs, an accurate pedigree must be established in captive populations. Our study documents the formation and subsequent monitoring of a large ring‐tailed lemur group in a French zoological institution. Based on staff's behavioral observations and DNA analyses using 11 microsatellite markers, we were able to document the survival and reproduction rates of the lemurs in this situation and reconstruct the pedigree of infants born in the institution. In total, 22 of the 28 imported animals as well as 4 locally born individuals gave birth to 58 infants of which 28 survived. The consistent genotypes obtained from the 53 sampled animals allowed us to identify 25 of the sires and 27 of the dams for the 28 surviving infants. A total of 12 different females and 14 different males produced the 28 surviving infants, suggesting that no individual dominates reproduction. This pioneering study has practical applications for the captive management of the species, which can be raised to the individual level instead of the group level.

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Characterization of 21 microsatellite marker loci in the ring-tailed lemur (Lemur catta)

Cited by 7 publications

References 9 publications

Evaluating ring‐tailed lemurs (Lemur catta) from southwestern Madagascar for a genetic population bottleneck

Evaluating ring‐tailed lemurs (Lemur catta) from southwestern Madagascar for a genetic population bottleneck

Genetic Diversity of the Ring-Tailed Lemur (Lemur catta) in South-Central Madagascar

Captive ring‐tailed lemur breeding in semi‐free ranging conditions and genetic parentage analysis

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