Characterization of 37 Breed-Specific Single-Nucleotide Polymorphisms in Sheep

Pariset, Lorraine; Cappuccio, I.; Ajmone‐Marsan, Paolo; Bruford, Michael William; Dunner, S.; Cortés, O.; Erhardt, G.; Prinzenberg, E.-M.; Katja, Gutscher; Joost, Stéphane; Pinto-Juma, Gabriela A.; Nijman, Isaäc J; Lenstra, Johannes A.; Pérez, Trinidad; Valentini, Alessio

doi:10.1093/jhered/esl020

Cited by 29 publications

(16 citation statements)

References 21 publications

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“…This finding is in agreement with results obtained for other European sheep breeds (Geriotti et al 2004). The second pattern, variant T, as described by Geriotti et al (2004) and Pariset et al (2006), occurred either with a decreased frequency of 10% to 36% in the Italian sheep population and elsewhere in the European population or, as in the present study, was not found.…”

Section: Resultssupporting

confidence: 61%

Technical note: A novel method for routine genotyping of the G allele of β-casein (CSN2) and T allele of κ-casein (CSN3) in a sheep population using LightCycler1

Sztankóová

Kyselová

Rychtářová

et al. 2011

Journal of Animal Science

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The goal of this work was to develop a protocol for rapid genotyping of A and G variants at the CSN2 locus and genotyping of T and C variants at the CSN3 locus in sheep breeds (Sumava and Valachian) by means of PCR and LightCycler analysis. The LightCycler technique combines rapid and efficient in vitro amplification of DNA in glass capillaries with melting curve analysis based on fluorescence resonance energy transfer for the sensitive detection of point mutation. The A variant had a greater frequency (Sumava, 0.778; Valachian, 0.835) than did variant G (Sumava, 0.222; Valachian, 0.165) in both sheep breeds. The CSN3 locus was found to be monomorphic, with no polymorphism identified in either population.

show abstract

Section: Resultssupporting

confidence: 61%

Technical note: A novel method for routine genotyping of the G allele of β-casein (CSN2) and T allele of κ-casein (CSN3) in a sheep population using LightCycler1

Sztankóová

Kyselová

Rychtářová

et al. 2011

Journal of Animal Science

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show abstract

“…Large scale European studies by the Econogene Consortium and others [13]–[16] involved some UK breeds, including the Exmoor Horn and Welsh Mountain shown subsequently to be geographically concentrated [8]. Isolated Scottish Island populations have also been studied in global surveys, including the numerically scarce breed of the Soay, and those in the Hebrides, Faeroe and Orkney [15], [17]–[19].…”

Section: Introductionmentioning

confidence: 99%

Genetic Distinctiveness of the Herdwick Sheep Breed and Two Other Locally Adapted Hill Breeds of the UK

2014

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There is considerable interest in locally adapted breeds of livestock as reservoirs of genetic diversity that may provide important fitness traits for future use in agriculture. In marginal areas, these animals contribute to food security and extract value from land unsuitable for other systems of farming. In England, close to 50% of the national sheep flock is farmed on grassland designated as disadvantaged areas for agricultural production. Many of these areas are in the uplands, where some native breeds of sheep continue to be commercially farmed only in highly localised geographical regions to which they are adapted. This study focuses on three of these breeds, selected for their adaptation to near identical environments and their geographical concentration in regions close to one another. Our objective has been to use retrotyping, microsatellites and single nucleotide polymorphisms to explore the origins of the breeds and whether, despite their similar adaptations and proximity, they are genetically distinctive. We find the three breeds each have a surprisingly different pattern of retrovirus insertions into their genomes compared with one another and with other UK breeds. Uniquely, there is a high incidence of the R0 retrotype in the Herdwick population, characteristic of a primitive genome found previously in very few breeds worldwide and none in the UK mainland. The Herdwick and Rough Fells carry two rare retroviral insertion events, common only in Texels, suggesting sheep populations in the northern uplands have a historical association with the original pin-tail sheep of Texel Island. Microsatellite data and analyses of SNPs associated with RXFP2 (horn traits) and PRLR (reproductive performance traits) also distinguished the three breeds. Significantly, an SNP linked to TMEM154, a locus controlling susceptibility to infection by Maedi-Visna, indicated that all three native hill breeds have a lower than average risk of infection to the lentivirus.

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“…In another study, a nucleotide change C/T was detected in Baluchi sheep breed at nt 271 of IGF -1 gene. The same mutation (in IGF -1 gene) has been detected in 8 different sheep breeds selected throughout Europe (Pariset et al, 2006) and in three Italian dairy sheep (Scata et al, 2010). These researchers found that allele T exerted a positive effect on maintaining a constant milk yield level during lactation.…”

Section: Discussionmentioning

confidence: 64%

Polymorphism of growth hormone gene and its association with wool traits in Egyptian sheep breeds

Farag¹,

Darwish²,

Darwish³

et al. 2016

Afr. J. Biotechnol.

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Growth hormone (GH) gene has been described as a candidate gene for marker-assisted selection in different farm animals. The present study was designed to identify the polymorphism in GH gene and its association with variation of wool traits in Egyptian sheep breeds. Wool and blood samples were collected from 42 animals including two breeds (Barki and Rahmani) and one crossbred (Rahmani x Awase). Measurements of wool traits were analyzed and involved staple strength (Str), staple length (STL), fiber diameter (FD) and clean fleece yield (CFW). DNA was extracted from blood samples and a 365-bp fragment from exon V was amplified by polymerase chain reaction (PCR). Single strand conformation polymorphisim (SSCP) analysis showed two conformational patterns. The pattern I was recorded to be more frequent (83.3, 92.86 and 90%) than pattern II (16.7, 7.14 and 10%) in Barki, Rahmani and crossbred, respectively. The sequence analysis showed one single nucleotide polymorphism (C/T). The pattern I (allele T) has been found to affect CFW and FD than pattern II (allele C). Whereas, C allele was more pronounced for Str and STL. These traits are the most important parameters determining commercial values of wool that are preferred for clothing or carpets industry. The nucleotide sequences of C and T alleles were submitted to GenBank and have the accession numbers: KT250511 and KT250512, respectively. In conclusion, the present results provide evidence that there is a single nucleotide polymorphism within GH gene in Egyptian sheep breeds. This mutation was found to have some effects on wool traits. Therefore our data show interesting prospects in future selection programs for improving wool industry.

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Characterization of 37 Breed-Specific Single-Nucleotide Polymorphisms in Sheep

Cited by 29 publications

References 21 publications

Technical note: A novel method for routine genotyping of the G allele of β-casein (CSN2) and T allele of κ-casein (CSN3) in a sheep population using LightCycler1

Technical note: A novel method for routine genotyping of the G allele of β-casein (CSN2) and T allele of κ-casein (CSN3) in a sheep population using LightCycler1

Genetic Distinctiveness of the Herdwick Sheep Breed and Two Other Locally Adapted Hill Breeds of the UK

Polymorphism of growth hormone gene and its association with wool traits in Egyptian sheep breeds

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