Characterization of 5S rRNA genes from mouse

Hallenberg, Charlotte; Nielsen, Joachim; Frederiksen, Sune

doi:10.1016/0378-1119(94)90277-1

Cited by 42 publications

(41 citation statements)

References 22 publications

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“…1B). Because the promoter elements of these genes are identical with the Pol III promoter consensus sequences, [37][38][39] all three genes are expected to be transcriptionally competent. The genes were placed in the same orientation at RL5.…”

Section: Resultsmentioning

confidence: 99%

“…RN5S copy number in clustered tandem arrays is highly variable; hundreds of consensus and 5S-related sequences are interspersed in the human and mouse genome. 16,38 A, B and C boxes are essential promoter elements. (C) Mean eGFp fluorescence of cassettes tested for anti-silencing/barrier activity four weeks post-transfection: p212, no silencer element; pYB, with silencer element; a mouse RN5S (5S) gene; a human Alu element; a single mouse tRNA (Glu) gene.…”

Section: Introductionmentioning

confidence: 99%

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tRNA genes protect a reporter gene from epigenetic silencing in mouse cells

Ebersole

Kim²,

Samoshkin³

et al. 2011

Cell Cycle

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

tRNA genes protect a reporter gene from epigenetic silencing in mouse cells

Ebersole

Kim²,

Samoshkin³

et al. 2011

Cell Cycle

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“…Thus, the NTSs seem to be subject to rapid evolution, which makes this region both an important resource for studies concerning the organization and evolution of multigene families and genomes and also a marker to trace recent events of evolution. The intense dynamism of 5S rDNA repeats generates variant classes of 5S rDNA, which have been reported in the genomes of several vertebrates, from lampreys to mammals (Komiya et al, 1986 ;Hallenberg et al, 1994;Frederiksen et al, 1997). Particularly in marine and freshwater ray-finned fishes, vast numbers of structural and functional data have demonstrated the occurrence of a dual size-class pattern of organization of the 5S rDNA (Moran et al, 1996 ;Ce´spedes et al, 1999 ;Rocco et al, 1999;Deiana et al, 2000 ;Martins & Galletti, 2001 a ;Wasko et al, 2001 ;Martins et al, 2002 ;Messias et al, 2003 ;Tigano et al, 2004 ;Alves-Costa et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Molecular organization of 5S rDNA in sharks of the genusRhizoprionodon: insights into the evolutionary dynamics of 5S rDNA in vertebrate genomes

Pinhal¹,

Araki²,

Gadig³

et al. 2009

Genet. Res.

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SummaryIn this study, we attempted a molecular characterization of the 5S rDNA in two closely related species of carcharhiniform sharks, Rhizoprionodon lalandii and Rhizoprionodon porosus, as well as a further comparative analysis of available data on lampreys, several fish groups and other vertebrates. Our data show that Rhizoprionodon sharks carry two 5S rDNA classes in their genomes : a short repeat class (termed class I) composed of y185 bp repeats, and a large repeat class (termed class II) arrayed in y465 bp units. These classes were differentiated by several base substitutions in the 5S coding region and by completely distinct non-transcribed spacers (NTS). In class II, both species showed a similar composition for both the gene coding region and the NTS region. In contrast, class I varied extensively both within and between the two shark species. A comparative analysis of 5S rRNA gene sequences of elasmobranchs and other vertebrates showed that class I is closely related to the bony fishes, whereas the class II gene formed a separate cartilaginous clade. The presence of two variant classes of 5S rDNA in sharks likely maintains the tendency for dual ribosomal classes observed in other fish species. The present data regarding the 5S rDNA organization provide insights into the dynamics and evolution of this multigene family in the fish genome, and they may also be useful in clarifying aspects of vertebrate genome evolution.

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“…The promoter for 5S rDNA is of type 1 and consists of BoxA, an Internal Element (IE), and BoxC; that is found within the transcribed region. 7 These three elements are grouped in the Internal Control Region (ICR) 8 and in elasmobranchs (shark, rays, and skates) have been reported to be respectively at positions 50 -64, 67 -72, and 80 -97 bp of the transcript. 9,10 The transcribed region also contains a termination signal consisting of a poly-T relic, that is still recognized by RNA polymerases III during transcription.…”

Section: Introductionmentioning

confidence: 99%

Molecular organization of the 5S rDNA gene type II in elasmobranchs

et al. 2015

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The 5S rDNA gene is a non-coding RNA that can be found in 2 copies (type I and type II) in bony and cartilaginous fish. Previous studies have pointed out that type II gene is a paralog derived from type I. We analyzed the molecular organization of 5S rDNA type II in elasmobranchs. Although the structure of the 5S rDNA is supposed to be highly conserved, our results show that the secondary structure in this group possesses some variability and is different than the consensus secondary structure. One of these differences in Selachii is an internal loop at nucleotides 7 and 112. These mutations observed in the transcribed region suggest an independent origin of the gene among Batoids and Selachii. All promoters were highly conserved with the exception of BoxA, possibly due to its affinity to polymerase III. This latter enzyme recognizes a dT 4 sequence as stop signal, however in Rajiformes this signal was doubled in length to dT 8. This could be an adaptation toward a higher efficiency in the termination process. Our results suggest that there is no TATA box in elasmobranchs in the NTS region. We also provide some evidence suggesting that the complexity of the microsatellites present in the NTS region play an important role in the 5S rRNA gene since it is significantly correlated with the length of the NTS.

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Characterization of 5S rRNA genes from mouse

Cited by 42 publications

References 22 publications

tRNA genes protect a reporter gene from epigenetic silencing in mouse cells

tRNA genes protect a reporter gene from epigenetic silencing in mouse cells

Molecular organization of 5S rDNA in sharks of the genusRhizoprionodon: insights into the evolutionary dynamics of 5S rDNA in vertebrate genomes

Molecular organization of the 5S rDNA gene type II in elasmobranchs

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