Characterization of 7-ethoxycoumarin-O-deethylase from malathion resistant and susceptible strains of Drosophila melanogaster

Patil, T.N.; Morton, R. A.; Singh, Rama S.

doi:10.1016/0020-1790(90)90024-o

Cited by 9 publications

(3 citation statements)

References 22 publications

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“…Limiting mineral and vitamin intake as well as protein consumption for this species also failed to affect cytochrome P450 monooxygenase activity (Lindroth et al 1991), although esterase and carbonyl reductase activities were reduced. In another study in which nutrient effects on detoxification were considered, Patil et al (1990) reported that the nutritional state of Drosophila melanogasteraffects its P450 activity; feeding on a nutritionally incomplete agar-1% sucrose diet reduced 7 -ethoxycoumarin-0-deethylase activity 20-40% relative to larvae fed nutritionally complete diet.…”

Section: Introductionmentioning

confidence: 99%

Costs of Inducible Defense: Protein Limitation, Growth, and Detoxification in Parsnip Webworms

Berenbaum

Zangerl

1994

Ecology

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Although xanthotoxin, a linear furanocoumarin, is toxic to a wide variety of lepidopterans, the parsnip webworm, Depressaria pastinacella (Lepidoptera: Oecophoridae), is restricted to feeding on only two genera of Apiaceae, both of which contain large quantities of this toxin. The cost of cytochrome P450—mediated detoxification of xanthotoxin by this oligophagous species was examined in two artificial diet experiments. In the first experiment, growth rates, silkspinning rates, and detoxification capacity of larvae were measured after they had consumed diets ranging in protein content from 0 to 100% of the amount required for maximum growth. As protein became progressively more limited, growth rates declined progressively while silkspinning rates and detoxification capacity remained unchanged; only when dietary protein was completely absent did silkspinning rates and xanthotoxin metabolism rates decline. These findings suggest that xanthotoxin detoxification capacity is maintained at the expense of growth. In the second experiment, growth rates, silkspinning rates, and xanthotoxin metabolism were measured in larvae that consumed diets with or without xanthotoxin in the presence or absence of protein. As in the previous experiment, the absence of protein led to significant reductions in growth, silkspinning, and detoxification. The presence of xanthotoxin in the diet resulted in an increase in the rates of xanthotoxin metabolism and silkspinning but did not have any effect on growth rates. Inasmuch as there were no interactions between protein and xanthotoxin treatments, induction of xanthotoxin metabolism by xanthotoxin was not affected by protein limitation. The fact that xanthotoxin metabolism is induced almost threefold in the absence of dietary protein, conditions that result in reduction of growth rates by almost 80%, is also consistent with the greater importance of detoxification capacity relative to growth over the short term. Due to its highly oligophagous habits, the parsnip webworm is unlikely to encounter host plants tissue entirely lacking furanocoumarins; circumstances under which the inability to respond to the presence of xanthotoxin in the diet by increasing rates of its metabolism may never exist. Thus, maintaining high and inducible levels of cytochrome P450—mediated xanthotoxin metabolism may be regarded as one of the costs associated with the highly specialized diet of D. pastinacella.

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Section: Introductionmentioning

confidence: 99%

Costs of Inducible Defense: Protein Limitation, Growth, and Detoxification in Parsnip Webworms

Berenbaum

Zangerl

1994

Ecology

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“…al. (12) was used for the measurement of 7-ethoxycoumarin (ECOD) activity. Both reactions were performed at +25"C.…”

Section: Enzymes Extracts Preparation and Assaymentioning

confidence: 99%

The effect of the cytochrome P‐450 system inducers on the development of drosophila melanogaster

Fuchs¹,

Spiegelman²,

Belitsky³

1993

J. Biochem. Toxicol.

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D. melanogaster development was markedly retarded and its survival decreased by larvae treatment with compounds being strong inducers of the cytochrome P-450 2B in mammals--phenobarbital (PB*), perfluorodecaline (PFD), transtilbene oxide (TSO), and triphenyldioxane (TPD). At the same time, the weak inducer hexobarbital or the selective cytochrome P-450 inducer in mice but not in rats 1,4-bis[2-(dichloropyridyl-oxy)]-benzene (DPB) did not affect the larvae development. The cytochrome P-450 1A1 inducers benzo(a)anthracene (BA) and beta-naphtoflavone (BNF) were also not effective. The toxicity of phenobarbital was shown to be decreased by the cytochrome P-450 inhibitor piperonyl butoxide by adding 20-hydroxyecdysone or by treatment with aminophylline--the indirect enhancer of ecdysone production in the larval prothoracic gland. The hypothesis of the moulting hormone degradation as the cause of elevated larvae mortality resulting from the induced high mixed function oxidase activity has been discussed.

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“…Polyphagy, or the ability to utilize hosts from many different plant families, is a trait commonly found among herbivorous invasive species and insect pests (Cho et al, 2008;Jeschke and Strayer, 2008;Nahrung and Swain, 2015;Pearce et al, 2017). Adaptations associated with polyphagy include the ability to tolerate a diversity of plant secondary compounds in addition to nutritional variability; however, these two traits are likely intimately connected, given that detoxification is fundamentally limited by nutritional constraints (Lindroth et al, 1990(Lindroth et al, , 1991Patil et al, 1990;Berenbaum and Zangerl, 1994;Jensen et al, 2016). Nutritional plasticity allows invasive and pest species to utilize wider host ranges and invade new habitats.…”

Section: Introductionmentioning

confidence: 99%

Gene-by-environment interactions in agricultural pest management: population effects on diet-Btinteractions in a caterpillar

Deans

Sword

Behmer

et al. 2020

Preprint

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Given that plant nutrient content is both spatially and temporally dynamic (Lenhart et al., 2015; Deans et al., 2016, 2018), insect herbivores are exposed to an incredible amount of nutritional variability. This variability can constrain insects to feeding on sub-optimal resources, but it can also provide an opportunity for insects to regulate their intake of specific nutrients to obtain an optimal balance. Nutrient regulation has implications for pest control strategies in agricultural systems, as the nutritional state of pest species may impact their susceptibility to insecticides. Deans et al. (2017) showed that diet macronutrient balance has significant effects on the susceptibility of Helicoverpa zea larvae to Cry1Ac, an endotoxin expressed in transgenic Bt crops. This was demonstrated using a highly inbred laboratory strain of H. zea, limiting the applicability of these results to field populations that encompass greater genetic diversity. In this study, we assessed the impact of field-relevant macronutrient variability on the efficacy of two Bt endotoxins, Cry1Ab and Cry1Ac, using three field populations collected from different geographic regions. This was done to further understand the impact of nutritional variability on Bt susceptibility and also to determine the relevance of these effects in the field. While we saw limited differences in Cry susceptibility across populations, dietary effects were highly variable. Across populations there were distinct population-level differences in the interactions between Cry concentration and diet, the type of Cry toxin impacted by diet, and the treatment diet that produced optimal survival and performance. These results show that nutrition can have strong impacts on Bt susceptibility but also that these impacts are strongly affected by genetic background in H. zea. To accurately assess Bt susceptibility in the field, including resistance monitoring, bioassay methods should incorporate the appropriate nutritional parameters and be as localized as possible.

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Characterization of 7-ethoxycoumarin-O-deethylase from malathion resistant and susceptible strains of Drosophila melanogaster

Cited by 9 publications

References 22 publications

Costs of Inducible Defense: Protein Limitation, Growth, and Detoxification in Parsnip Webworms

Costs of Inducible Defense: Protein Limitation, Growth, and Detoxification in Parsnip Webworms

The effect of the cytochrome P‐450 system inducers on the development of drosophila melanogaster

Gene-by-environment interactions in agricultural pest management: population effects on diet-Btinteractions in a caterpillar

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