Characterization of a 36 kDa antigenic protein of fish-specific monoclonal-antibody 8F5

Chen, Yi-Tien; Hsieh, Yun-Hwa Peggy

doi:10.1016/j.foodchem.2022.132149

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…Furthermore, Lefèvre et al described a case of an allergic reaction after consuming caviar and affirmed by immunoblotting that the patient was allergic to a 26 kDa protein in caviar . Tropomyosin, a common allergen in crustaceans, was detected as a fish allergen by a fish-specific monoclonal antibody …”

Section: Fish Allergensmentioning

confidence: 99%

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Fish Allergy: A Review of Clinical Characteristics, Mechanism, Allergens, Epitopes, and Cross-Reactivity

Sun,

Luo,

Chen

et al. 2024

ACS Food Sci. Technol.

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Fish is one of eight foods that can cause severe allergic reactions. The growing consumption of fish and fish derivatives contributes to an increase in the incidence of fish allergies. Distinct regional variations in fish allergies are evident with higher prevalence rates observed in coastal countries, which may be related to the high consumption of local fish in these regions. The diagnostic methods of fish allergies include in vivo and in vitro detection, and the skin prick test is one of the most common screening methods. To date, several fish allergens have been identified, including parvalbumin, enolase, aldolase, collagen, and other allergens. Despite the homologous structure of parvalbumin from different fish species, variations in the IgE epitope may be one of the reasons leading to the complexity of fish allergies. To further understand the mechanisms of fish allergies, this review summarizes the recent literature and focuses on the clinical characteristics, mechanism, allergens, epitopes, and cross-reactivity.

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Section: Fish Allergensmentioning

confidence: 99%

“…68 Tropomyosin, a common allergen in crustaceans, was detected as a fish allergen by a fish-specific monoclonal antibody. 69…”

Section: Enolase and Aldolasementioning

confidence: 99%

Fish Allergy: A Review of Clinical Characteristics, Mechanism, Allergens, Epitopes, and Cross-Reactivity

Sun,

Luo,

Chen

et al. 2024

ACS Food Sci. Technol.

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Composition, structural configuration, and antigenicity of Atlantic cod (Gadus morhua) tropomyosin

et al. 2023

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Quantitative Assessment of Bony Fish Tropomyosin Using a Monoclonal Antibody Competitive ELISA

Zhao,

Jiang,

Bai

et al. 2024

J. Agric. Food Chem.

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Bony fish is one of the big-nine allergenic foods in the US. This study established a monoclonal antibody (mAb)based indirect competitive enzyme-linked immunosorbent assay (ELISA) for the detection of bony fish tropomyosin (TM), a fish allergen. Immunoassay (Western blot and ELISA) was performed to characterize anti-TM mAb8F5 (target configuration, immunoaffinity, and species selectivity). Atlantic cod (Gadus morhua) tropomyosin (C TM ) and mAb8F5 were used for assay development. The immunosignal was captured by two light sources, i.e., colorimetry (cELISA) and luminescence (lELISA). During assay validation, cELISA had a wider working range (1−250 ng/μL) than lELISA (0.5−62.5 ng/μL). However, lELISA showed better sensitivity because of its lower half-maximal inhibitory concentration (IC 50 , 8.8 ng/μL) than cELISA (37.1 ng/μL). The optimal cELISA was applied to measure C TM -equivalents in 21 bony fish samples (0.03−4.2 mg/g) and laboratory-spiked samples (recovery rates of C TM -spiked shrimp samples ranged from 104.7 to 120.8%). lELISA was used to quantify 5.25 μg C TM residues on four food contact surfaces (3.4−6.9 μg from C TM residues and 3.2−4.8 μg from Atlantic cod were detected). This immunoassay has the potential to surveil undeclared allergenic bony fish residues and validate the hygiene of food contact surfaces.

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Characterization of a 36 kDa antigenic protein of fish-specific monoclonal-antibody 8F5

Cited by 3 publications

References 18 publications

Fish Allergy: A Review of Clinical Characteristics, Mechanism, Allergens, Epitopes, and Cross-Reactivity

Fish Allergy: A Review of Clinical Characteristics, Mechanism, Allergens, Epitopes, and Cross-Reactivity

Composition, structural configuration, and antigenicity of Atlantic cod (Gadus morhua) tropomyosin

Quantitative Assessment of Bony Fish Tropomyosin Using a Monoclonal Antibody Competitive ELISA

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