Characterization of a Cadmium-Binding Complex of Cabbage Leaves

Wagner, George J.

doi:10.1104/pp.76.3.797

Cited by 74 publications

(60 citation statements)

References 12 publications

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“…Complexation to low molecular weight organic compounds (<10 kD) was also shown to play a role in tolerance to Ni (Lee et al, 1977). Cadmium, a potentially toxic metal, has been shown to accumulate in plants where it is detoxified by binding to phytochelatins (Wagner 1984;Steffens, 1990;Cobbett and Goldsbrough, 1999), a family of thiol (SH)-rich peptides (Rauser,1990). Metallothioneins (MT), identified in numerous animals and more recently in plants and bacteria (Kägi, 1991), are also compounds (proteins) with heavy metalbinding properties (Tomsett et al, 1992).…”

Section: Plant Mechanisms For Metal Detoxificationmentioning

confidence: 99%

Phytoextraction of Metals from Contaminated Soil: A Review of Plant/Soil/Metal Interaction and Assessment of Pertinent Agronomic Issues

Lasat¹

1999

Journal of Hazardous Substance Research

241

201

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Phytoremediation is an emerging technology that employs the use of higher plants for the cleanup of contaminated environments. Fundamental and applied research have unequivocally demonstrated that selected plant species possess the genetic potential to remove, degrade, metabolize, or immobilize a wide range of contaminants. Despite this tremendous potential, phytoremediation is yet to become a commercial technology. Progress in the field is precluded by limited knowledge of basic plant remedial mechanisms. In addition, the effect of agronomic practices on these mechanisms is poorly understood. Another limitation lies within the very biological nature of this novel approach. For example, potential for phytoremediation depends upon the interaction among soil, contaminants, microbes, and plants. This complex interaction, affected by a variety of factors, such as climatic conditions, soil properties, and site hydro-geology, argues against generalization, and in favor of site-specific phytoremediating practices. Thus, an understanding of the basic plant mechanisms and the effect of agronomic practices on plant/soil/contaminant interaction would allow practitioners to optimize phytoremediation by customizing the process to site-specific conditions.Remediation of metal-contaminated soil faces a particular challenge. Unlike organic contaminants, metals cannot be degraded. Commonly, decontamination of metal-contaminated soils requires the removal of toxic metals. Recently, phytoextraction, the use of plants to extract toxic metals from contaminated soils, has emerged as a cost-effective, environment-friendly cleanup alternative. In this paper, we review the processes and mechanisms that allow plants to remove metals from contaminated soils and discuss the effects of agronomic practices on these processes.

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Section: Plant Mechanisms For Metal Detoxificationmentioning

confidence: 99%

Phytoextraction of Metals from Contaminated Soil: A Review of Plant/Soil/Metal Interaction and Assessment of Pertinent Agronomic Issues

Lasat¹

1999

Journal of Hazardous Substance Research

241

201

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“…Although occurrences of metal tolerances in higher plant species (Bartolf, Brennan & Price, 1980; plants have been widely reported (Antonovics, Rauser & Curvetto, 1980;Casterline & Barnett, Bradshaw & Turner, 1971;Bradshaw & McNeilly, 1982;Wagner & Trotter, 1982;Wagner, 1984). 1981), the physiological mechanisms responsible for Such proteins haxe been suggested to play a key role them are poorly understood (Thurman, 1981; in the metal tolerance of certain edaphic ecotypes Thurman & Collins, 1983;Woolhouse, 1983).…”

Section: Introduction 974^ Qj^q) J^^^^ Y^^^^^ F^^^jj!^ ^^^ ^ Nummentioning

confidence: 99%

Evidence against a key role for metallothionein‐like protein in the copper tolerance mechanism of Deschampsia cespitosa (L.) Beauv.

Schultz

Hutchinson

1988

New Phytologist

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SUMMARYThe role of sulphur and copper-inducible thiol-rich protein in the copper tolerance of the grass Deschampsia cespitosa (L.) Beauv. was investigated. Sulphur deficiency, induced by growinfi; tillers in nutrient solution without sulphur had no eftect on the copper tolerance of two clones from a site contaminated with copper and nickel. In contrast sulphur deficiency increased the susceptibility of two non-tolerant clones from an uncontaminated site to copper toxicity. T'olerance of a copper-tolerant clone screened from seed of a non-tolerant population was unaffected by sulphur deficiency. Sulpl-iur deficiency reduced the amount of copper-inducible tliiol-ricli protein in roots of both the tolerant and non-tolerant clones exposed to excess copper. Roots of the tolerant clones fjrown in micronutrient copper solutions without sulphur for 26 d and then exposed to 1-61 //M copper for a further 4 d both produced 7S",, less copper-inducible thiol-rich protein than those grown with sulphur. In an experiment with one of the tolerant clones, the amount of thiol-rich protein did not increase between 4 and 30 d of exposure to excess copper despite a 43 ",> increase in copper in the cell-free extract. Sulphur deficiency reduced tbe amount of tbiol-rich protein to a lesser extent in the non-tolerant clones, with a 57",, decrease in one clone and a 31 "" decrease in the other. In the presence of sulphur both non-tolerant cloties produced coi-isiderably more thiol-rich protein when exposed to excess copper than did the tolerant clones.

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“…These mechanisms mainly imply modifications of membrane and cell wall properties, compartmentation ofmetals in vacuoles, immobilization of metals in cell walls, and adaptive mechanisms of metabolic and enzymic nature (12,30). In addition, inducible metallothionein or metallothionein-like complexes have also been postulated to be involved in the mechanism of tolerance of certain plants to copper (12,18) and cadmium (28).…”

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confidence: 99%

Increased Levels of Peroxisomal Active Oxygen-Related Enzymes in Copper-Tolerant Pea Plants

Palma¹,

Gómez²,

Yáñez³

et al. 1987

Plant Physiol.

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The effect in vivo of high nutrient levels of copper (240 micromolar) on the activity of different metalloenzymes conaining Cu, Mn, Fe, and Zn, distributed in chloroplasts, peroxisomes, and mitochondria, was studied in leaves of two varieties ofPisum satiRvum L. plants with different sensitivity to copper. The metalloenzymes studied were: cytochrome c oxidase, Mn-superoxide dismutase (Mn-SOD) and Cu,Zn-superoxide dismutase I (Cu,Zn-SOD I1), for mitochondria; catalase and Mn-SOD, for peroxisomes; and isozyme C,Zn-SOD II for chloroplasts. The activity of mitochondrial SOD isozymes (Mn-SOD and Cu,Zn-SOD I) was very similar in Cu-tolerant and Cu-sensitive plants, whereas cytochrome c oxidase was lower in Cu-sensitive plants. Chloroplastid Cu,Zn-SOD activity was the same in the two plant varieties. In contrast, the peroxisomal Mn-SOD activity was considerably higher in Cu-tolerant than in Cu-sensitive plants, and the activity of catalase was also increased in peroxisomes of Cu-tolerant plants. The However, there is a paucity of information on the activity response of metalloenzymes present in different cell compartments of plant leaves to an enhanced pool of nutrient copper. Comparative studies of the activity of metalloenzymes in different cell organelles of copper tolerant and nontolerant plants grown in high Cu nutrient levels would allow deeper insights into the molecular mechanisms ofintracellular responses to plant toxicity and metal tolerance. These types of studies may also give information on possible alterations of metalloenzyme characteristics in metal-tolerant plants and on the adaptive nature of enzymes to metal stress in certain plants.The induction of a Mn-SOD3 in leaves of pea plants by high nutrient levels of zinc and manganese has been recently demonstrated (7), as well as the induction of Fe-superoxide dismutases in lemon leaves by iron (25). This suggested a possible involvement of the metalloenzyme family of SODs in the mechanism of plant tolerance to metal toxicity. SODs (EC 1.15.1.1) are a group of metalloenzymes that catalyze the disproportionation of superoxide free radicals (02O), produced in certain cellular loci, and appear to play an important role in protecting cells against the indirect lethal effect of O2 radicals (17). There are essentially three types of SODs containing either Mn, Fe, or Cu plus Zn as metal prosthetic groups (17).Leaves of pea plants contain three electrophoretically distinct SODs, a Mn-containing and two Cu,Zn-containing isozymes, named I and II in order of increasing mobility (7). The isozyme Mn-SOD has been fully characterized (24), as well as the two pea leaf Cu,Zn-SODs (9). By studies of subcellular distribution of SODs in pea leaves the presence of Mn-SOD has been demonstrated both in mitochondria and peroxisomes (6, 19) whereas isozyme Cu,Zn-SOD II was located in chloroplasts (15), and Cu,Zn-SOD I appeared to be distributed between mitochondria and the cytosol (19). This location of SOD isozymes in different cell compartments makes this metalloenzym...

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Characterization of a Cadmium-Binding Complex of Cabbage Leaves

Cited by 74 publications

References 12 publications

Phytoextraction of Metals from Contaminated Soil: A Review of Plant/Soil/Metal Interaction and Assessment of Pertinent Agronomic Issues

Phytoextraction of Metals from Contaminated Soil: A Review of Plant/Soil/Metal Interaction and Assessment of Pertinent Agronomic Issues

Evidence against a key role for metallothionein‐like protein in the copper tolerance mechanism of Deschampsia cespitosa (L.) Beauv.

Increased Levels of Peroxisomal Active Oxygen-Related Enzymes in Copper-Tolerant Pea Plants

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